犬肾细胞系/IgR;MDCK/IgR
上皮细胞样
贴壁生长
该细胞为MDCK细胞的克隆系。
MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts) 10%FBS
1:3传代,2-3天传一代
C6
基础培养基+5%DMSO+20%FBS
公司细胞的简洁信息:
(1)储存:液氮。
(2)运输:干冰。
(3)用途:只可用于科研。
(4)第二代培养末期液氮冻存。
(5)犬肾细胞系/IgR;MDCK/IgR每冻存管细胞数:>5×105 cells/1ml。
(6)不含有HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌。
收到犬肾细胞系/IgR;MDCK/IgR后的处理:
1)收到细胞后,首先观察瓶身是否完好(注意有无缝隙,裂痕)。
2)显微镜下观察细胞的生长状况,有无细胞漂浮。
3)用新洁尔灭消毒瓶口及瓶身。
4)打开瓶口,再次用新洁尔灭消毒瓶口(可能会有液体溢出,注意不要碰到液体),酒精灯烤瓶口消毒。
5)将培养液转移至50ml离心管或广口瓶中(若细胞漂浮严重,离心培养基,1000g*5分钟,将离心后的培养基转移至另一个大离心管中,留一点吹打细胞沉淀成悬液,回收细胞至原培养瓶),若漂浮不严重,亦离心一下。
6)在原培养瓶中留一部分原装培养液,再补加自己新配的培养基至适当容积,例如4ml,让细胞适应一下环境。 当细胞长到70-80%,冻存大部分,小部分传代。
A16725 真核翻译起始因子4A2(EIF4A2)酶联免疫试剂盒 ,英文名: ELISA Kit for Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16726 DNA指导聚合酶γ1(POLγ1)酶联免疫试剂盒 ,英文名: ELISA Kit for Polymerase DNA Directed Gamma 1 (POLg1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16727 腺苷A2b受体(ADORA2b)酶联免疫试剂盒 ,英文名: ELISA Kit for Adenosine A2b Receptor (ADORA2b) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A16728 磷脂酰肌醇结合网格蛋白装配蛋白(PICALM)酶联免疫试剂盒 ,英文名: ELISA Kit for Phosphatidylinositol Binding Clathrin Assembly Protein (PICALM) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16729 氧化应激诱导生长抑制因子1(OSGIN1)酶联免疫试剂盒 ,英文名: ELISA Kit for Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16730 B-Raf原癌基因丝氨酸/苏氨酸蛋白激酶(BRAF)酶联免疫试剂盒 ,英文名: ELISA Kit for B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16731 内质网氨肽酶2(ERAP2)酶联免疫试剂盒 ,英文名: ELISA Kit for Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16732 甘氨酸脱氢酶(GLDC)酶联免疫试剂盒 ,英文名: ELISA Kit for Glycine Dehydrogenase (GLDC) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A16733 甲状腺转录因子1(TITF1)酶联免疫试剂盒 ,英文名: ELISA Kit for Thyroid Transcription Factor 1 (TITF1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16734 肿瘤蛋白p53结合蛋白1(TP53BP1)酶联免疫试剂盒 ,英文名: ELISA Kit for Tumor Protein p53 Binding Protein 1 (TP53BP1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16735 突触囊泡蛋白样蛋白2(SYTL2)酶联免疫试剂盒 ,英文名: ELISA Kit for Synaptotagmin Like Protein 2 (SYTL2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16736 NAP关联蛋白(SNAPAP)酶联免疫试剂盒 ,英文名: ELISA Kit for SNAP Associated Protein (SNAPAP) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16737 副肿瘤抗原MA2(PNMA2)酶联免疫试剂盒 ,英文名: ELISA Kit for Paraneoplastic Antigen MA2 (PNMA2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16738 异柠檬酸脱氢酶2(IDH2)酶联免疫试剂盒 ,英文名: ELISA Kit for Isocitrate Dehydrogenase 2, mitochondrial (IDH2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16739 亨廷顿关联蛋白1(HAP1)酶联免疫试剂盒 ,英文名: ELISA Kit for Huntingtin Associated Protein 1 (HAP1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16740 G蛋白α抑制活性多肽3(GNαI3)酶联免疫试剂盒 ,英文名: ELISA Kit for G Protein Alpha Inhibiting Activity Polypeptide 3 (GNaI3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16741 葡萄糖-6-磷酸酶催化亚基(G6PC)酶联免疫试剂盒 ,英文名: ELISA Kit for Glucose-6-Phosphatase, Catalytic (G6PC) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16742 脆性X智力迟钝蛋白1(FMR1)酶联免疫试剂盒 ,英文名: ELISA Kit for Fragile X Mental Retardation 1 (FMR1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16743 丝聚蛋白(FLG)酶联免疫试剂盒 ,英文名: ELISA Kit for Filaggrin (FLG) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A16744 Zeste同源物增强子1(EZH1)酶联免疫试剂盒 ,英文名: ELISA Kit for Enhancer Of Zeste Homolog 1 (EZH1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16745 Zeste同源物增强子1(EZH1)酶联免疫试剂盒 ,英文名: ELISA Kit for Enhancer Of Zeste Homolog 1 (EZH1) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A16746 ER脂质Raft关联蛋白2(ERLIN2)酶联免疫试剂盒 ,英文名: ELISA Kit for ER Lipid Raft Associated Protein 2 (ERLIN2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16747 肿瘤关联糖蛋白72(TAG72)酶联免疫试剂盒 ,英文名: ELISA Kit for Tumor Associated Glycoprotein 72 (TAG72) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16748 含亮氨酸丰富重复蛋白3C(LRRC3C)酶联免疫试剂盒 ,英文名: ELISA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A16749 ER脂质Raft关联蛋白1(ERLIN1)酶联免疫试剂盒 ,英文名: ELISA Kit for ER Lipid Raft Associated Protein 1 (ERLIN1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16750 丝裂原激活蛋白激酶14(MAPK14)酶联免疫试剂盒 ,英文名: ELISA Kit for Mitogen Activated Protein Kinase 14 (MAPK14) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A16751 周期素依赖性激酶2(CDK2)酶联免疫试剂盒 ,英文名: ELISA Kit for Cyclin Dependent Kinase 2 (CDK2) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A16752 地塞米松诱导蛋白(DEXI)酶联免疫试剂盒 ,英文名: ELISA Kit for Dexamethasone Induced Protein (DEXI) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16753 脂肪细胞膜关联蛋白(APMAP)酶联免疫试剂盒 ,英文名: ELISA Kit for Adipocyte Plasma Membrane Associated Protein (APMAP) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16754 Bcl2相关髓细胞白血病序列1(MCL1)酶联免疫试剂盒 ,英文名: ELISA Kit for Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A16755 肾上腺素能受体β1(ADRβ1)酶联免疫试剂盒 ,英文名: ELISA Kit for Adrenergic Receptor Beta 1 (ADRb1) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A16756 磷酸二酯酶3A(PDE3A)酶联免疫试剂盒 ,英文名: ELISA Kit for Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A16757 CD14分子(CD14)酶联免疫试剂盒 ,英文名: ELISA Kit for Cluster Of Differentiation 14 (CD14) ,适用种属: Sus scrofa; Porcine (Pig) ,规格: 48T/96T
A16758 拓扑异构酶Ⅱ(TOP2)酶联免疫试剂盒 ,英文名: ELISA Kit for Topoisomerase II (TOP2) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A16759 叉头框蛋白O1(FOXO1)酶联免疫试剂盒 ,英文名: ELISA Kit for Forkhead Box Protein O1 (FOXO1) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A16760 拓扑异构酶Ⅱ(TOP2)酶联免疫试剂盒 ,英文名: ELISA Kit for Topoisomerase II (TOP2) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A16761 激活素A受体Ⅰ(ACVR1)酶联免疫试剂盒 ,英文名: ELISA Kit for Activin A Receptor Type I (ACVR1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16762 L1-细胞粘附分子(L1CAM)酶联免疫试剂盒 ,英文名: ELISA Kit for L1-Cell Adhesion Molecule (L1CAM) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A16763 细胞色素P450家族成员2E1(CYP2E1)酶联免疫试剂盒 ,英文名: ELISA Kit for Cytochrome P450 2E1 (CYP2E1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16764 胱天蛋白酶1(CASP1)酶联免疫试剂盒 ,英文名: ELISA Kit for Caspase 1 (CASP1) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A16765 胰岛素样生长因子1受体(IGF1R)酶联免疫试剂盒 ,英文名: ELISA Kit for Insulin Like Growth Factor 1 Receptor (IGF1R) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A16766 细胞外基质金属蛋白酶诱导因子(EMMPRIN)酶联免疫试剂盒 ,英文名: ELISA Kit for Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A16767 脂多糖结合蛋白(LBP)酶联免疫试剂盒 ,英文名: ELISA Kit for Lipopolysaccharide Binding Protein (LBP) ,适用种属: Sus scrofa; Porcine (Pig) ,规格: 48T/96T
A16768 胎球蛋白B(FETUB)酶联免疫试剂盒 ,英文名: ELISA Kit for Fetuin B (FETUB) ,适用种属: Bos taurus; Bovine (Cattle) ,规格: 48T/96T
A16769 白介素2受体α(IL2Rα)酶联免疫试剂盒 ,英文名: ELISA Kit for Interleukin 2 Receptor Alpha (IL2Ra) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A16770 血小板激活因子乙酰水解酶2(PAFAH2)酶联免疫试剂盒 ,英文名: ELISA Kit for Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A16771 淀粉样蛋白β前体蛋白结合蛋白B3(APBB3)酶联免疫试剂盒 ,英文名: ELISA Kit for Amyloid Beta Precursor Protein Binding Protein B3 (APBB3) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A16772 淀粉样蛋白β前体蛋白结合蛋白2(APPBP2)酶联免疫试剂盒 ,英文名: ELISA Kit for Amyloid Beta Precursor Protein Binding Protein 2 (APPBP2) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A16773 鞘氨醇1磷酸酯受体1(S1PR1)酶联免疫试剂盒 ,英文名: ELISA Kit for Sphingosine 1 Phosphate Receptor 1 (S1PR1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16774 90kDa热休克蛋白αA1(HSP90αA1)酶联免疫试剂盒 ,英文名: ELISA Kit for Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16775 90kDa热休克蛋白αB1(HSP90αB1)酶联免疫试剂盒 ,英文名: ELISA Kit for Heat Shock Protein 90kDa Alpha B1 (HSP90aB1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16776 90kDa热休克蛋白αB1(HSP90αB1)酶联免疫试剂盒 ,英文名: ELISA Kit for Heat Shock Protein 90kDa Alpha B1 (HSP90aB1) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A16777 可的松(COR)酶联免疫试剂盒 ,英文名: ELISA Kit for Cortisone (COR) ,适用种属: General ,规格: 48T/96T
A16778 和肽素(CPP)酶联免疫试剂盒 ,英文名: ELISA Kit for Copeptin (CPP) ,适用种属: Rattus norvegicus (Rat) ,规格: 48T/96T
A16779 X-射线修复交叉互补蛋白5(XRCC5)酶联免疫试剂盒 ,英文名: ELISA Kit for X-Ray Repair Cross Complementing 5 (XRCC5) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16780 X-射线修复交叉互补蛋白5(XRCC5)酶联免疫试剂盒 ,犬肾细胞系/IgR;MDCK/IgR英文名: ELISA Kit for X-Ray Repair Cross Complementing 5 (XRCC5) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T
A16781 T-细胞可诱导共刺激分子配体(ICOSLG)酶联免疫试剂盒 ,英文名: ELISA Kit for Inducible T-Cell Co Stimulator Ligand (ICOSLG) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16782 肌钙蛋白T(TNT)酶联免疫试剂盒 ,英文名: ELISA Kit for Troponin T (TNT) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A16783 Apelin蛋白(APLN)酶联免疫试剂盒 ,英文名: ELISA Kit for Apelin (APLN) ,适用种属: Mus musculus (Mouse) ,规格: 48T/96T