SPEX GENO 96孔格式高通量破坏酵母

• Geno ApplicationnoteSP018 Geno High thru put yeast disruption in a 96-Well Format GENO 96孔格式高通量破坏酵母 Results and Discussion The efficiency of yeast disruption is dependent upon the size of the glass beads and the duration of the grinding. Figures 1-3 demonstrate the efficiency of cell disruption with 425-600 mesh glass beads at 5 and 10 minutes. Lower mesh glass beads were less effective in cracking cells with the 106 mesh beads being particularly ineffective. It is concluded that 425-600 mesh glass beads are most suited for disrupting Saccharomyces. The design of the Geno/Grinder is also believed to be a factor in the disruption process. Standard bead mills adapted to microwell plates are modeled after "paint shakers" and move the plates in a "figure eight" motion. This motion is not believed to lead to uniform cell disruption. The Geno/Grinder is designed to effectively disrupt cellular materials by oscillating the plate vertically. This motion allows glass beads to impact the cells more directly than standard mills where beads impact the well walls in addition to the cells.