重组膜粘连蛋白5抗体

重组膜粘连蛋白5抗体英文名称  Anti-Annexin V 

中文名称  重组膜粘连蛋白5抗体 

别    名  Anchorin CI; Annexin 5; Annexin A5; Annexin V; Annexin5; AnnexinA5; AnnexinV; ANX 5; ANX A5; ANX5; ANXA5; Calphobindin I; CBP I; CBP-I; Endonexin II; ENX 2; ENX2; Lipocortin V; PAP I; Placental anticoagulant protein I; PAP-I; Placental anticoagulant protein 4; PP 4; PP4; Thromboplastin inhibitor; VAC alpha; Vascular anticoagulant alpha; Vascular anticoagulant-alpha; ANXA5_HUMAN; Anchorin CII; Annexin-5; VAC-alpha. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg  0.2ml/200μg       

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human 

产品类型  一抗    

研究领域  细胞凋亡  

蛋白分子量  predicted molecular weight: 36kDa 

性    状  Lyophilized or Liquid 

免 疫 原  recombind human Annexin V 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

重组膜粘连蛋白5抗体产品介绍 The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008].

Function : Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.

Subunit : Interacts with PDCD6.

DISEASE : Defects in ANXA5 are associated with susceptibility to pregnancy loss, recurrent, type 3 (RPRGL3) [MIM:614391]. A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions.

Similarity : Belongs to the annexin family. 

Contains 4 annexin repeats. 

Database links : UniProtKB/Swiss-Prot: P20073.3

Annexin-V是一种分子量为35～36KD的Ca2+依赖性磷脂结合蛋白，能与PS高亲和力特异性结合。

将Annexin-V进行荧光素（FITC、PE）或biotin标记，以标记了的Annexin-V作为荧光探针，利用流式细胞仪或荧光显微镜可检测细胞凋亡的发生。

其意义：检测早期凋亡的细胞，同时可区分活细胞和坏死细胞磷脂酰丝氨酸（Phosphatidylserine，PS）正常位于细胞膜的内侧，但在细胞凋亡的早期，PS可从细胞膜的内侧翻转到细胞膜的表面，暴露在细胞外环境中。

碘化丙啶（propidine iodide，PI）是一种核酸染料，它不能透过完整的细胞膜，但在凋亡中晚期的细胞和死细胞，PI能够透过细胞膜而使细胞核红染。因此将Annexin-V与PI匹配使用，就可以将凋亡早晚期的细胞以及死细胞区分开来。