磷酸化Ras特异性鸟嘌呤核苷酸释放因子1

本公司是最专业磷酸化Ras特异性鸟嘌呤核苷酸释放因子1供应商，提供磷酸化Ras特异性鸟嘌呤核苷酸释放因子1的报价，泛素样蛋白Sumo2/3抗体咨询，

英文名称  Anti-Phospho-RASGRF1(Ser929)

中文名称  磷酸化Ras特异性鸟嘌呤核苷酸释放因子1

别    名  RASGRF1(phospho S929)CDC25; CDC25L; GNRP; GRF1; GRF55; Guanine nucleotide exchange factor; Guanine nucleotide releasing factor 55 kD; Guanine nucleotide releasing protein; HGRF55; PP13187; Ras protein specific guanine nucleotide releasing factor 1; Ras specific guanine nucleotide releasing factor; Ras specific guanine nucleotide releasing factor CDC25 homolog; Ras specific nucleotide exchange factor CDC25.

浓    度  1mg/1ml

规 格  0.1ml/100μg

磷酸化Ras特异性鸟嘌呤核苷酸释放因子1概述：

B淋巴细胞在抗原的刺激下，能够分化、增殖形成具有针对这种抗原分泌特异性抗体的能力。B细胞的这种能力和量是有限的，不可能持续分化增殖下去，因此产生免疫球蛋白的能力也是极其微小的。将这种B细胞与非分泌型的骨髓瘤细胞融合形成杂交瘤细胞，再进一步克隆化，这种克隆化的杂交瘤细胞是既具有瘤细胞的无限分裂的能力，又具有产生特异性抗体的B淋巴细胞的能力。将这种克隆化的杂交瘤细胞进行培养或注入小鼠腹水内即可获得大量的高效、单一的特异性抗体。这种技术即称为单克隆抗体技术。

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep

产品类型  一抗  磷酸化抗体  

研究领域  肿瘤 细胞生物 免疫学 信号转导 转录调节因子  

蛋白分子量  predicted molecular weight: 145kDa

性    状  Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human RasGRF1 around the phosphorylation site of Ser929

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 RASGRF1 stimulates the dissociation of GDP from RAS protein. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Function : Promotes the exchange of Ras-bound GDP by GTP.

Subunit : Homooligomer and heterooligomer with RASGRF2. Interacts with USP8, thereby regulating its stability (By similarity).

Post-translational modifications : Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome (By similarity).

Ubiquitinated and degraded following phosphorylation by PLK2 By similarity).

Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity.

Similarity : Contains 1 DH (DBL-homology) domain.

Contains 1 IQ domain.

磷酸化Ras特异性鸟嘌呤核苷酸释放因子1Contains 1 N-terminal Ras-GEF domain.

Contains 2 PH domains.

Contains 1 Ras-GEF domain.

Database links : UniProtKB/Swiss-Prot: Q13972.1