l 设定:界面用来输入使计算机正确记录测量结果的所有细节,以便于用正确的物理量单位记录,用需要的单位表达呼吸率,如果有必要,可统一生物量单位。所有在此输入的细节都可以传到最终的计算结果报告中。在设定过程中,池的容量(封闭池)及流动速率,电极位置(流式呼吸测量),实验温度,记录过程中曲线的滚动速度和打印所记录的屏幕的打印输出指令(如果需要硬拷贝)都需要输入。大多数这些设定都不会随着实验不同而改变,所以需要输入那些改变的设定 l 记录:鼠标点击“Start”按钮开始记录过程,随后屏幕上滚屏显示记录的曲线。如果在记录过程中需要加入底物,抑制剂或其他溶剂,这个变化出现的时刻可以用一个小游标表示,同时可以通过下拉菜单记录添加物的属性。在记录过程结束后,数据以数据文件的形式保存,并可按要求直接分析 l 分析:屏幕中可以再次显示数据文件、记录曲线以及事件游标。如果纪录超过了整屏的宽度,可以按照用户要求缩小到适合整屏的宽度。也可以沿着长长的记录曲线滚动屏幕。可以拖动选择条来选定曲线的特定部分。因而,如果在添加溶液后同时显示对照曲线和呼吸改变后的曲线,可以首先拖动选择条到对照频率然后再到改变后的频率曲线
Respiration of mitochondria Cell、oxidative enzymes(线粒体细胞、氧化酶呼吸测试)
配置一:782溶氧仪(2通道)—RC300/RC350呼吸测量池—1302电极(1-2个)
配置二:929溶氧仪(6通道)——RC650呼吸测量池——1302电极(6个)
PO2 of blood(血液中氧分压测试)
782溶氧仪(1-2通道)—MC100呼吸测量池—1302电极(1-2个)
Oxygen content of blood(血液中氧含量测试)
782溶氧仪(1-2通道)—TC500 Tucker呼吸测量池—1302电极(1-2个)
PO2 monitoring in-line在线氧分压测试
782溶氧仪,或929溶氧仪
1302电极
MC100呼吸测量池用于小量样品
FC100呼吸测量池用于流通测试
PO2 of sediments氧分压沉积物测试
782溶氧仪,或929溶氧仪
针或微电极
In Vitro organ respiration(离体器官呼吸测试)
782溶氧仪(2通道);或选择929溶氧仪(6通道)可监测5个器官
在线监测FC100呼吸测量池(2个);或小量MC100呼吸测量池(2个)
1302电极(2个或6个)
Respiration of aquatic organisms (closed cell method) 水中生物呼吸测试(闭孔法)
782溶氧仪,或929溶氧仪
1302电极
根据用于复制的生物体大小选择合适的呼吸测量池,如MT200,MT200A,RC300,
RC350,RC400,EH100
Respiration of aquatic organisms (flowcell method) 水中生物呼吸测试(流通法)
782溶氧仪,或929溶氧仪
1302电极
MC100呼吸测量池用于小量样品
FC100呼吸测量池用于流通测试
Photosynthesis of aquatic plants(水生植物的光合作用测试)
782溶氧仪,或929溶氧仪
1302电极
可选择RC300、RC350、RC400、EH100呼吸测量池
研究领域: 1. Mitochondrial respiration Mitochondrial dysfunction is responsible for >30 pathological conditions. Some of which can be detected by changes to oxidative phosphorylation patterns e.g. by changes in P/O ratios. 2. Apoptosis Since mitochondria are highly implicated in apoptosis, changes to cellular respiratory metabolism can be used as an important indicator of apoptosis mechanisms. 3. Cytotoxicity The potential role of respirometry in cytotoxicity research on drug discovery, development of novel bacteriocides etc is only just being realized. Almost any drug-induced change to a metabolic pathway will theoretically result in a change in energy metabolism. Respirometry can therefore be used, for instance, to establish time/dosage responses over very short timeframes. 4. New product toxicity In order to obtain a production license, all chemical manufacturers have to establish the toxicity of any new chemical to be manufactured for bulk use, using the OECD 209 test. This measures the respiration inhibition of activated sludge bacteria when exposed to a range of concentrations of the chemical. 5. Environmental toxicity Present tests of toxicity of effluents discharged to rivers and oceans, use LC50 tests on target aquatic organisms. A more useful test is the EC50 test of respiration inhibition. This is a rigorous test and has the advantage that the test organisms do not need to be killed.