一次实验完成96通道高通量全自动分析。无需跑胶,无需转膜,无需人工干预。自动加样,自动分离,自动孵育,完成全部Western blot 过程,并实现绝对产量和相对定量分析。是Simple Western专利技术的新一代产品。
【简单分析】
Sally Sue will run the entire Simple Western assay for you. Simply load your samples, push a button and walk away. Sally Sue
will separate proteins by size, detect your target proteins and even quantitate them using a standard curve.
![](http://www.ndchina.com.cn/common-uploads/2014/12/up_14188023619163996_0.jpg)
Figure 1. Detection of PI3 Kinase in a serial dilution of HeLa lysate (1000 ng/μL – 37 ng/μL). All eight cycles showed similar results (CV <15%). Linearity of the dilution series (R2=0.989) is maintained even at very
low protein concentrations.
Sometimes you wish you could do more with less. Sally Sue does up to 96 samples in one experiment with as little as 0.2 μg/μL protein in the sample. She also sends a tweet when results are ready just in case you lose track of your new hands-free time.
![](http://www.ndchina.com.cn/common-uploads/2014/12/up_14188024024791282_0.jpg)
Figure 2. Sally Sue can analyze an entire cell signaling pathway in a single run. HeLa cells were left untreated or treated with
TNFα to stimulate the NFκB signaling pathway. Using only 5 μL/well (1 μgμL) of either a whole cell lysate (WC) or nuclear
extract (NE) from those cells, Sally Sue was able to quantitate seven different proteins from the pathway plus a loading
control (αTubulin). She even threw in an extra replicate for good measure.
关于PS公司
美国ProteinSimple公司 (简称 PS公司)是一家致力于定义未来蛋白质分析的生命科学公司,它结合了Alpha Innotech、Cell Biosciences、Protein Forest、Brightwell Technologies和Convergent Bioscience等行业领先品牌,为生命科学、医学、制药和生物公司提供简单、精确和高效的技术平台。
总部位于加州美国硅谷,在美国加州、加拿大多伦多、加拿大渥太华等地设有生产和研发部门,同时在全球多地设有办事处。PS公司重视中国市场的发展,在北京、上海,广州和武汉设有办事处和技术服务中心,为用户提供完善的技术支持和售后服务。