嘌呤嘧啶核酸内切酶2抗体

嘌呤嘧啶核酸内切酶2抗体英文名称  Anti-APEX2 

中文名称  嘌呤嘧啶核酸内切酶2抗体 

别    名  AP endonuclease 2; AP endonuclease XTH2; APE 2; APE2; APEX 2; APEX L2; APEX nuclease (apurinic/apyrimidinic endonuclease) 2; APEX Nuclease 2; APEX nuclease like 2; APEXL 2; APEXL2; Apurinic apyrimidinic endonuclease 2; Apurinic/apyrimidinic endonuclease 2; Apurinic/apyrimidinic endonuclease like 2; C430040P13Rik; DNA (apurinic or apyrimidinic site) lyase 2; XTH 2; XTH2; APEX2_HUMAN. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg  0.2ml/200μg    

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Dog, Cow   

产品类型  一抗    

研究领域  染色质和核信号  

蛋白分子量  predicted molecular weight: 57kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human APEX2 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

嘌呤嘧啶核酸内切酶2抗体产品介绍 Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair (BER).

Function : Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. 

Subunit : Interacts with PCNA; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA.

Subcellular Location : Nucleus. Cytoplasm. Mitochondrion (Probable). Note=Together with PCNA, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. 

Tissue Specificity : Highly expressed in brain and kidney. Weakly expressed in the fetal brain.

Similarity : Belongs to the DNA repair enzymes AP/ExoA family. 

Database links : UniProtKB/Swiss-Prot: Q9UBZ4.1