磷酸化视网膜母细胞瘤样蛋白p107抗体

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英文名称  Anti-phospho-p107/RBL1(Ser975)

中文名称  磷酸化视网膜母细胞瘤样蛋白p107抗体

别    名  RBL1(phospho S975); Cellular protein 107; CP107; PRB1; RBL1; Retinoblastoma like protein 1; RBL1_HUMAN.

浓    度  1mg/1ml

规 格  0.1ml/100μg

磷酸化视网膜母细胞瘤样蛋白p107抗体概述：

B淋巴细胞在抗原的刺激下，能够分化、增殖形成具有针对这种抗原分泌特异性抗体的能力。B细胞的这种能力和量是有限的，不可能持续分化增殖下去，因此产生免疫球蛋白的能力也是极其微小的。将这种B细胞与非分泌型的骨髓瘤细胞融合形成杂交瘤细胞，再进一步克隆化，这种克隆化的杂交瘤细胞是既具有瘤细胞的无限分裂的能力，又具有产生特异性抗体的B淋巴细胞的能力。将这种克隆化的杂交瘤细胞进行培养或注入小鼠腹水内即可获得大量的高效、单一的特异性抗体。这种技术即称为单克隆抗体技术。

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep

产品类型  一抗  磷酸化抗体  

研究领域  肿瘤 免疫学 信号转导 干细胞 细胞周期蛋白 转录调节因子  

蛋白分子量  predicted molecular weight: 121kDa

性    状  Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human RBL1 around the phosphorylation site of Ser975

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 The pocket protein family consists of three structurally and functionally related proteins, Rb (retinoblastoma), p107, and p130. This family of tumor suppressors function to regulate important cellular transcription factors, such as the E2F family. The E2F proteins regulate the expression of genes whose products are important for cell cycle progression. The inactivation Rb is catalyzed by CDK phosphorylation thereby releasing E2F during the G1-S phase cellular progression. Unchecked inactivation of Rb in G1 phase has been indicated as a universal mechanism underlying cellular transformation . While Rb has been the most studied among the pocket proteins, p107 and p130 have also been shown to be key regulators of E2F. Several studies have also provided evidence that p107/p130 provide different functions in E2F regulation than does Rb. Rb, p107, and p130 each contain a conserved 'A/B pocket', which is the target of several viral oncoproteins, namely SV40 large T-antigen and adenovirus E1A. There are two isoforms.

Function : Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.

Subunit : Interacts with SUV420H1, SUV420H2 and USP4 (By similarity). Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with AATF. Interacts with KDM5A. Interacts with SV40 and JC virus large T antigens.

Subcellular Location : Nucleus.

磷酸化视网膜母细胞瘤样蛋白p107抗体Post-translational modifications : Exists in both phosphorylated and unphosphorylated forms, and T, but not E1A, binds only to the unphosphorylated form. Cell-cycle arrest properties are inactivated by phosphorylation on Thr-332, Ser-640, Ser-964 and Ser-975 by CDK4.

Similarity : Belongs to the retinoblastoma protein (RB) family.

Database links : UniProtKB/Swiss-Prot: P28749.3