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磷酸化形态发生紊乱关联激活因子1抗体

供货周期： 7天

品牌： Abcam

型号： 0.1ml/100μg

货号：

报价： ¥1

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产品介绍

磷酸化形态发生紊乱关联激活因子1抗体英文名称 Anti-phospho-DAAM1 (Thr361)

中文名称磷酸化形态发生紊乱关联激活因子1抗体

别名 DAAM 1; DAAM-1; Dishevelled associated activator of morphogenesis 1; FLJ41657; KIAA0666.

公司磷酸化形态发生紊乱关联激活因子1抗体应用于医学免疫学、动物免疫学、分子生物学、生物化学、临床医学、检验医学、动物医学、药学、理工、农学、环境学等生命科学密切相关的专业。

浓度 1mg/1ml

规格 0.1ml/100μg

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Horse, Rabbit, Sheep, Guinea Pig

产品类型一抗磷酸化抗体

研究领域肿瘤免疫学

蛋白分子量 predicted molecular weight: 120-130kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated Synthesised phosphopeptide derived from human DAAM1 around the phosphorylation site of Thr361 [SA(p-T)QM]

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

磷酸化形态发生紊乱关联激活因子1抗体产品介绍 DAAM1 (Dishevelled associated activator of morphogenesis 1) is a diaphanous-related formin which binds to dishevelled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex assembly. It is an essential component of noncanonical Wnt signaling. DAAM1 also communicates with Rho GTPases, CIP4 and Src in the regulation of the signalling pathways that co-ordinate the dynamics of the actin filament system. DAAM1 may be a coordinator of endocytosis and cytoskeletal remodeling.

Function : Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments.

Subunit : Homodimer. Interacts with CIP4, FNBP1 and FNBP1L. Interacts with the SH3 domains of Abl, BTK, endophilin, spectrin and SRC. Binds specifically to GTP-bound CDC42 and RHOA.

Subcellular Location : Cytoplasm. Note=Perinuclear.

Tissue Specificity : Expressed in all tissues examined.

Similarity : Belongs to the formin homology family.

Contains 1 DAD (diaphanous autoregulatory) domain.

Contains 1 FH1 (formin homology 1) domain.

Contains 1 FH2 (formin homology 2) domain.

Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.

Database links : UniProtKB/Swiss-Prot: Q9Y4D1.2

DAAM1是蓬乱蛋白相关形态形成活化因子1或称形态发生紊乱关联激活因子1，为形成素蛋白家族的成员。Daam1是WNT/PCP通路的组成因子之一,参与细胞的内吞功能,可调节细胞骨架的形成与构建.

【存储要求】连续使用时4°C存储，保质期六个月；磷酸化形态发生紊乱关联激活因子1抗体期存储时建议分装为10ul以上小包装-20°C存储，并避免反复冻融，保质期一年。

实验的用途：

1）WB：Western Blotting 免疫印迹

2）IH：Immunohistochemistry 免疫组化

3）IH(P)：Immunohistochemistry Parraffin sections 免疫组化(石蜡)

4）IH(F)：Immunohistochemistry Frozen sections 免疫组化(冰冻)

5）IC：Immunocytochemistry 免疫细胞化学