Anti-NLRP3抗体说明书，进口一抗

Rabbit Anti-NLRP3进口一抗 antibody

Catalog Number :RS-81021

Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ） Background: background:
May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.

DISEASE:

Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.

Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.

Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation.

Specificity :

–Anti-NLRP3 is a rabbit polyclonal antibody

–specific for NLRP3 of chiken,rat ,mouse ,hu

– use for western blotting,elisa,immunoprecipitation and immunohistochemistry

–Protein G affinity chromatography purification, purity :>95%

– Isotype: IgG

– mol wt: 114kDa

Application :

WB=1:100-500

ELISA=1:500-1000

IHC-P=1:100-500

IHC-F=1:100-500

IF=1:100-500

– KLH conjugated synthetic peptide derived from human IL-8 C-terminus

– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.