大鼠Agouti相关蛋白(AGRP)ELISA Kit

产品类型:ELISA Kit

产品名称:大鼠Agouti相关蛋白(AGRP)ELISA Kit

英文名称:Rat Agouti Related Protein,AGRP ELISA Kit

种属: Rat 

待测物名称: Agouti Related Protein,AGRP 

缩写: AGRP 

检测范围: 0.156 ng/ml-10 ng/ml 

灵敏度: 0.039 ng/ml 

反应时间: 1-5h 

所需样本体积: 50-100ul 

检测波长: 450 nm

实验原理

用纯化的抗体包被微孔板，制成固相载体，往包被抗Agouti相关蛋白(AGRP)抗体的微孔中依次加入标本或标准品、生物素化的Agouti相关蛋白(AGRP)抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Agouti相关蛋白(AGRP)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

试剂盒组成及试剂配制

1. 酶联板（Assay plate ）：一块（96孔）。

2. 标准品（Standard）：2瓶（冻干品）。

3. 样品稀释液（Sample Diluent）：1×20ml/瓶。

4. 生物素标记抗体稀释液（Biotin-antibody Diluent）：1×10ml/瓶。

5. 辣根过氧化物酶标记亲和素稀释液 （HRP-avidin Diluent）：1×10ml/瓶。

6. 生物素标记抗体（Biotin-antibody）：1×120μl/瓶（1：100）

7. 辣根过氧化物酶标记亲和素（HRP-avidin）：1×120μl/瓶（1：100）

8. 底物溶液（TMB Substrate）：1×10ml/瓶。

9. 浓洗涤液（Wash Buffer）：1×20ml/瓶，使用时每瓶用蒸馏水稀释25倍。

10. 终止液（Stop Solution）：1×10ml/瓶（2N H2SO4）。

大鼠Agouti相关蛋白(AGRP)ELISA Kit免费代测。

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