猪肾传代细胞;PK15-B1
上皮细胞样
贴壁生长
该细胞属专利保藏,其特征特性尚未公开。
MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts) 10%FBS
1:3传代,3-4天传1次
C5
基础培养基+5%DMSO+20%FBS
公司细胞的简洁信息:
(1)储存:液氮。
(2)运输:干冰。
(3)用途:只可用于科研。
(4)第二代培养末期液氮冻存。
(5)猪肾传代细胞;PK15-B1冻存管细胞数:>5×105 cells/1ml。
(6)不含有HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌。
收到猪肾传代细胞;PK15-B1后的处理:
1)收到细胞后,首先观察瓶身是否完好(注意有无缝隙,裂痕)。
2)显微镜下观察细胞的生长状况,有无细胞漂浮。
3)用新洁尔灭消毒瓶口及瓶身。
4)打开瓶口,再次用新洁尔灭消毒瓶口(可能会有液体溢出,注意不要碰到液体),酒精灯烤瓶口消毒。
5)将培养液转移至50ml离心管或广口瓶中(若细胞漂浮严重,离心培养基,1000g*5分钟,将离心后的培养基转移至另一个大离心管中,留一点吹打细胞沉淀成悬液,回收细胞至原培养瓶),若漂浮不严重,亦离心一下。
6)在原培养瓶中留一部分原装培养液,再补加自己新配的培养基至适当容积,例如4ml,让细胞适应一下环境。 当细胞长到70-80%,冻存大部分,小部分传代。
XR-E-198500 小鼠α1干扰素(IFN-α1)酶联免疫吸附测定试剂盒 ,英文名: Mouse Interferon α1,IFN-α1 ELISA Kit ,规格: 48T/96T
XR-E-198501 小鼠α1抗胰糜蛋白酶(AACT)酶联免疫吸附测定试剂盒 ,英文名: Mouse Alpha1 Antichymotrypsin,AACT ELISA Kit ,规格: 48T/96T
XR-E-198502 小鼠α1微球蛋白(α1-MG)酶联免疫吸附测定试剂盒 ,英文名: Mouse α1-microglobulin,α1-MG ELISA Kit ,规格: 48T/96T
XR-E-198503 小鼠α谷胱甘肽S转移酶(α-GST)酶联免疫吸附测定试剂盒 ,英文名: Mouse α-glutathione S-transferases,α-GST ELISA Kit ,规格: 48T/96T
XR-E-198504 小鼠α-黑色素细胞刺激素(α-MSH)酶联免疫吸附测定试剂盒 ,英文名: Mouse α-Melanocyte Stimulating Hormone,α-MSH ELISA Kit ,规格: 48T/96T
XR-E-198505 小鼠α葡萄糖苷酶(α-glucosidase)酶联免疫吸附测定试剂盒 ,英文名: Mouse α-Glucosidase ELISA Kit ,规格: 48T/96T
XR-E-198506 小鼠β1肾上腺素能受体(β1AR)抗体酶联免疫吸附测定试剂盒 ,英文名: Mouse anti-β1-adrenergic receptor(β1AR)antibody ELISA kit ,规格: 48T/96T
XR-E-198507 小鼠β2微球蛋白(BMG/β2-MG)酶联免疫吸附测定试剂盒 ,英文名: Mouse β2-microglobulin,BMG/β2-MG ELISA Kit ,规格: 48T/96T
XR-E-198508 小鼠β氨基己糖苷酶A(β-Hex A)酶联免疫吸附测定试剂盒 ,英文名: Mouse β-hexosaminidase A,β-Hex A ELISA Kit ,规格: 48T/96T
XR-E-198509 小鼠β-防御素1(β-BD-1)酶联免疫吸附测定试剂盒 ,英文名: Mouse β-Defensins 1 ELISA Kit ,规格: 48T/96T
XR-E-198510 小鼠β-防御素3(β-BD-3)酶联免疫吸附测定试剂盒 ,英文名: Mouse β-defensins 3 ELISA Kit ,规格: 48T/96T
XR-E-198511 小鼠β干扰素(IFN-β/IFNB)酶联免疫吸附测定试剂盒 ,英文名: Mouse Interferon β,IFN-β/IFNB ELISA Kit ,规格: 48T/96T
XR-E-198512 小鼠β连环蛋白/联蛋白(β-Cat)酶联免疫吸附测定试剂盒 ,英文名: Mouse β-catenin,β-cat ELISA Kit ,规格: 48T/96T
XR-E-198513 小鼠β内啡肽受体(β-EPR)酶联免疫吸附测定试剂盒 ,英文名: Mouse Beta-Endorphin receptor,β-EPR ELISA Kit ,规格: 48T/96T
XR-E-198514 小鼠β萘酚(β-naphthol)酶联免疫吸附测定试剂盒 ,英文名: Mouse Beta-naphthol ELISA Kit ,规格: 48T/96T
XR-E-198515 小鼠β葡萄糖醛酸苷酶(βGD)酶联免疫吸附测定试剂盒 ,英文名: Mouse β-glucuronidase,βGD ELISA Kit ,规格: 48T/96T
XR-E-198516 小鼠β位淀粉样前体蛋白裂解酶1(BACE1)酶联免疫吸附测定试剂盒 ,英文名: Mouse β-site APP-Cleaving Enzyme 1,BACE1 ELISA Kit ,规格: 48T/96T
XR-E-198517 小鼠β细胞素(BTC)酶联免疫吸附测定试剂盒 ,英文名: Mouse beta cellulin,BTC ELISA Kit ,规格: 48T/96T
XR-E-198518 小鼠β血小板球蛋白/β血栓环蛋白(β-TG)酶联免疫吸附测定试剂盒 ,英文名: Mouse β-Thromboglobulin,β-TG ELISA Kit ,规格: 48T/96T
XR-E-198519 小鼠γ氨基丁酸(GABA)自身抗体IgG 酶联免疫吸附测定试剂盒 ,英文名: Mouse Gamma-Aminobutyric Acid(GABA)autoantibody IgG ELISA Kit ,规格: 48T/96T
XR-E-198520 小鼠γ干扰素受体(IFN-γR)酶联免疫吸附测定试剂盒 ,英文名: Mouse Interferon γ Receptor,IFN-γR ELISA Kit ,规格: 48T/96T
XR-E-198521 小鼠γ突触核蛋白(SNCG)酶联免疫吸附测定试剂盒 ,英文名: Mouse Synuclein-gamma,SNCG ELISA Kit ,规格: 48T/96T
XR-E-198522 小鼠阿立新A(Orexin A)酶联免疫吸附测定试剂盒 ,英文名: Mouse Orexin A ELISA Kit ,规格: 48T/96T
XR-E-198523 小鼠癌胚抗原(CEA)酶联免疫吸附测定试剂盒 ,英文名: Mouse carcinoembryonic antign,CEA ELISA Kit ,规格: 48T/96T
XR-E-198524 小鼠铵氧化酶(含黄素)A(MAOA)酶联免疫吸附测定试剂盒 ,英文名: Mouse Amine oxidase [flavin-containing] A(MAOA) ELISA kit ,规格: 48T/96T
XR-E-198525 小鼠白蛋白(albumin)酶联免疫吸附测定试剂盒 ,英文名: Mouse albumin ELISA Kit ,规格: 48T/96T
XR-E-198526 小鼠白喉IgG抗体酶联免疫吸附测定试剂盒 ,英文名: Mouse Diphtheria IgG antibody ELISA Kit ,规格: 48T/96T
XR-E-198527 小鼠白介素18结合蛋白(IL-18BP)酶联免疫吸附测定试剂盒 ,英文名: Mouse interleukin-18 binding prorein,IL-18BP ELISA Kit ,规格: 48T/96T
XR-E-198528 小鼠白介素1β前体(pro-IL1B)酶联免疫吸附测定试剂盒 ,英文名: Mouse pro interleukin 1 beta,pro-IL1B ELISA Kit ,规格: 48T/96T
XR-E-198529 小鼠白介素1可溶性受体Ⅰ(IL-1sRⅠ)酶联免疫吸附测定试剂盒 ,英文名: Mouse soluble interleukin-1 receptor Ⅰ,IL-1sRⅠ ELISA kit ,规格: 48T/96T
XR-E-198530 小鼠白介素1可溶性受体Ⅱ(IL-1sRⅡ)酶联免疫吸附测定试剂盒 ,英文名: Mouse soluble interleukin-1 receptor Ⅱ,IL-1sRⅡ ELISA kit ,规格: 48T/96T
XR-E-198531 小鼠白介素1受体拮抗剂(IL1Ra/CD121)酶联免疫吸附测定试剂盒 ,英文名: Mouse Interleukin 1 receptor antagonist,IL-1ra ELISA kit ,规格: 48T/96T
XR-E-198532 小鼠白介素1受体相关激酶1(IRAK1)酶联免疫吸附测定试剂盒 ,英文名: Mouse Interleukin-1 receptor-associated kinase 1,IRAK1 ELISA kit ,规格: 48T/96T
XR-E-198533 小鼠白介素22(IL-22)酶联免疫吸附测定试剂盒 ,英文名: Mouse Interleukin 22,IL-22 ELISA Kit ,规格: 48T/96T
XR-E-198534 小鼠白介素25(IL25/C19orf10)酶联免疫吸附测定试剂盒 ,英文名: Mouse interleukin 25,IL25/C19orf10 ELISA kit ,规格: 48T/96T
XR-E-198535 小鼠白介素28(IL-28)酶联免疫吸附测定试剂盒 ,英文名: Mouse Interleukin 28,IL-28 ELISA Kit ,规格: 48T/96T
XR-E-198536 小鼠白介素28A(IL-28A)酶联免疫吸附测定试剂盒 ,英文名: Mouse Interleukin 28A,IL-28A ELISA Kit ,规格: 48T/96T
XR-E-198537 小鼠白介素29(IL-29)酶联免疫吸附测定试剂盒 ,英文名: Mouse Interleukin 29,IL-29 ELISA Kit ,规格: 48T/96T
XR-E-198538 小鼠白介素2可溶性受体α链(sIL-2Rα/CD25)酶联免疫吸附测定试剂盒 ,英文名: Mouse soluble interleukin-2 receptor,sIL-2Rα ELISA kit ,规格: 48T/96T
XR-E-198539 小鼠白介素2受体β(IL2rb/CD122)酶联免疫吸附测定试剂盒 ,英文名: Mouse Interleukin-2 receptor subunit beta,Il2rb/CD122 ELISA Kit ,规格: 48T/96T
XR-E-198540 小鼠白介素31(IL-31)酶联免疫吸附测定试剂盒 ,英文名: Mouse Interleukin 31,IL-31 ELISA Kit ,规格: 48T/96T
XR-E-198541 小鼠白介素33(IL-33)酶联免疫吸附测定试剂盒 ,英文名: Mouse Interleukin 33,IL-33 ELISA Kit ,规格: 48T/96T
XR-E-198542 小鼠白介素35(IL-35)酶联免疫吸附测定试剂盒 ,英文名: Mouse Interleukin 35,IL-35 ELISA Kit ,规格: 48T/96T
XR-E-198543 小鼠白三烯E4(LTE4)酶联免疫吸附测定试剂盒 ,英文名: Mouse leukotriene E4,LT-E4 ELISA Kit ,规格: 48T/96T
XR-E-198544 小鼠白细胞活化黏附因子(ALCAM)酶联免疫吸附测定试剂盒 ,英文名: Mouse Activated Leukocyte Cell Adhesion Molecule,ALCAM ELISA Kit ,规格: 48T/96T
XR-E-198545 小鼠百白破抗体(DPT)酶联免疫吸附测定试剂盒 ,英文名: Mouse DPT antibody ELISA Kit ,规格: 48T/96T
XR-E-198546 小鼠百日咳病毒IgG抗体酶联免疫吸附测定试剂盒 ,英文名: Mouse bordetella pertussis IgG antibody ELISA Kit ,规格: 48T/96T
XR-E-198547 小鼠半乳糖6硫酸酯酶(Gal-6S)酶联免疫吸附测定试剂盒 ,英文名: Mouse galactose-6-sulphate sulphatase,Gal-6S ELISA Kit ,规格: 48T/96T
XR-E-198548 小鼠半乳糖凝集素-3(Gal-3)酶联免疫吸附测定试剂盒 ,英文名: Mouse Galectin 3,GAL-3 ELISA Kit ,猪肾传代细胞;PK15-B1规格: 48T/96T
XR-E-198549 小鼠膀胱肿瘤抗原(BTA)酶联免疫吸附测定试剂盒 ,英文名: Mouse Bladder tumor antigen,BTA ELISA Kit ,规格: 48T/96T
XR-E-198550 小鼠胞浆型磷脂酶A2(cPLA2)酶联免疫吸附测定试剂盒 ,英文名: Mouse cytosolic phospholipase A2,cPLA2 ELISA Kit ,规格: 48T/96T
XR-E-198551 小鼠胞内氯离子通道蛋白1(CLIC1)酶联免疫吸附测定试剂盒 ,英文名: Mouse Chloride intracellular channel protein 1,CLIC1 ELISA kit ,规格: 48T/96T