抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human
产品类型 一抗
研究领域 肿瘤 细胞生物 免疫学 信号转导 激酶和磷酸酶 细胞骨架 细胞外基质 泛素
蛋白分子量 predicted molecular weight: 52kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human ADAM20
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
去整合素样金属蛋白酶20抗体产品介绍 ADAM20 was first cloned from a human testis library as a protein with homology to the fertilins (ADAMs 1, 2). ADAM20 message was seen predominantly in testis, but also at lower levels in placenta and B-cells. ADAM20 is closely related to ADAM21 (61% identical), ADAM29 (52% identical), and the testases (49-46% identical). ADAM20 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and the initial partial sequence of ADAM21 contained a non-functional catalytic site, making it tempting to speculate that ADAM20 and ADAM21 are orthologs to ADAMs 1&2. Later, a full-length ADAM21 sequence demonstrated a functional catalytic domain, but it may still be that the catalytically dead form of ADAM21 is expressed and functions as a fettilin. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs) the function of ADAM20 is still poorly understood. Other ADAMs family members (ADAM10, ADAM17) have been more thoroughly studied, and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. The full length ADAM20 sequence codes for a 726 amino acid protein, with a predicted mass is 81.711 kD. The sequence containing a Type-I transmembrane domain, metalloproteinase domain, cystein-rich domain, and a short cytoplasmic domain. Unlike many of the other ADAMs proteases, ADAM20 does not appear to contain a furin cleavage site.
Function : May be involved in sperm maturation and/or fertilization.
Subcellular Location : Membrane; Single-pass type I membrane protein.
Tissue Specificity : Testis specific.
Post-translational modifications : Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.
Similarity : Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
Database links : UniProtKB/Swiss-Prot: O43506.2
纯度：在实验的任何阶段，确定抗体溶液纯度的最简单方法是取一部分样本进行SDS-PAGE电泳。凝胶可用考马斯亮蓝染色(灵敏度为0.1—0.5ug/带)或银染(灵敏度1～l0ug/带)。
定量：如果抗体还不纯，有一个快捷的定量方法，即通过SDS-PAGE电泳分离出轻、重链，然后和已知的标准染色带比较。如果需要分析许多样本，用免疫测定法对抗体定量较容易。如果抗体是经过纯化的，可通过测蛋白总量代替上述两种方法，有一简单的方法，即紫外吸收法。去整合素样金属蛋白酶20抗体的量可通过测280nm处的吸收值来测(10D大致相当于0.75mg/m1的纯化抗体)。
抗原结合活性：一般说来，纯化方法不会引起抗原结合活性的改变。用蛋白G或蛋白A树脂很少导致抗体活性丧失。然而，如果最终抗体产物的作用不如原来所预料的好，检测抗体纯化过程所丢失的活性就极为重要。用一系列滴定法比较纯化的抗体和其原材料的活性，以标定每一步中的总抗体量，这将有助于较好的估计通过纯化所丢失的活性。
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