氢钾ATP酶通道蛋白抗体

供货周期：现货

品牌： Abcam

规格： 0.1ml/100μg

货号：

CAS号：

报价： ¥1

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产品介绍

氢钾ATP酶通道蛋白抗体英文名称 Anti-ATP4B

中文名称氢钾ATP酶通道蛋白抗体

别名 Gastric H(+)/K(+) ATPase subunit beta; H+K+ ATPase; ATP4B; ATP6B; ATPase H+/K+ exchanging beta polypeptide; ATPase H+/K+ transporting beta polypeptide;Gastric H K ATPase catalytic subunit; Gastric H+/K+ ATPase beta subunit; Gastric hydrogen potassium ATPase; Gastric hydrogen potassium ATPase beta; Hydrogen/potassium exchanging ATPase 4B; Potassium transporting ATPase beta chain; Potassium transporting ATPase subunit beta; Proton pump; Proton pump beta chain; ATP4B_HUMAN.

浓度 1mg/1ml

规格 0.1ml/100μg 0.2ml/200μg

公司专售氢钾ATP酶通道蛋白抗体、肿瘤抑制/凋亡抗体、信号分子抗体、结构蛋白抗体、磷酸化特异抗体、融合蛋白tag抗体、非哺乳动物蛋白抗体、细胞周期蛋白抗体、转录调节蛋白抗体、类固醇受体抗体、膜受体抗体、亚细胞标记抗体、同源结构域蛋白抗体、运输蛋白抗体、生长因子和激素抗体、神经生物抗体、激酶和磷酸化抗体、GDP/GTP结合蛋白抗体、合成降解蛋白抗体、离子通道抗体、淋巴细胞信号抗体、细胞粘附因子抗体、流式抗体等抗体种类，价格合理，品质有保障！

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,

产品类型一抗

研究领域细胞生物免疫学转录调节因子

蛋白分子量 predicted molecular weight: 33kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human ATP4B C-terminus

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

氢钾ATP酶通道蛋白抗体保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P type cation transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta subunit which may play a role in maintaining the structural and functional integrity of the complex.

Function : Required for stabilization and maturation of the catalytic proton pump alpha subunit and may also involved in cell adhesion and establishing epithelial cell polarity.

Subunit : Composed of two subunits: alpha (catalytic) and beta.

Subcellular Location : Cell membrane

Tissue Specificity : Expressed at highest levels in heart, skeletal

Similarity : Belongs to the X(+)/potassium ATPases subunit beta family.

Database links : UniProtKB/Swiss-Prot: P51164.1

在纯化抗体时需要控制好几个指标，包括纯度、含量及抗体的抗原结合活性。

纯度：在实验的任何阶段，确定抗体溶液纯度的最简单方法是取一部分样本进行SDS-PAGE电泳。凝胶可用考马斯亮蓝染色(灵敏度为0.1—0.5ug/带)或银染(灵敏度1～l0ug/带)。

定量：如果抗体还不纯，有一个快捷的定量方法，即通过SDS-PAGE电泳分离出轻、重链，然后和已知的标准染色带比较。如果需要分析许多样本，用免疫测定法对抗体定量较容易。如果抗体是经过纯化的，可通过测蛋白总量代替上述两种方法，有一简单的方法，即紫外吸收法。氢钾ATP酶通道蛋白抗体的量可通过测280nm处的吸收值来测(10D大致相当于0.75mg/m1的纯化抗体)。

抗原结合活性：一般说来，纯化方法不会引起抗原结合活性的改变。用蛋白G或蛋白A树脂很少导致抗体活性丧失。然而，如果最终抗体产物的作用不如原来所预料的好，检测抗体纯化过程所丢失的活性就极为重要。用一系列滴定法比较纯化的抗体和其原材料的活性，以标定每一步中的总抗体量，这将有助于较好的估计通过纯化所丢失的活性。