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DENND1B蛋白抗体

供货周期： 7天

品牌： Abcam

型号： 0.1ml/100μg

货号：

报价： ¥1

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产品介绍

DENND1B蛋白抗体英文名称 Anti-DENND1B

中文名称 DENND1B蛋白抗体

别名 C1ORF18; C1orf218; Chromosome 1 open reading frame 218; Connecdenn 2; DEN1B_HUMAN; DENN domain containing protein 1B; DENN domain-containing protein 1B; DENN MADD domain containing 1B; DENND1B; DKFZp547O0715; FAM31B; Family with sequence similarity 31 member B; FLJ20054; FLJ21179; FLJ33888; MGC103810; MGC27044; Protein FAM31B

公司DENND1B蛋白抗体应用于医学免疫学、动物免疫学、分子生物学、生物化学、临床医学、检验医学、动物医学、药学、理工、农学、环境学等生命科学密切相关的专业。

浓度 1mg/1ml

规格 0.2ml/200μg

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Rabbit

产品类型一抗

研究领域细胞生物免疫学

蛋白分子量 predicted molecular weight: 48kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human DENND1B

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

DENND1B蛋白抗体产品介绍 Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactived RAB35, then activates RAB35 for its subsequent function at endosomes.

Tissue specificity:Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T cells as compared with naive T cells.

Function : Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactivates RAB35, then activates RAB35 for its subsequent function at endosomes.

Subunit : Interacts with RAB35 in the absence of nucleotide, but not in the presence of GTP. Interacts with clathrin heavy chain/CLTC and with AP2A2. Isoform 5 interacts with AP2B1.

Subcellular Location : Cytoplasm, cytosol. Cytoplasmic vesicle, clathrin-coated vesicle.

Tissue Specificity : Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T-cells as compared with naive T-cells.

Post-translational modifications : Isoform 5 is phosphorylated on Tyr-475, Tyr-484, Ser-514 and Ser-652.

Similarity : Contains 1 dDENN domain.

Contains 1 DENN domain.

Contains 1 uDENN domain.

【存储要求】连续使用时4°C存储，保质期六个月；DENND1B蛋白抗体期存储时建议分装为10ul以上小包装-20°C存储，并避免反复冻融，保质期一年。

实验的用途：

1）WB：Western Blotting 免疫印迹

2）IH：Immunohistochemistry 免疫组化

3）IH(P)：Immunohistochemistry Parraffin sections 免疫组化(石蜡)

4）IH(F)：Immunohistochemistry Frozen sections 免疫组化(冰冻)

5）IC：Immunocytochemistry 免疫细胞化学