本公司是最专业增殖潜能相关蛋白抗体供应商,提供增殖潜能相关蛋白抗体的报价,RhoC抗体咨询,技术服务,欢迎来电咨询选购。
英文名称 Anti-RBBP6/P2P-R
中文名称 增殖潜能相关蛋白抗体
别 名 Retinoblastoma-binding protein 6; proliferation potential protein related; p53-associated cellular protein of testis; Proliferation potential-related protein; Retinoblastoma-binding Q protein 1; Protein RBQ-1; My038; PACT; Protein P2P-R; RBBP6_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
增殖潜能相关蛋白抗体概述:
B淋巴细胞在抗原的刺激下,能够分化、增殖形成具有针对这种抗原分泌特异性抗体的能力。B细胞的这种能力和量是有限的,不可能持续分化增殖下去,因此产生免疫球蛋白的能力也是极其微小的。将这种B细胞与非分泌型的骨髓瘤细胞融合形成杂交瘤细胞,再进一步克隆化,这种克隆化的杂交瘤细胞是既具有瘤细胞的无限分裂的能力,又具有产生特异性抗体的B淋巴细胞的能力。将这种克隆化的杂交瘤细胞进行培养或注入小鼠腹水内即可获得大量的高效、单一的特异性抗体。这种技术即称为单克隆抗体技术。
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat
产品类型 一抗
研究领域 肿瘤 细胞生物 发育生物学 信号转导 细胞周期蛋白
蛋白分子量 predicted molecular weight: 201kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from humna P2PR N-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 P2P-R/RBBP6 (Retinoblastoma- binding protein 6; proliferation potential protein related; p53-associated cellular protein of testis; Proliferation potential-related protein; Retinoblastoma-binding Q protein 1; Protein RBQ-1; My038; PACT; Protein P2P-R;) interacts with p53/TP53 and RB1 (By similarity). [SUBCELLULAR LOCATION] Nucleus, nucleolus. [TISSUE SPECIFICITY] Highly expressed in the placenta and testis. Expressed at lower levels in the brain, heart, kidney, liver and lung. Overexpressed in esophageal cancer.
Function : E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth.
Subunit : Interacts with p53/TP53 and RB1 (By similarity). Interacts also with MDM2 and YBX1. Interacts with NEK6.
Subcellular Location : Nucleus, nucleolus. Chromosome. Cytoplasm, cytoskeleton, centrosome. Note=Colocalizes with mitotic chromosomes. Co-localizes with NEK6 in the centrosome.
Tissue Specificity : Highly expressed in the placenta and testis. Expressed at lower levels in the brain, heart, kidney, liver and lung. Overexpressed in esophageal cancer.
Post-translational modifications : Phosphorylated by NEK6.
Similarity : Contains 1 CCHC-type zinc finger.
Contains 1 DWNN domain.
Contains 1 RING-type zinc finger.
增殖潜能相关蛋白抗体Database links : UniProtKB/Swiss-Prot: Q7Z6E9.1
信号传导(Signaling Intermediates)
P2P-P又称PACT即P53相关细胞蛋白(P53 associated cellular protein-testes derived), P2P-P蛋白是p53的负调控因子,而且在细胞生长和胚胎发育,细胞分化、前体mRNA剪接、周期阻滞、凋亡过程中起到了重要作用。