豹猫皮肤成纤维样细胞;LCS5
成纤维细胞样
贴壁生长
该细胞系来源于一雌性豹猫的皮肤组织。2004年由中国科学院昆明细胞库建立。
DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%FBS
1:2传代;3-4天一次
3
基础培养基+5%DMSO+20%FBS
公司细胞的简洁信息:
(1)储存:液氮。
(2)运输:干冰。
(3)用途:只可用于科研。
(4)第二代培养末期液氮冻存。
(5)豹猫皮肤成纤维样细胞;LCS5每冻存管细胞数:>5×105 cells/1ml。
(6)不含有HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌。
收到豹猫皮肤成纤维样细胞;LCS5后的处理:
1)收到细胞后,首先观察瓶身是否完好(注意有无缝隙,裂痕)。
2)显微镜下观察细胞的生长状况,有无细胞漂浮。
3)用新洁尔灭消毒瓶口及瓶身。
4)打开瓶口,再次用新洁尔灭消毒瓶口(可能会有液体溢出,注意不要碰到液体),酒精灯烤瓶口消毒。
5)将培养液转移至50ml离心管或广口瓶中(若细胞漂浮严重,离心培养基,1000g*5分钟,将离心后的培养基转移至另一个大离心管中,留一点吹打细胞沉淀成悬液,回收细胞至原培养瓶),若漂浮不严重,亦离心一下。
6)在原培养瓶中留一部分原装培养液,再补加自己新配的培养基至适当容积,例如4ml,让细胞适应一下环境。 当细胞长到70-80%,冻存大部分,小部分传代。
A09514天青杀素1(AZU1)酶联免疫试剂盒,英文名:ELISA Kit for Azurocidin 1 (AZU1),适用种属:Homo sapiens (Human),规格:48T/96T
A09515γ-促脂解激素(γLPH)酶联免疫试剂盒,英文名:ELISA Kit for Gamma-Lipotropic Hormone (gLPH),适用种属:Homo sapiens (Human),规格:48T/96T
A09516软骨糖蛋白39(GP39)酶联免疫试剂盒,英文名:ELISA Kit for Glycoprotein 39, Cartilage (GP39),适用种属:Homo sapiens (Human),规格:48T/96T
A09517溶酶体关联膜蛋白2(LAMP2)酶联免疫试剂盒,英文名:ELISA Kit for Lysosomal Associated Membrane Protein 2 (LAMP2),适用种属:Homo sapiens (Human),规格:48T/96T
A09518间隙连接蛋白37(CX37)酶联免疫试剂盒,英文名:ELISA Kit for Connexin 37 (CX37),适用种属:Homo sapiens (Human),规格:48T/96T
A09519间隙连接蛋白40(CX40)酶联免疫试剂盒,英文名:ELISA Kit for Connexin 40 (CX40),适用种属:Homo sapiens (Human),规格:48T/96T
A09520间隙连接蛋白31(CX31)酶联免疫试剂盒,英文名:ELISA Kit for Connexin 31 (CX31),适用种属:Homo sapiens (Human),规格:48T/96T
A09521甘露糖结合蛋白(MBL)酶联免疫试剂盒,英文名:ELISA Kit for Mannose Binding Lectin (MBL),适用种属:Homo sapiens (Human),规格:48T/96T
A09522神经钙黏蛋白(CDH2)酶联免疫试剂盒,英文名:ELISA Kit for Cadherin, Neuronal (CDH2),适用种属:Homo sapiens (Human),规格:48T/96T
A09523唾液淀粉酶α1(AMY1)酶联免疫试剂盒,英文名:ELISA Kit for Amylase Alpha 1, Salivary (AMY1),适用种属:Homo sapiens (Human),规格:48T/96T
A09524胰弹性蛋白酶(ELA1)酶联免疫试剂盒,英文名:ELISA Kit for Elastase 1, Pancreatic (ELA1),适用种属:Homo sapiens (Human),规格:48T/96T
A09525成骨生长肽(OGP)酶联免疫试剂盒,英文名:ELISA Kit for Osteogenic Growth Peptide (OGP),适用种属:Homo sapiens (Human),规格:48T/96T
A09526白介素1α(IL1α)酶联免疫试剂盒,英文名:ELISA Kit for Interleukin 1 Alpha (IL1a),适用种属:Sus scrofa; Porcine (Pig),规格:48T/96T
A09527硫氧化还原蛋白(Trx)酶联免疫试剂盒,英文名:ELISA Kit for Thioredoxin (Trx),适用种属:Homo sapiens (Human),规格:48T/96T
A09528硫氧化还原蛋白(Trx)酶联免疫试剂盒,英文名:ELISA Kit for Thioredoxin (Trx),适用种属:Rattus norvegicus (Rat),规格:48T/96T
A09529硫氧化还原蛋白(Trx)酶联免疫试剂盒,英文名:ELISA Kit for Thioredoxin (Trx),适用种属:Mus musculus (Mouse),规格:48T/96T
A09530硫氧还蛋白还原酶1(TrxR1)酶联免疫试剂盒,英文名:ELISA Kit for Thioredoxin Reductase 1 (TrxR1),适用种属:Homo sapiens (Human),规格:48T/96T
A09531硫氧还蛋白还原酶1(TrxR1)酶联免疫试剂盒,英文名:ELISA Kit for Thioredoxin Reductase 1 (TrxR1),适用种属:Rattus norvegicus (Rat),规格:48T/96T
A09532硫氧还蛋白还原酶1(TrxR1)酶联免疫试剂盒,英文名:ELISA Kit for Thioredoxin Reductase 1 (TrxR1),适用种属:Mus musculus (Mouse),规格:48T/96T
A09533孤啡肽(OFQ)酶联免疫试剂盒,英文名:ELISA Kit for Orphanin FQ (OFQ),适用种属:Homo sapiens (Human),规格:48T/96T
A09534孤啡肽(OFQ)酶联免疫试剂盒,英文名:ELISA Kit for Orphanin FQ (OFQ),适用种属:Rattus norvegicus (Rat),规格:48T/96T
A09535孤啡肽(OFQ)酶联免疫试剂盒,英文名:ELISA Kit for Orphanin FQ (OFQ),适用种属:Mus musculus (Mouse),规格:48T/96T
A09536基质金属蛋白酶1(MMP1)酶联免疫试剂盒,英文名:ELISA Kit for Matrix Metalloproteinase 1 (MMP1),适用种属:Oryctolagus cuniculus (Rabbit),规格:48T/96T
A09537内凝集蛋白1(ITLN1)酶联免疫试剂盒,英文名:ELISA Kit for Intelectin 1 (ITLN1),适用种属:Homo sapiens (Human),规格:48T/96T
A09538内凝集蛋白1(ITLN1)酶联免疫试剂盒,英文名:ELISA Kit for Intelectin 1 (ITLN1),适用种属:Mus musculus (Mouse),规格:48T/96T
A09539内凝集蛋白1(ITLN1)酶联免疫试剂盒,英文名:ELISA Kit for Intelectin 1 (ITLN1),适用种属:Rattus norvegicus (Rat),规格:48T/96T
A09540载脂蛋白E(APOE)酶联免疫试剂盒,英文名:ELISA Kit for Apolipoprotein E (APOE),适用种属:Homo sapiens (Human),规格:48T/96T
A09541载脂蛋白E(APOE)酶联免疫试剂盒,英文名:ELISA Kit for Apolipoprotein E (APOE),适用种属:Rattus norvegicus (Rat),规格:48T/96T
A09542载脂蛋白E(APOE)酶联免疫试剂盒,英文名:ELISA Kit for Apolipoprotein E (APOE),适用种属:Mus musculus (Mouse),规格:48T/96T
A09543过氧化物酶体增殖物激活受体α(PPARα)酶联免疫试剂盒,英文名:ELISA Kit for Peroxisome Proliferator Activated Receptor Alpha (PPARa),适用种属:Homo sapiens (Human),规格:48T/96T
A09544过氧化物酶体增殖物激活受体α(PPARα)酶联免疫试剂盒,英文名:ELISA Kit for Peroxisome Proliferator Activated Receptor Alpha (PPARa),适用种属:Rattus norvegicus (Rat),规格:48T/96T
A09545过氧化物酶体增殖物激活受体α(PPARα)酶联免疫试剂盒,英文名:ELISA Kit for Peroxisome Proliferator Activated Receptor Alpha (PPARa),适用种属:Mus musculus (Mouse),规格:48T/96T
A09546粘蛋白2(MUC2)酶联免疫试剂盒,英文名:ELISA Kit for Mucin 2 (MUC2),适用种属:Homo sapiens (Human),规格:48T/96T
A09547C反应蛋白(CRP)酶联免疫试剂盒,英文名:ELISA Kit for C Reactive Protein (CRP),适用种属:Oryctolagus cuniculus (Rabbit),规格:48T/96T
A09548白三烯D4(LTD4)酶联免疫试剂盒,英文名:ELISA Kit for Leukotriene D4 (LTD4),适用种属:General,规格:48T/96T
A09549单核细胞趋化蛋白4(MCP4)酶联免疫试剂盒,英文名:ELISA Kit for Monocyte Chemotactic Protein 4 (MCP4),适用种属:Homo sapiens (Human),规格:48T/96T
A09550尿激酶型纤溶酶原激活因子(uPA)酶联免疫试剂盒,英文名:ELISA Kit for Plasminogen Activator, Urokinase (uPA),适用种属:Oryctolagus cuniculus (Rabbit),规格:48T/96T
A09551内脏脂肪特异性丝氨酸蛋白酶抑制因子(Vaspin)酶联免疫试剂盒,英文名:ELISA Kit for Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin),适用种属:Homo sapiens (Human),规格:48T/96T
A09552Preptin蛋白(Preptin)酶联免疫试剂盒,英文名:ELISA Kit for Preptin,适用种属:Homo sapiens (Human),规格:48T/96T
A09553α1-微球蛋白(α1M)酶联免疫试剂盒,英文名:ELISA Kit for Alpha-1-Microglobulin/Bikunin Precursor (a1M),适用种属:Homo sapiens (Human),规格:48T/96T
A09554α1-微球蛋白(α1M)酶联免疫试剂盒,英文名:ELISA Kit for Alpha-1-Microglobulin/Bikunin Precursor (a1M),适用种属:Rattus norvegicus (Rat),规格:48T/96T
A09555α1-微球蛋白(α1M)酶联免疫试剂盒,英文名:ELISA Kit for Alpha-1-Microglobulin/Bikunin Precursor (a1M),适用种属:Mus musculus (Mouse),规格:48T/96T
A09556钙调素(CAM)酶联免疫试剂盒,英文名:ELISA Kit for Calmodulin (CAM),适用种属:Homo sapiens (Human),规格:48T/96T
A09557内皮型一氧化氮合酶(NOS3)酶联免疫试剂盒,英文名:ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3),适用种属:Oryctolagus cuniculus (Rabbit),规格:48T/96T
A09558细胞色素P450家族成员2E1(CYP2E1)酶联免疫试剂盒,英文名:ELISA Kit for Cytochrome P450 2E1 (CYP2E1),适用种属:Rattus norvegicus (Rat),规格:48T/96T
A09559转化生长因子β2(TGFβ2)酶联免疫试剂盒,豹猫皮肤成纤维样细胞;LCS5英文名:ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2),适用种属:Homo sapiens (Human),规格:48T/96T
A09560转化生长因子β2(TGFβ2)酶联免疫试剂盒,英文名:ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2),适用种属:Rattus norvegicus (Rat),规格:48T/96T
A09561转化生长因子β2(TGFβ2)酶联免疫试剂盒,英文名:ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2),适用种属:Mus musculus (Mouse),规格:48T/96T
A09562转化生长因子β2(TGFβ2)酶联免疫试剂盒,英文名:ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2),适用种属:Oryctolagus cuniculus (Rabbit),规格:48T/96T