胞浆接头蛋白Dok5抗体

胞浆接头蛋白Dok5抗体英文名称  Anti-DOK5/IRS6 

中文名称  胞浆接头蛋白Dok5抗体 

别    名  chromosome 20 open reading frame 180; Docking protein 5; DOK 5; DOK5 protein; downstream of tyrosine kinase 5; IRS6. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg          

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit   

产品类型  一抗    

研究领域  细胞生物 信号转导 激酶和磷酸酶 通道蛋白  

蛋白分子量  predicted molecular weight: 34kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human DOK5/IRS6 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

胞浆接头蛋白Dok5抗体产品介绍 The downstream of kinase family (Dok-1-7) are members of a class of “docking” proteins that include the tyrosine kinase substrates IRS-1 and Cas, which contain multiple tyrosine residues and putative SH2 binding sites. Dok-4 and Dok-5 are more similar to each other than to the other Dok family members, and may constitute a subfamily of the DOK genes. Dok-5 is a tyrosine kinase substrate that enhances c-Ret-dependent activation of mitogen-activated protein kinase (MAPK). Dok-5 transcript is abundant in muscle and increases during T cell activation. Dok-5 protein undergoes tyrosine phosphorylation in response to Insulin and Insulin-like growth factor-1. The gene encoding human Dok-5 maps to chromosomal location 20q13.2. 

Function : DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation.

Subunit : Interacts with phosphorylated RET. In contrast to other DOK proteins, it does not interact with RASGAP (By similarity).

Tissue Specificity : Highest expression in skeletal muscle, lower in brain, heart and kidney. Also detected in activated peripheral blood T-lymphocytes.

Post-translational modifications : Phosphorylated on tyrosine residues in response to insulin, IGF1 and GDNF.

Similarity : Belongs to the DOK family. Type B subfamily. 

Contains 1 IRS-type PTB domain. 

Contains 1 PH domain.