CyTOF?质谱流式细胞术——The Scientist杂志评选出的2011年十大创新技术。
CyTOF质谱流式细胞术,采用金属元素标记物(通常是金属元素标记的特异抗体或者染料)标记或识别细胞表面和内部的信号分子,然后用流式细胞原理分离单个细胞,再用感应耦合等离子质谱(ICP-MS)观察单个细胞的原子质量谱,最后将原子质量谱的数据转换为细胞表面和内部的信号分子数据,并通过专业分析软件对获得的数据进行分析,从而实现对细胞表型和信号网络的精细观察。
这项研究通过这个可以称为大量细胞计数法的方法,观察了人类骨髓产生的不同形态细胞中及表面的34种物质,不但能正确归类10多种不同类型的免疫细胞,还能观察到各类免疫细胞的内部变化,从而预知可能发生的变化。这将有助于更快更广泛的测量处方药对人体细胞的反应及功效,提前发现细胞病变,研发出针对个人的治疗药物。
多数商业和学术用途中已报道适用于CyTOF?技术的抗体,都可以在eBioscience找到:
Name | Clone | Application | Cat. No. |
Anti-Human CD3 Functional Grade Purified | UCHT1 | FC, FA | 16-0038 |
Anti-Human CD3 Purified | UCHT1 | FC, IHC, FA | 14-0038 |
Anti-Mouse CD3e Functional Grade Purified | 145-2C11 | FC, FA | 16-0031 |
Anti-Mouse CD3e Purified | 145-2C11 | FC, IHC, FA, IP, WB | 14-0031 |
Anti-Mouse CD4 Functional Grade Purified | RM4-5 | FC | 16-0042 |
Anti-Mouse CD4 Purified | RM4-5 | FC, IHC, FA | 14-0042 |
Anti-Human CD4 Functional Grade Purified | RPA-T4 | FC, FA | 16-0049 |
Anti-Human CD4 Purified | RPA-T4 | FC, IHC, FA | 14-0049 |
Anti-Mouse CD5 Purified | 53-7.3 | FC, IHC, IP | 14-0051 |
Anti-Mouse CD8a Functional Grade Purified | 53-6.7 | FC, FA | 16-0081 |
Anti-Mouse CD8a Purified | 53-6.7 | FC, IHC, IP | 14-0081 |
Anti-Human CD8a Purified | RPA-T8 | FC, IHC | 14-0088 |
Anti-Human CD9 Purified | eBioSN4 (SN4 C3-3A2) | FC, IHC, IP | 14-0098 |
Anti-Human CD11b Purified | ICRF44 | FC, IHC, FA | 14-0118 |
Anti-Mouse CD11b Functional Grade Purified | M1/70 | FC, FA | 16-0112 |
Anti-Mouse CD11b Purified | M1/70 | FC, IHC, FA, IP | 14-0112 |
Anti-Human CD11c Purified | 3.9 | FC, IHC | 14-0116 |
Anti-Mouse CD11c Functional Grade Purified | N418 | FC | 16-0114 |
Anti-Mouse CD11c Purified | N418 | FC, IHC, IP | 14-0114 |
Anti-Human CD13 Purified | WM-15 (WM15) | FC, IHC | 14-0138 |
Anti-Human CD19 Functional Grade Purified | HIB19 | FC, FA | 16-0199 |
Anti-Human CD19 Purified | HIB19 | FC, IHC, FA | 14-0199 |
Anti-Human CD20 Purified | 2H7 | FC, IHC | 14-0209 |
Anti-Human CD27 Purified | O323 | FC | 14-0279 |
Anti-Human CD28 Functional Grade Purified | CD28.2 | FC, FA | 16-0289 |
更多产品请见链接: http://us.ebioscience.com/application/flow-cytometry/cytof.htm?p=3
参考文献:
Bendall, S.C., et al., Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum.
Science. 332(6030): p. 687-96.
http://www.ncbi.nlm.nih.gov/pubmed/21551058/
阅读原文:http://www.liankebio.com/ProductCenterShow/articleID/2014040016.html
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