SANTA CRUZ BIOTECHNOLOGY, INC.
EGF抗体(F-9): sc-166779
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com
BACKGROUND
Epidermal growth factor (EGF) is an acid- and heat-stable 53 amino acid
protein originally found in rodents and humans. It has been shown to be a
potent mitogen for a variety of cell types both in vivo and in vitro. EGF binds
to the EGF receptor on the surface of cells and mediates intrinsic phosphorylation
of the receptor on tyrosine residues. It has been detected in nearly all
body fluids, such as urine (urogastrone), saliva, milk and platelet-rich plasma.
EGF, TGFα and vaccinia virus growth factor exhibit 30-40% amino acid
homology. Several additional members of the EGF/TGF family have been
described; these include Cripto, Amphiregulin and the heparin-binding EGFlike
growth factor. Amphiregulin and the heparin-binding EGF-like growth
factor both bind to the EGF receptor.
REFERENCES
1. Cohen, S. 1962. Isolation of a mouse submaxillary gland protein accelerating
incisor eruption and eyelid opening in the newborn animal. J. Biol.
Chem. 237: 1555-1562.
2. Gregory, H. 1985. In vivo aspects of urogastrone-epidermal growth factor.
J. Cell Sci. Suppl. 3: 11-17.
3. Stroobant, P., et al. 1985. Purification and characterization of vaccinia virus
growth factor. Cell 42: 383-393.
4. Carpenter, G., et al. 1986. Epidermal growth factor, its receptor, and related
proteins. Exp. Cell Res. 164: 1-10.
5. Derynck, R. 1986. Transforming growth factor α: structure and biological
activities. J. Cell. Biochem. 32: 293-304.
6. Carpenter, G. 1987. Receptors for epidermal growth factor and other polypeptide
mitogens. Annu. Rev. Biochem. 56: 881-914.
7. Starkey, R.H., et al. 1987. Radioimmunoassay of human epidermal growth
factor (urogastrone). J. Clin. Endocrinol. Metab. 45: 1144-1153.
8. Ciardiello, F., et al. 1991. Differential expression of epidermal growth factorrelated
proteins in human colorectal tumors. Proc. Natl. Acad. Sci. USA 88:
7792-7796.
CHROMOSOMAL LOCATION
Genetic locus: EGF (human) mapping to 4q25.
SOURCE
EGF (F-9) is a mouse monoclonal antibody raised against amino acids
971-1023 representing mature EGF of human origin.
PRODUCT
Each vial contains 200 μg IgG1 in 1.0 ml of PBS with < 0.1% sodium azide
and 0.1% gelatin.
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
APPLICATIONS
EGF (F-9) is recommended for detection of precursor and mature EGF of
human origin by Western Blotting (starting dilution 1:100, dilution range
1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein
(1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution
range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution
range 1:30-1:3000).
Suitable for use as control antibody for EGF siRNA (h): sc-39416, EGF shRNA
Plasmid (h): sc-39416-SH and EGF shRNA (h) Lentiviral Particles: sc-39416-V.
Molecular Weight of EGF precursor: 160 kDa.
Molecular Weight of mature EGF: 6 kDa.
Positive Controls: human platelet extract: sc-363773.
RECOMMENDED SECONDARY REAGENTS
To ensure optimal results, the following support (secondary) reagents are
recommended: 1) Western Blotting: use goat anti-mouse IgG-HRP: sc-2005
(dilution range: 1:2000-1:32,000) or Cruz Marker? compatible goat antimouse
IgG-HRP: sc-2031 (dilution range: 1:2000-1:5000), Cruz Marker?
Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:
sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecipitation:
use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml).
3) Immunofluorescence: use goat anti-mouse IgG-FITC: sc-2010 (dilution
range: 1:100-1:400) or goat anti-mouse IgG-TR: sc-2781 (dilution range:
1:100-1:400) with UltraCruz? Mounting Medium: sc-24941.
DATA
RESEARCH USE
For research use only, not for use in diagnostic procedures.
PROTOCOLS
See our web site at www.scbt.com or our catalog for detailed protocols and
support products.
EGF (F-9): sc-166779. Western blot analysis of EGF
expression in human platelet extract.
132 K –
90 K –
precursor
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