磷酸化转录因子MYB相关蛋白B抗体,Anti-phospho-B MyB (Thr487)

供货周期： 7天

品牌： Abcam

型号： 0.1ml/100μg

货号：

报价： ¥1

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产品介绍

磷酸化转录因子MYB相关蛋白B抗体英文名称 Anti-phospho-B MyB (Thr487)

中文名称磷酸化转录因子MYB相关蛋白B抗体

别名 B MyB (phospho T487); B MyB (phospho T487);B-Myb; BMyB; MYB L2; Myb related protein B; Myb-like protein 2; Myb-related protein B; MybB; MYBB_HUMAN; MYBL 2; Mybl2; v myb avian myeloblastosis viral oncogene homolog like 2; v myb myeloblastosis viral oncogene homolog (avian) like 2; v myb myeloblastosis viral oncogene homolog like 2.

浓度 1mg/1ml

规格 0.1ml/100μg

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抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat

产品类型一抗磷酸化抗体

研究领域细胞生物信号转导细胞周期蛋白转录调节因子

蛋白分子量 predicted molecular weight: 79kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthesised phosphopeptide derived from human B MyB around the phosphorylation site of Thr487

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

磷酸化转录因子MYB相关蛋白B抗体产品介绍 Myb-Related Protein B (MYBL2), a member of the MYB family of transcription factor genes, is a nuclear protein involved in the regulation of cell survival, proliferation, and differentiation. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes.

Function : Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.

Subunit : Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL22.

Subcellular Location : Nucleus.

Post-translational modifications : Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.

Similarity : Contains 3 HTH myb-type DNA-binding domains.

在纯化抗体时需要控制好几个指标，包括纯度、含量及抗体的抗原结合活性。

纯度：在实验的任何阶段，确定抗体溶液纯度的最简单方法是取一部分样本进行SDS-PAGE电泳。凝胶可用考马斯亮蓝染色(灵敏度为0.1—0.5ug/带)或银染(灵敏度1～l0ug/带)。

定量：如果抗体还不纯，有一个快捷的定量方法，即通过SDS-PAGE电泳分离出轻、重链，然后和已知的标准染色带比较。如果需要分析许多样本，用免疫测定法对抗体定量较容易。如果抗体是经过纯化的，可通过测蛋白总量代替上述两种方法，有一简单的方法，即紫外吸收法。磷酸化转录因子MYB相关蛋白B抗体的量可通过测280nm处的吸收值来测(10D大致相当于0.75mg/m1的纯化抗体)。

抗原结合活性：一般说来，纯化方法不会引起抗原结合活性的改变。用蛋白G或蛋白A树脂很少导致抗体活性丧失。然而，如果最终抗体产物的作用不如原来所预料的好，检测抗体纯化过程所丢失的活性就极为重要。用一系列滴定法比较纯化的抗体和其原材料的活性，以标定每一步中的总抗体量，这将有助于较好的估计通过纯化所丢失的活性。