本公司是最专业环指蛋白111抗体供应商,提供环指蛋白111抗体的报价,泛素样蛋白Sumo2/3抗体咨询,
英文名称 Anti-RNF111
中文名称 环指蛋白111抗体
别 名 ARK; Arkadia; DKFZp313E0731; DKFZp686H1966; DKFZp761D081; E3 ubiquitin protein ligase Arkadia; FLJ38008; Ring finger protein 111; RNF 111; RN111_HUMAN.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
环指蛋白111抗体概述:
B淋巴细胞在抗原的刺激下,能够分化、增殖形成具有针对这种抗原分泌特异性抗体的能力。B细胞的这种能力和量是有限的,不可能持续分化增殖下去,因此产生免疫球蛋白的能力也是极其微小的。将这种B细胞与非分泌型的骨髓瘤细胞融合形成杂交瘤细胞,再进一步克隆化,这种克隆化的杂交瘤细胞是既具有瘤细胞的无限分裂的能力,又具有产生特异性抗体的B淋巴细胞的能力。将这种克隆化的杂交瘤细胞进行培养或注入小鼠腹水内即可获得大量的高效、单一的特异性抗体。这种技术即称为单克隆抗体技术。
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Rabbit, Sheep
产品类型 一抗
研究领域 细胞生物 免疫学
蛋白分子量 predicted molecular weight: 109kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human RNF111
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 RNF111 (Ring finger protein 111) acts in the NODAL pathway of mesoderm patterning during embryonic development. It is an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. It also induces degradation of the transriptional repressors SnoN and c-Ski. RNF111, therefore, effectively enhances TGF-beta signaling through simultaneous down-regulation of two distinct types of negative regulators, Smad7 and SnoN/c-Ski.
Function : Acts in the NODAL pathway of mesoderm patterning during embryonic development. Acts downstream AXIN1 as an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. Activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation.
Subunit : Interacts with SMAD6, SMAD7, AXIN1, AXIN2 and SKIL isoform SNON. Part of a complex containing RNF111, AXIN1 and SMAD7. Interacts (via SIM domains) with SUMO1 and SUMO2.
Subcellular Location : Cytoplasmic and Nuclear
环指蛋白111抗体Tissue Specificity : Broadly expressed.
Similarity : Contains 1 RING-type zinc finger.
Database links : UniProtKB/Swiss-Prot: Q6ZNA4.3