RAP1GAP酶激活蛋白抗体

本公司是最专业RAP1GAP酶激活蛋白抗体供应商，提供RAP1GAP酶激活蛋白抗体的报价，泛素样蛋白Sumo2/3抗体咨询，

英文名称  Anti-RAP1GAP

中文名称  RAP1GAP酶激活蛋白抗体

别    名  Rap1 GTPase activating protein 1; RAP1 GTPase activating protein; RAP1GA1; Rap1ga1 protein; RAP1GAP1; RAP1GAPII; RAPGAP; KIAA0474; RPGP1_HUMAN.

浓    度  1mg/1ml

规 格  0.2ml/200μg

RAP1GAP酶激活蛋白抗体概述：

B淋巴细胞在抗原的刺激下，能够分化、增殖形成具有针对这种抗原分泌特异性抗体的能力。B细胞的这种能力和量是有限的，不可能持续分化增殖下去，因此产生免疫球蛋白的能力也是极其微小的。将这种B细胞与非分泌型的骨髓瘤细胞融合形成杂交瘤细胞，再进一步克隆化，这种克隆化的杂交瘤细胞是既具有瘤细胞的无限分裂的能力，又具有产生特异性抗体的B淋巴细胞的能力。将这种克隆化的杂交瘤细胞进行培养或注入小鼠腹水内即可获得大量的高效、单一的特异性抗体。这种技术即称为单克隆抗体技术。

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human, Mouse, Rat, Cow, Rabbit  

产品类型  一抗    

研究领域  肿瘤 细胞生物 免疫学 信号转导 激酶和磷酸酶  

蛋白分子量  predicted molecular weight: 73kDa

性    状  Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human RAP1GAP.

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 RAP1GAP, an 85/95kDa protein, is a member of a family of GAPs specific for Rap1. Though to be an important tumor suppressor, Rap1GAP has no sequence homology with other GAPs such as RasGAP, RanGAP and RhoGAP. Purified as a cytosolic and membrane-bound protein, both isoforms consist of two monomers each contain a dimerization and a catalytic domain. Mutations in the GAP domain of the tumor suppressor tuberin, can cause benign tumor phenotype tuberous sclerosis. There are 2 isoforms produced by alternative splicing.

Function : GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.

Subunit : Homodimer and heterodimer with RAP1B.

Subcellular Location : Golgi apparatus membrane; Peripheral membrane protein.

Tissue Specificity : Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.

Similarity : Contains 1 GoLoco domain.

Contains 1 Rap-GAP domain.

Database links : UniProtKB/Swiss-Prot: P47736.2

RAP1GAP酶激活蛋白抗体RaplGAP在细胞的粘附连接及形成过程中所发挥着一定作用。RaplGAP是小分子G蛋白Rapl的GTP酶激活蛋白(GTPase activating protein)，它可以加速rap1由活化形式(与GTP结合)转变为去活化形式(与GDP结合)。由于粘附连接的破坏或延迟了粘附连接形成的过程，对肿瘤转移有着密切的联系。