有丝分裂控制样蛋白DIS3L抗体

有丝分裂控制样蛋白DIS3L抗体英文名称  Anti-DIS3L 

中文名称  有丝分裂控制样蛋白DIS3L抗体 

别    名  DIS3 like exonuclease 1; DIS3 mitotic control homolog (S. cerevisiae) like; FLJ38088; KIAA1955; MGC4562; DI3L1_HUMAN. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg          

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Pig, Horse   

产品类型  一抗    

研究领域  细胞生物 免疫学 表观遗传学  

蛋白分子量  predicted molecular weight: 121kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human DIS3L 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

有丝分裂控制样蛋白DIS3L抗体保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The exosome is a multi-protein complex composed of several highly conserved subunits, some of which are 3’ to 5’ exoribonucleases. The complex is involved in a variety of cellular processes and is responsible for degrading unstable mRNAs that contain AU-rich elements (AREs) in their untranslated 3’ regions. DIS3, also known as RRP44, is a 958 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one PINc domain. Widely expressed with highest expression in testis, DIS3 functions as a component of the exosome exoribonuclease complex and is required for processing of 7S pre-RNA into a mature nuclear complex and, ultimately, for proper mitotic progression. Abnormal expression levels of DIS3 may be associated with colon cancer, suggesting a role for DIS3 in tumorigenesis. Multiple isoforms of DIS3 exist due to alternative splicing events.

Function : Putative cytoplasm-specific catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. 

Subunit : Component of the RNA exosome complex. The catalytically inactive RNA exosome core (Exo-9) complex is believed to associate with catalytic subunits EXOSC10, and DIS3 or DIS3L in cytoplasmic-and nuclear-specific RNA exosome complex forms. 

Subcellular Location : Cytoplasm.

Similarity : Belongs to the RNR ribonuclease family. 

Database links :