人丝胺酸丙酮酸氨基转移酶(AGXT) ELISA kit

产品类型:ELISA Kit

产品名称:人丝胺酸丙酮酸氨基转移酶(AGXT) ELISA kit

英文名称:Human Serine--pyruvate aminotransferase(AGXT) ELISA kit

别名: AGT, AGT1, AGXT1, PH1, SPAT, SPT, TLH6, L-alanine: glyoxylate aminotransferase 1|hepatic peroxisomal alanine:glyoxylate aminotransferase|serine-pyruvate aminotransferase|serine:pyruvate aminotransfe 

种属: Human 

待测物名称: alanine-glyoxylate aminotransferase 

缩写: AGXT 

检测范围: 31.25 pg/ml-2000 pg/ml 

灵敏度: 7.81 pg/ml 

反应时间: 1-5h 

所需样本体积: 50-100ul 

检测波长: 450 nm

实验原理

用纯化的抗体包被微孔板，制成固相载体，往包被抗丝胺酸丙酮酸氨基转移酶(AGXT)抗体的微孔中依次加入标本或标准品、生物素化的丝胺酸丙酮酸氨基转移酶(AGXT)抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的丝胺酸丙酮酸氨基转移酶(AGXT)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

试剂盒组成及试剂配制

1. 酶联板（Assay plate ）：一块（96孔）。

2. 标准品（Standard）：2瓶（冻干品）。

3. 样品稀释液（Sample Diluent）：1×20ml/瓶。

4. 生物素标记抗体稀释液（Biotin-antibody Diluent）：1×10ml/瓶。

5. 辣根过氧化物酶标记亲和素稀释液 （HRP-avidin Diluent）：1×10ml/瓶。

6. 生物素标记抗体（Biotin-antibody）：1×120μl/瓶（1：100）

7. 辣根过氧化物酶标记亲和素（HRP-avidin）：1×120μl/瓶（1：100）

8. 底物溶液（TMB Substrate）：1×10ml/瓶。

9. 浓洗涤液（Wash Buffer）：1×20ml/瓶，使用时每瓶用蒸馏水稀释25倍。

10. 终止液（Stop Solution）：1×10ml/瓶（2N H2SO4）。

人丝胺酸丙酮酸氨基转移酶(AGXT) ELISA kit免费代测。

相关产品：

人丝胺酸丙酮酸氨基转移酶(AGXT) ELISA kit Human Serine--pyruvate aminotransferase(AGXT) ELISA kit

小鼠丝胺酸丙酮酸氨基转移酶(AGXT) ELISA kit Mouse Serine--pyruvate aminotransferase(AGXT) ELISA kit

Human Adenosylhomocysteinase(AHCY) ELISA kit Human Adenosylhomocysteinase(AHCY) ELISA kit

Human Protein AHNAK2(AHNAK2) ELISA kit Human Protein AHNAK2(AHNAK2) ELISA kit

人芳香烃受体(AhR)ELISA Kit Human aryl hydrocarbon receptor,AhR ELISA Kit

Rat Aryl hydrocarbon receptor(AHR) ELISA kit Rat Aryl hydrocarbon receptor(AHR) ELISA kit

Human Aryl hydrocarbon receptor repressor(AHRR) ELISA kit Human Aryl hydrocarbon receptor repressor(AHRR) ELISA kit

Mouse Activator of 90 kDa heat shock protein ATPase homolog 1(AHSA1) ELISA kit Mouse Activator of 90 kDa heat shock protein ATPase homolog 1(AHSA1) ELISA kit

Bovine Alpha-2-HS-glycoprotein(AHSG) ELISA kit Bovine Alpha-2-HS-glycoprotein(AHSG) ELISA kit

人胎球蛋白A ELISAkit human Fetuin A ELISA Kit

小鼠胎球蛋白A ELISAkit Mouse Fetuin A ELISA Kit

猪胎球蛋白A ELISA Kit Pig Fetuin A ELISA Kit

大鼠α2-HS糖蛋白(AHSG)ELISA kit Rat Alpha 2 Heremans Schmid Glycoprotein(AHSG) ELISA Kit

Human Activation-induced cytidine deaminase(AICDA) ELISA kit Human Activation-induced cytidine deaminase(AICDA) ELISA kit

Human Allograft inflammatory factor 1(AIF1) ELISA kit Human Allograft inflammatory factor 1(AIF1) ELISA kit

Mouse Allograft inflammatory factor 1(AIF1) ELISA kit Mouse Allograft inflammatory factor 1(AIF1) ELISA kit

Rat Allograft inflammatory factor 1(AIF1) ELISA kit Rat Allograft inflammatory factor 1(AIF1) ELISA kit

人凋亡诱导因子(AIF)ELISA Kit Human apoptosis inducing factor,AIF ELISA Kit

人A激酶PRKA锚定蛋白12(Akap12)ELISA Kit human A kinase PRKA anchor protein-gravin 12,Akap12 ELISA Kit

Human A-kinase anchor protein 4(AKAP4) ELISA kit Human A-kinase anchor protein 4(AKAP4) ELISA kit

人醛糖还原酶(AR)ELISA Kit Human Aldose Reductase(AR)ELISA Kit

Mouse Aldose reductase(AKR1B1) ELISA kit Mouse Aldose reductase(AKR1B1) ELISA kit

Rat Aldose reductase(AKR1B1) ELISA kit Rat Aldose reductase(AKR1B1) ELISA kit

Human Aldo-keto reductase family 1 member B10(AKR1B10) ELISA kit Human Aldo-keto reductase family 1 member B10(AKR1B10) ELISA kit

Human Aldo-keto reductase family 1 member C3(AKR1C3) ELISA kit Human Aldo-keto reductase family 1 member C3(AKR1C3) ELISA kit

人RACα丝氨酸/苏氨酸蛋白激酶(AKT1)ELISA kit Human RAC-alpha serine/threonine-protein kinase(AKT1) ELISA kit

小鼠蛋白激酶B(PKB)ELISA Kit Mouse protein kinase B,PKB ELISA Kit

大鼠蛋白激酶B(PKB/Akt) ELISA Kit Rat Protein Kinase B (PKB/Akt) ELISA Kit

Human Delta-aminolevulinic acid dehydratase(ALAD) ELISA kit Human Delta-aminolevulinic acid dehydratase(ALAD) ELISA kit

牛血清白蛋白(ALB)ELISA kit Bovine Serum albumin(ALB) ELISA kit

Dog Albumin ELISA Kit Dog Albumin ELISA Kit

Goat Albumin ELISA Kit Goat Albumin ELISA Kit

马白蛋白ELISA Kit Horse Albumin ELISA Kit

人尿微量白蛋白(MAU/ALB)ELISA kit Human microalbunminuria(MAU/ALB) ELISA kit 

小鼠白蛋白ELISA Kit Mouse albumin ELISA Kit

猪白蛋白ELISA Kit Pig Albumin ELISA Kit

兔白蛋白ELISA Kit Rabbit Albumin ELISA Kit

绵羊白蛋白ELISA Kit Sheep Albumin ELISA Kit

小鼠白细胞活化黏附因子(ALCAM)ELISA Kit Mouse Activated Leukocyte Cell Adhesion Molecule,ALCAM ELISA Kit

大鼠白细胞活化黏附因子(ALCAM)ELISA Kit Rat Activated Leukocyte Cell Adhesion Molecule,ALCAM ELISA Kit

Human Retinal dehydrogenase 1(ALDH1A1) ELISA kit Human Retinal dehydrogenase 1(ALDH1A1) ELISA kit

Human Aldehyde dehydrogenase family 1 member A3(ALDH1A3) ELISA kit Human Aldehyde dehydrogenase family 1 member A3(ALDH1A3) ELISA kit

小鼠乙醛脱氢酶,线粒体(ALDH2)ELISA kit Mouse Aldehyde dehydrogenase, mitochondrial(ALDH2) ELISA kit

大鼠乙醛脱氢酶(ALDH)ELISA Kit Rat aldehyde dehydrogenase,ALDH ELISA Kit

Human Succinate-semialdehyde dehydrogenase, mitochondrial(ALDH5A1) ELISA kit Human Succinate-semialdehyde dehydrogenase, mitochondrial(ALDH5A1) ELISA kit

人果糖二磷酸醛缩酶A(ALDOA) ELISA kit Human aldolase A, fructose-bisphosphate (ALDOA) ELISA kit