您好，欢迎访问仪器信息网

搜全站

搜展位

上海基免实业有限公司

关注

已关注

已认证

粉丝量 0

全部分类

首页

DNA聚合酶λ/DNA pol λ抗体

供货周期： 7天

品牌： Abcam

型号： 0.2ml/200μg

货号：

报价： ¥1

留言咨询

产品介绍

DNA聚合酶λ/DNA pol λ抗体英文名称 Anti-DNA Polymerase lambda

中文名称 DNA聚合酶λ/DNA pol λ抗体

别名 BETA N; BETAN; DNA directed DNA polymerase lambda; DNA polymerase beta 2; DNA polymerase beta-2; DNA polymerase kappa; DNA polymerase kappa DNA polymerase beta N; DNA polymerase lambda; DNA polymerase lamda2; DPOLL_HUMAN; EC 2.7.7.7,EC 4.2.99.; FLJ46002; OTTHUMP00000020321; OTTHUMP00000020323; OTTHUMP00000059179; Pol beta2; POL KAPPA; Pol Lambda; POLKAPPA; POLL; Polymerase DNA directed lambda.

公司DNA聚合酶λ/DNA pol λ抗体应用于医学免疫学、动物免疫学、分子生物学、生物化学、临床医学、检验医学、动物医学、药学、理工、农学、环境学等生命科学密切相关的专业。

浓度 1mg/1ml

规格 0.2ml/200μg

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep

产品类型一抗

研究领域细胞生物表观遗传学

蛋白分子量 predicted molecular weight: 63kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human DNA Polymerase lambda

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

DNA聚合酶λ/DNA pol λ抗体产品介绍 DNA polymerase lambda (pol lambda), also designated DNA polymerase lambda or Pol Beta2, is a low-fidelity polymerase which plays a role in both spontaneous and DNA damage-induced mutagenesis. Encoded by the POLL gene, pol lambda is a member of the DNA polymerase type-X family. Pol lambda extends primer-terminal mispairs opposite nondamaged DNA templates, suggesting that it may assist in extending mismatched base pairs during normal DNA replication. In addition, pol ?may play a role in the mutagenic bypass of T-T dimers. Proliferating cell nuclear antigen (PCNA), a protein essential to DNA replication, interacts with pol lambda and thus influences the ability of pol ?to synthesize DNA.

Function : Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.

Subunit : Binds PCNA.

Subcellular Location : Nucleus.

Tissue Specificity : Expressed in a number of tissues. Abundant in testis.

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity : Belongs to the DNA polymerase type-X family.

Contains 1 BRCT domain.

Database links : UniProtKB/Swiss-Prot: Q9UGP5.1

【存储要求】连续使用时4°C存储，保质期六个月；DNA聚合酶λ/DNA pol λ抗体期存储时建议分装为10ul以上小包装-20°C存储，并避免反复冻融，保质期一年。

实验的用途：

1）WB：Western Blotting 免疫印迹

2）IH：Immunohistochemistry 免疫组化

3）IH(P)：Immunohistochemistry Parraffin sections 免疫组化(石蜡)

4）IH(F)：Immunohistochemistry Frozen sections 免疫组化(冰冻)

5）IC：Immunocytochemistry 免疫细胞化学