人乳腺癌高转移细胞亚系;MDA-MB-231sci
上皮细胞样
贴壁生长
该细胞属专利保藏,其特征特性尚未公开。
MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts) 10%FBS
1:3传代,3-4天传1次
C2
基础培养基+5%DMSO+20%FBS
公司细胞的简洁信息:
(1)储存:液氮。
(2)运输:干冰。
(3)用途:只可用于科研。
(4)第二代培养末期液氮冻存。
(5)人乳腺癌高转移细胞亚系;MDA-MB-231sci冻存管细胞数:>5×105 cells/1ml。
(6)不含有HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌。
收到人乳腺癌高转移细胞亚系;MDA-MB-231sci后的处理:
1)收到细胞后,首先观察瓶身是否完好(注意有无缝隙,裂痕)。
2)显微镜下观察细胞的生长状况,有无细胞漂浮。
3)用新洁尔灭消毒瓶口及瓶身。
4)打开瓶口,再次用新洁尔灭消毒瓶口(可能会有液体溢出,注意不要碰到液体),酒精灯烤瓶口消毒。
5)将培养液转移至50ml离心管或广口瓶中(若细胞漂浮严重,离心培养基,1000g*5分钟,将离心后的培养基转移至另一个大离心管中,留一点吹打细胞沉淀成悬液,回收细胞至原培养瓶),若漂浮不严重,亦离心一下。
6)在原培养瓶中留一部分原装培养液,再补加自己新配的培养基至适当容积,例如4ml,让细胞适应一下环境。 当细胞长到70-80%,冻存大部分,小部分传代。
XR-E-199126 猪凝血因子ⅩⅢ(FⅩⅢ)酶联免疫吸附测定试剂盒 ,英文名: Pig coagulation factor ⅩⅢ,FⅩⅢ ELISA Kit ,规格: 48T/96T
XR-E-199127 猪凝血因子X(F10)酶联免疫吸附测定试剂盒 ,英文名: Pig coagulation factor X,F10 ELISA kit ,规格: 48T/96T
XR-E-199128 猪凝血因子XIIIA链(F13A1)酶联免疫吸附测定试剂盒 ,英文名: Pig Coagulation factor XIII A chain,F13A1 ELISA kit ,规格: 48T/96T
XR-E-199129 猪凝血因子XIIIB链(F13B)酶联免疫吸附测定试剂盒 ,英文名: Pig Coagulation factor XIII B chain,F13B ELISA kit ,规格: 48T/96T
XR-E-199130 猪皮质类固醇结合球蛋白(SERPINA6)酶联免疫吸附测定试剂盒 ,英文名: Pig Corticosteroid-binding globulin,SERPINA6 ELISA kit ,规格: 48T/96T
XR-E-199131 猪皮质酮(CORT)酶联免疫吸附测定试剂盒 ,英文名: Porcine Corticosterone,CORT ELISA Kit ,规格: 48T/96T
XR-E-199132 猪皮质抑素/皮质醇稳定蛋白(CORT)酶联免疫吸附测定试剂盒 ,英文名: Pig cortistatin,CORT ELISA kit ,规格: 48T/96T
XR-E-199133 猪葡萄糖转运蛋白2(GLUT2)酶联免疫吸附测定试剂盒 ,英文名: Pig Glucose transporter 2,GLUT2 ELISA Kit ,规格: 48T/96T
XR-E-199134 猪前列腺素F2α(PGF2α)酶联免疫吸附测定试剂盒 ,英文名: Pig Prostaglandin F2α,PGF2α ELISA Kit ,规格: 48T/96T
XR-E-199135 猪前列腺素G/H合酶2(PTGS2)酶联免疫吸附测定试剂盒 ,英文名: Pig prostaglandin G/H synthase 2,PTGS2 ELISA kit ,规格: 48T/96T
XR-E-199136 猪趋化因子配体28(CCL28)酶联免疫吸附测定试剂盒 ,英文名: Pig chemokine(C-C motif)ligand 28(CCL28)ELISA kit ,规格: 48T/96T
XR-E-199137 猪去乙酰化饥饿激素(dGHRL)酶联免疫吸附测定试剂盒 ,英文名: Pig deacylated appetite-regulating hormone,dGHRL ELISA kit ,规格: 48T/96T
XR-E-199138 猪热休克蛋白60(HSPD1/Hsp-60)酶联免疫吸附测定试剂盒 ,英文名: Pig 60 kDa heat shock protein,mitochondrial(HSPD1)ELISA kit ,规格: 48T/96T
XR-E-199139 猪热休克蛋白70kDa蛋白1A(HSPA1A)酶联免疫吸附测定试剂盒 ,英文名: Pig Heat shock 70 kDa protein 1A,HSPA1A ELISA kit ,规格: 48T/96T
XR-E-199140 猪乳酸脱氢酶(LDH)酶联免疫吸附测定试剂盒 ,英文名: Pig lactate dehydrogenase,LDH ELISA Kit ,规格: 48T/96T
XR-E-199141 猪乳铁传递蛋白/乳铁蛋白(LF/LTF)酶联免疫吸附测定试剂盒 ,英文名: Pig Lactoferrin,LF/LTF ELISA kit ,规格: 48T/96T
XR-E-199142 猪三碘甲状腺原氨酸(T3)酶联免疫吸附测定试剂盒 ,英文名: Pig Tri-iodothyronine,T3 ELISA Kit ,规格: 48T/96T
XR-E-199143 猪色素上皮衍生因子(PEDF)酶联免疫吸附测定试剂盒 ,英文名: Pig pigment epithelium-derived factor,PEDF ELISA Kit ,规格: 48T/96T
XR-E-199144 猪神经胶质纤维酸性蛋白(GFAP)酶联免疫吸附测定试剂盒 ,英文名: Pig glial fibrillary acidic protein,GFAP ELISA Kit ,规格: 48T/96T
XR-E-199145 猪神经特异性烯醇化酶(NSE)酶联免疫吸附测定试剂盒 ,英文名: Pig Neuron-specific enolase,NSE ELISA Kit ,规格: 48T/96T
XR-E-199146 猪神经型一氧化氮合成酶(nNOS)酶联免疫吸附测定试剂盒 ,英文名: Pig Neuronal Nitric Oxide Synthase,nNOS ELISA Kit ,规格: 48T/96T
XR-E-199147 猪肾素(Renin)酶联免疫吸附测定试剂盒 ,英文名: Pig Renin ELISA Kit ,规格: 48T/96T
XR-E-199148 猪生长分化因子9(GDF9)酶联免疫吸附测定试剂盒 ,英文名: Pig Growth/differentiation factor 9,GDF9 ELISA kit ,规格: 48T/96T
XR-E-199149 猪生长激素受体(GHR)酶联免疫吸附测定试剂盒 ,英文名: Pig growth hormone receptor,GHR ELISA Kit ,规格: 48T/96T
XR-E-199150 猪生长激素抑制素受体5(SSTR5)酶联免疫吸附测定试剂盒 ,英文名: Pig Somatostatin receptor type 5,SSTR5 ELISA kit ,规格: 48T/96T
XR-E-199151 猪生长抑素受体5(SSR5)酶联免疫吸附测定试剂盒 ,英文名: Pig Somatostatin receptor 5,SSR5 ELISA Kit ,规格: 48T/96T
XR-E-199152 猪输血传播病毒/辛型肝炎病毒抗体(IgG)酶联免疫吸附测定试剂盒 ,英文名: Pig transfusion transimitted virus/Hepatitis H virus antibody(IgG)ELISA Kit ,规格: 48T/96T
XR-E-199153 猪双氢睾酮(DHT)酶联免疫吸附测定试剂盒 ,英文名: Pig Dihydrotestosterone,DHT ELISA Kit ,规格: 48T/96T
XR-E-199154 猪水通道蛋白1(AQP-1)酶联免疫吸附测定试剂盒 ,英文名: Pig Aquaporin 1,AQP-1 ELISA Kit ,规格: 48T/96T
XR-E-199155 猪水通道蛋白4(AQP-4)酶联免疫吸附测定试剂盒 ,英文名: Pig Aquaporin 4,AQP-4 ELISA Kit ,规格: 48T/96T
XR-E-199156 猪胎盘生长因子(PGF)酶联免疫吸附测定试剂盒 ,英文名: Pig Placenta growth factor,PGF ELISA kit ,规格: 48T/96T
XR-E-199157 猪胎球蛋白A(FetuinA)酶联免疫吸附测定试剂盒 ,英文名: Pig Fetuin A ELISA Kit ,规格: 48T/96T
XR-E-199158 猪糖化血红蛋白A1c(GHbA1c)酶联免疫吸附测定试剂盒 ,英文名: Pig Glycated hemoglobin A1c,GHbA1c ELISA Kit ,规格: 48T/96T
XR-E-199159 猪铁蛋白(FE)酶联免疫吸附测定试剂盒 ,英文名: Pig ferritin,FE ELISA Kit ,规格: 48T/96T
XR-E-199160 猪维生素D(VD)酶联免疫吸附测定试剂盒 ,英文名: Pig Vitamin D,VD ELISA kit ,规格: 48T/96T
XR-E-199161 猪伪狂犬病毒(PRV)抗体(IgG)酶联免疫吸附测定试剂盒 ,英文名: Pig pseudorabies virus(PRV)antibody(IgG)ELISA kit ,规格: 48T/96T
XR-E-199162 猪胃泌酸调节素(OXM)酶联免疫吸附测定试剂盒 ,英文名: Pig oxyntomodulin,OXM ELISA kit ,规格: 48T/96T
XR-E-199163 猪瘟抗体(IgG)酶联免疫吸附测定试剂盒 ,英文名: Classical Swine Fever Virus Antibody IgG ELISA Kit ,规格: 48T/96T
XR-E-199164 猪瘟抗体(IgM)酶联免疫吸附测定试剂盒 ,英文名: Pig Anti-CSFV Antibody IgM ELISA Kit ,规格: 48T/96T
XR-E-199165 猪瘟抗体(CSFV)酶联免疫吸附测定试剂盒 ,英文名: Classical Swine Fever Virus Antibody ELISA Kit ,规格: 48T/96T
XR-E-199166 猪戊糖素(PE)酶联免疫吸附测定试剂盒 ,英文名: Pig pentosidine,PE ELISA kit ,规格: 48T/96T
XR-E-199167 猪细胞间粘附分子2(ICAM-2)酶联免疫吸附测定试剂盒 ,英文名: Porcine intercellular adhesion molecule 2,ICAM-2 ELISA Kit ,规格: 48T/96T
XR-E-199168 猪细胞色素c还原酶(CCR)酶联免疫吸附测定试剂盒 ,英文名: Pig cytochrome c reductase,CCR ELISA kit ,规格: 48T/96T
XR-E-199169 猪细胞色素C氧化酶(COX)酶联免疫吸附测定试剂盒 ,英文名: Pig cytochrome C oxidase,COX ELISA kit ,规格: 48T/96T
XR-E-199170 猪酰基辅酶A去饱和酶(SCD)酶联免疫吸附测定试剂盒 ,英文名: Pig Acyl-CoA desaturase,SCD ELISA kit ,规格: 48T/96T
XR-E-199171 猪心肌特异性肌钙蛋白T(cTnT)酶联免疫吸附测定试剂盒 ,英文名: Pig cardiac isoform of Tropnin T,cTnT ELISA Kit ,规格: 48T/96T
XR-E-199172 猪心钠肽(ANP)酶联免疫吸附测定试剂盒 ,英文名: Pig Atrial Natriuretic Peptide,ANP ELISA Kit ,规格: 48T/96T
XR-E-199173 猪心型脂肪酸结合蛋白(FABP3/FABP11/MDGI)酶联免疫吸附测定试剂盒 ,英文名: Pig Fatty acid-binding protein, heart,FABP3 ELISA kit ,规格: 48T/96T
XR-E-199174 猪胸腺肽β4(Thymosin β4)酶联免疫吸附测定试剂盒 ,英文名: Pig Thymosin β4 ELISA Kit ,规格: 48T/96T
XR-E-199175 猪旋毛虫IgG抗体(Trichinella IgG Ab)酶联免疫吸附测定试剂盒 ,英文名: Pig Trichinella IgG antibody ELISA Kit ,规格: 48T/96T
XR-E-199176 猪血管活性肠肽(VIP)酶联免疫吸附测定试剂盒 ,英文名: Pig Vasoactive Intestinal Peptide,VIP ELISA Kit ,规格: 48T/96T
XR-E-199177 猪血管紧张素Ⅱ(ANG-Ⅱ)酶联免疫吸附测定试剂盒 ,英文名: Pig angiotension Ⅱ,ANG-Ⅱ ELISA Kit ,规格: 48T/96T
XR-E-199178 猪血管紧张素转化酶(ACE)酶联免疫吸附测定试剂盒 ,英文名: Pig Angiotensin Converting Enzyme,ACE ELISA Kit ,规格: 48T/96T
XR-E-199179 猪血管紧张素转化酶2(ACE2)酶联免疫吸附测定试剂盒 ,英文名: Pig Angiotensin converting enzyme 2,ACE2 ELISA Kit ,人乳腺癌高转移细胞亚系;MDA-MB-231sci规格: 48T/96T
XR-E-199180 猪血管内皮细胞生长因子(VEGF)酶联免疫吸附测定试剂盒 ,英文名: Pig Vascular Endothelial cell Growth Factor,VEGF ELISA KIT ,规格: 48T/96T
XR-E-199181 猪血管内皮细胞粘附分子1(VCAM-1)酶联免疫吸附测定试剂盒 ,英文名: Porcine Vascular cell adhesion molecule 1,VCAM-1 ELISA Kit ,规格: 48T/96T
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