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英文名称 Anti-C1orf77
中文名称 1号染色体开放阅读框77抗体
别 名 Chromatin target of PRMT1 protein; Chromosome 1 open reading frame 77; CHTOP; CHTOP_HUMAN; DKFZp547E1010; FOP; Friend of PRMT1 protein; MGC131924; MGC86949; OTTHUMP00000035122; OTTHUMP00000035123; Pp7704; RP1 178F15.2; Small arginine- and glycine-rich protein; Small protein rich in arginine and glycine; SRAG.
浓 度 1mg/1ml
规 格 0.2ml/200μg
1号染色体开放阅读框77抗体概述:
B淋巴细胞在抗原的刺激下,能够分化、增殖形成具有针对这种抗原分泌特异性抗体的能力。B细胞的这种能力和量是有限的,不可能持续分化增殖下去,因此产生免疫球蛋白的能力也是极其微小的。将这种B细胞与非分泌型的骨髓瘤细胞融合形成杂交瘤细胞,再进一步克隆化,这种克隆化的杂交瘤细胞是既具有瘤细胞的无限分裂的能力,又具有产生特异性抗体的B淋巴细胞的能力。将这种克隆化的杂交瘤细胞进行培养或注入小鼠腹水内即可获得大量的高效、单一的特异性抗体。这种技术即称为单克隆抗体技术。
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep
产品类型 一抗
研究领域 细胞生物 免疫学
蛋白分子量 predicted molecular weight: 26kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human C1orf77
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 C1orf77, also known as Friend of PRMT1 protein, is a 248 amino acid protein that plays an essential role in the ligand-dependent activation of estrogen receptor target genes. C1orf77 is tightly associated with chromatin and is modified by both asymmetric and symmetric arginine methylation. Depletion of C1orf77 results in almost complete block of estradiol-induced promter occupancy by the estrogen receptor. Also, complete knockdown of C1orf77 mRNA in adult erythroid progenitors stongly induces fetal hemoglobin, suggesting that C1orf77 is a critical modulator of γ-globin gene expression. There are two isoforms of C1orf77 that are produced as a result of alternative splicing events. The gene encoding C1orf77 maps to human chromosome 1, the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. There are about 3,000 genes on chromosome 1, and considering the great number of genes there are also a large number of diseases associated with chromosome 1.
Function : Plays an important role in the ligand-dependent activation of estrogen receptor target genes. May play a role in the silencing of fetal globin genes. Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.
Subunit : Interacts with PRMT1 and PRMT5. Interacts with the 5FMC complex; the interaction is methylation-dependent. Interacts with FYTTD1, SET and PRC1 complex members CBX4, RNF2 and PHC2; the interactions are methylation-independent. Interacts with ZNF148 (By similarity).
Subcellular Location : Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm (By similarity). Note=Mostly associated with facultative heterochromatin (By similarity).
Tissue Specificity : Expressed in an erythroid progenitor cell line derived from peripheral blood.
1号染色体开放阅读框77抗体Post-translational modifications : Asymmetrically methylated by PRMT1. Symmetrically methylated by PRMT5 (By similarity).
Database links : UniProtKB/Swiss-Prot: Q9Y3Y2.2