杂交瘤细胞;HTK
淋巴母细胞样
半贴壁生长
该细胞属专利保藏,其特征特性尚未公开。
RPMI 1640 (w/o Hepes) 10%FBS
1:3传代,3-4天传1次
C2
基础培养基+5%DMSO+20%FBS
公司细胞的简洁信息:
(1)储存:液氮。
(2)运输:干冰。
(3)用途:只可用于科研。
(4)第二代培养末期液氮冻存。
(5)杂交瘤细胞;HTK冻存管细胞数:>5×105 cells/1ml。
(6)不含有HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌。
收到杂交瘤细胞;HTK后的处理:
1)收到细胞后,首先观察瓶身是否完好(注意有无缝隙,裂痕)。
2)显微镜下观察细胞的生长状况,有无细胞漂浮。
3)用新洁尔灭消毒瓶口及瓶身。
4)打开瓶口,再次用新洁尔灭消毒瓶口(可能会有液体溢出,注意不要碰到液体),酒精灯烤瓶口消毒。
5)将培养液转移至50ml离心管或广口瓶中(若细胞漂浮严重,离心培养基,1000g*5分钟,将离心后的培养基转移至另一个大离心管中,留一点吹打细胞沉淀成悬液,回收细胞至原培养瓶),若漂浮不严重,亦离心一下。
6)在原培养瓶中留一部分原装培养液,再补加自己新配的培养基至适当容积,例如4ml,让细胞适应一下环境。 当细胞长到70-80%,冻存大部分,小部分传代。
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XR-E-198406 小鼠1,3-βD葡葡糖苷酶(1,3-βD-Glu)酶联免疫吸附测定试剂盒 ,英文名: Mouse 1,3-βD-Glu ELISA Kit ,规格: 48T/96T
XR-E-198407 小鼠1,4,5-三磷酸肌醇(IP3)酶联免疫吸附测定试剂盒 ,英文名: Mouse inositol 1,4,5,-trisphosphate,IP3 ELISA Kit ,规格: 48T/96T
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XR-E-198409 小鼠17-α甲睾酮(17-αMT)酶联免疫吸附测定试剂盒 ,英文名: Mouse 17-alpha Methyltestosterone,17-αMT ELISA Kit ,规格: 48T/96T
XR-E-198410 小鼠17-酮类固醇(17-KS)酶联免疫吸附测定试剂盒 ,英文名: Mouse 17-ketosteroids,17-KS ELISA Kit ,规格: 48T/96T
XR-E-198411 小鼠Ⅰ型胶原C端肽(CTX-Ⅰ)酶联免疫吸附测定试剂盒 ,英文名: Mouse cross linked C-telopeptide of type I collagen,CTX-Ⅰ ELISA Kit ,规格: 48T/96T
XR-E-198412 小鼠Ⅰ型胶原N末端肽(NTX)酶联免疫吸附测定试剂盒 ,英文名: Mouse cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit ,规格: 48T/96T
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XR-E-198419 小鼠3-O-甲基多巴(3-OMD)酶联免疫吸附测定试剂盒 ,英文名: Mouse 3-O-methyldopa,3-OMD ELISA kit ,规格: 48T/96T
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XR-E-198423 小鼠Ⅲ型前胶原(HPCⅢ)酶联免疫吸附测定试剂盒 ,英文名: Mouse procollagen type III,HPCⅢ ELISA Kit ,规格: 48T/96T
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XR-E-198425 小鼠Ⅲ型前胶原肽(PⅢP)酶联免疫吸附测定试剂盒 ,英文名: Mouse procollagen Ⅲ propeptide,PⅢP ELISA Kit ,规格: 48T/96T
XR-E-198426 小鼠4-羟基壬烯酸(HNE)酶联免疫吸附测定试剂盒 ,英文名: Mouse 4-Hydroxynonenal,HNE ELISA Kit ,规格: 48T/96T
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XR-E-198435 小鼠Bcl-2相关X蛋白(BAX)酶联免疫吸附测定试剂盒 ,英文名: Mouse Bcl-2 associated X protein, Bax ELISA Kit ,规格: 48T/96T
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XR-E-198437 小鼠B细胞淋巴瘤-XL(Bcl-xl)酶联免疫吸附测定试剂盒 ,英文名: Mouse B-cell lymphoma-extra large,Bcl-xl ELISA kit ,规格: 48T/96T
XR-E-198438 小鼠Ca-ATP酶(Ca-ATPase)酶联免疫吸附测定试剂盒 ,英文名: Mouse Calcium ATPase,Ca-ATPase ELISA Kit ,规格: 48T/96T
XR-E-198439 小鼠cAMP反应元件结合蛋白(CREB)酶联免疫吸附测定试剂盒 ,英文名: Mouse cAMP response element binding protein,CREB ELISA Kit ,规格: 48T/96T
XR-E-198440 小鼠CC趋化因子受体6(CCR-6)酶联免疫吸附测定试剂盒 ,英文名: Mouse C-C Chemokine receptor 6,CCR-6 ELISA Kit ,规格: 48T/96T
XR-E-198441 小鼠CD19分子(CD19)酶联免疫吸附测定试剂盒 ,英文名: Mouse cluster of differentiation 19,CD19 ELISA Kit ,规格: 48T/96T
XR-E-198442 小鼠CD33分子(CD33)酶联免疫吸附测定试剂盒 ,英文名: Mouse cluster Of differentiation,CD33 ELISA Kit ,规格: 48T/96T
XR-E-198443 小鼠CD34分子(CD34)酶联免疫吸附测定试剂盒 ,英文名: Mouse cluster of differentiation 34,CD34 ELISA Kit ,规格: 48T/96T
XR-E-198444 小鼠CD44分子(CD44)酶联免疫吸附测定试剂盒 ,英文名: Mouse cluster of differentiation,CD44 ELISA Kit ,规格: 48T/96T
XR-E-198445 小鼠CD45分子(CD45)酶联免疫吸附测定试剂盒 ,英文名: Mouse cluster of differentiation 45,CD45 ELISA Kit ,规格: 48T/96T
XR-E-198446 小鼠CD4分子(CD4)酶联免疫吸附测定试剂盒 ,杂交瘤细胞;HTK英文名: Mouse T-cell surface glycoprotein CD4,CD4 ELISA kit ,规格: 48T/96T
XR-E-198447 小鼠CD8分子(CD8)酶联免疫吸附测定试剂盒 ,英文名: Mouse cluster of differentiation 8,CD8 ELISA Kit ,规格: 48T/96T
XR-E-198448 小鼠c-fos 酶联免疫吸附测定试剂盒 ,英文名: Mouse c-fos ELISA Kit ,规格: 48T/96T
XR-E-198449 小鼠c-jun 酶联免疫吸附测定试剂盒 ,英文名: Mouse c-jun ELISA Kit ,规格: 48T/96T
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