采用SPEX冷冻研磨机进行研磨-样品蛋白质（鱼）

• ELSEVIER ScienceDirect www.elsevier.com/locate/yabio Analytical Biochemistry 368 (2007) 33–38 An assay for thiaminase I in complex biological samples 重组生物样品中硫胺酶I的活性检测-美国康奈尔大学 本实验所涉及的样品（鱼）经干燥后，在SPEX 6850冷冻研磨机内进行研磨，所得粉末即用于酶的提取． Jeremiah W. Hanes a, Clifford E. Kraft b, Tadhg P. Begley a,* a Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA b Department of Natural Resources, Cornell University, Ithaca, NY 14853, USA R eceived 8 November 2006 Available online 7 June 2007 Abstract An alternative method for measuring thiaminase I activity in complex samples is described. This assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, resulting in a large decrease in absorbance at 411 nm. This new assay is simple and sensitive, and it requires only readily available chemicals and a visible region spectrophotometer. In addition, the assay is optimized for high-throughput analysis in a 96-well format with complex biological samples. Published by Elsevier Inc.