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活化转录因子3抗体

供货周期：现货

品牌： Abcam

规格： 0.1ml/100μg

货号：

CAS号：

报价： ¥1

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产品介绍

活化转录因子3抗体英文名称 Anti-ATF3

中文名称活化转录因子3抗体

别名 Activating transcription factor 3; ATF3deltaZip2; ATF3deltaZip2c; ATF3deltaZip3; cAMP dependent transcription factor ATF3; Cyclic AMP dependent transcription factor ATF3; Cyclic AMP-dependent transcription factor ATF-3; TF3_HUMAN; cAMP-dependent transcription factor ATF-3; cyclic AMP-dependent transcription factor ATF-3 isoform 1.

浓度 1mg/1ml

规格 0.1ml/100μg 0.2ml/200μg

公司专售活化转录因子3抗体、肿瘤抑制/凋亡抗体、信号分子抗体、结构蛋白抗体、磷酸化特异抗体、融合蛋白tag抗体、非哺乳动物蛋白抗体、细胞周期蛋白抗体、转录调节蛋白抗体、类固醇受体抗体、膜受体抗体、亚细胞标记抗体、同源结构域蛋白抗体、运输蛋白抗体、生长因子和激素抗体、神经生物抗体、激酶和磷酸化抗体、GDP/GTP结合蛋白抗体、合成降解蛋白抗体、离子通道抗体、淋巴细胞信号抗体、细胞粘附因子抗体、流式抗体等抗体种类，价格合理，品质有保障！

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow

产品类型一抗

研究领域肿瘤细胞生物免疫学信号转导细胞凋亡转录调节因子

蛋白分子量 predicted molecular weight: 20kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human ATF3 C-terminus

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

活化转录因子3抗体产品介绍 This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011].

Function : This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.

Subunit : Binds DNA as a homodimer or a heterodimer.

Subcellular Location : Nucleus.

Similarity : Belongs to the bZIP family. ATF subfamily.

Contains 1 bZIP domain.

Database links : UniProtKB/Swiss-Prot: P18847

转录调节因子（Transcriptin Regulators）

在纯化抗体时需要控制好几个指标，包括纯度、含量及抗体的抗原结合活性。

纯度：在实验的任何阶段，确定抗体溶液纯度的最简单方法是取一部分样本进行SDS-PAGE电泳。凝胶可用考马斯亮蓝染色(灵敏度为0.1—0.5ug/带)或银染(灵敏度1～l0ug/带)。

定量：如果抗体还不纯，有一个快捷的定量方法，即通过SDS-PAGE电泳分离出轻、重链，然后和已知的标准染色带比较。如果需要分析许多样本，用免疫测定法对抗体定量较容易。如果抗体是经过纯化的，可通过测蛋白总量代替上述两种方法，有一简单的方法，即紫外吸收法。活化转录因子3抗体的量可通过测280nm处的吸收值来测(10D大致相当于0.75mg/m1的纯化抗体)。

抗原结合活性：一般说来，纯化方法不会引起抗原结合活性的改变。用蛋白G或蛋白A树脂很少导致抗体活性丧失。然而，如果最终抗体产物的作用不如原来所预料的好，检测抗体纯化过程所丢失的活性就极为重要。用一系列滴定法比较纯化的抗体和其原材料的活性，以标定每一步中的总抗体量，这将有助于较好的估计通过纯化所丢失的活性。