磷酸化Disabled 1抗体，Anti-Phospho-Dab1 (Ser491)

磷酸化Disabled 1抗体英文名称  Anti-Phospho-Dab1 (Ser491) 

中文名称  磷酸化Disabled 1抗体 

别    名  Dab 1; Disabled homolog 1; Disabled homolog 1 Drosophila; Scm; Scr; Scrambler; Yot; Yotari. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg          

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep  

产品类型  一抗  磷酸化抗体   

研究领域  神经生物学  

蛋白分子量  predicted molecular weight: 65kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human Dab1 around the phosphorylation site of Ser491 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

磷酸化Disabled 1抗体产品介绍 Disabled 1 (Dab1) is an 80 kDa protein that is encoded by the Disabled-1 gene locus which is mutated in scrambler and yotari mutant mice. Phenotypically, the mutation of this gene produces motor defects and ataxia, disruption of neuronal migration, and severe cerebellar hypoplasia. Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by the secreted protein reelin. Dab1 contains a phosphotyrosine binding (PTB) domain in the amino terminus. Tyrosine phosphorylation of Dab1 is increased by reelin binding to the Very Low Density Lipoprotein Receptor (VLDLR) and Apolipoprotein E Receptor 2 (ApoER2) through stimulation of Src family kinases. Src family kinase and c-Abl activities are themselves then stimulated by binding to tyrosine phosphorylated Dab1. Dab1 also mediates activation of Akt (PKB) by reelin resulting in inhibition of glycogen synthase kinase 3 beta (GSK-3 beta) and decreased phosphorylation of the microtubule-associated protein, Tau. Dab1 serine 491 is phosphorylated in a Cdk5-dependent manner and regulates, likely indirectly, Reelin-induced signaling during neural cortex development.

Function : Adapter molecule functioning in neural development. May regulate SIAH1 activity.

Subunit : Associates with the SH2 domains of SRC, FYN and ABL. Interacts with DAB2IP and SIAH1. Interacts with LRP1.

Post-translational modifications : Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons. Also phosphorylated on Ser-524 independently of reelin signaling.

Similarity : Contains 1 PID domain.