人溶血磷脂酸性磷酸酶6(ACP6) ELISA kit

产品类型:ELISA Kit

产品名称:人溶血磷脂酸性磷酸酶6(ACP6) ELISA kit

英文名称:Human Lysophosphatidic acid phosphatase type 6(ACP6) ELISA kit

别名: ACPL1, LPAP, PACPL1, acid phosphatase like 1|lysophosphatidic acid phosphatase 

种属: Human 

待测物名称: acid phosphatase 6, lysophosphatidic 

缩写: ACP6 

反应时间: 1-5h 

所需样本体积: 50-100ul 

检测波长: 450 nm

实验原理

用纯化的抗体包被微孔板，制成固相载体，往包被抗溶血磷脂酸性磷酸酶6(ACP6)抗体的微孔中依次加入标本或标准品、生物素化的溶血磷脂酸性磷酸酶6(ACP6)抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的溶血磷脂酸性磷酸酶6(ACP6)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

试剂盒组成及试剂配制

1. 酶联板（Assay plate ）：一块（96孔）。

2. 标准品（Standard）：2瓶（冻干品）。

3. 样品稀释液（Sample Diluent）：1×20ml/瓶。

4. 生物素标记抗体稀释液（Biotin-antibody Diluent）：1×10ml/瓶。

5. 辣根过氧化物酶标记亲和素稀释液 （HRP-avidin Diluent）：1×10ml/瓶。

6. 生物素标记抗体（Biotin-antibody）：1×120μl/瓶（1：100）

7. 辣根过氧化物酶标记亲和素（HRP-avidin）：1×120μl/瓶（1：100）

8. 底物溶液（TMB Substrate）：1×10ml/瓶。

9. 浓洗涤液（Wash Buffer）：1×20ml/瓶，使用时每瓶用蒸馏水稀释25倍。

10. 终止液（Stop Solution）：1×10ml/瓶（2N H2SO4）。

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Human Anterior gradient protein 3 homolog(AGR3) ELISA kit Human Anterior gradient protein 3 homolog(AGR3) ELISA kit

人聚集蛋白(Agrin)ELISA Kit Human Agrin ELISA Kit

Mouse Agrin(AGRN) ELISA kit Mouse Agrin(AGRN) ELISA kit

Rat Agrin(AGRN) ELISA kit Rat Agrin(AGRN) ELISA kit

Human Agouti Related Protein,AGRP ELISA Kit Human Agouti Related Protein,AGRP ELISA Kit

Mouse Agouti-related protein(AGRP) ELISA kit Mouse Agouti-related protein(AGRP) ELISA kit

大鼠Agouti相关蛋白(AGRP)ELISA Kit Rat Agouti Related Protein,AGRP ELISA Kit

Horse Angiotensinogen(AGT) ELISA kit Horse Angiotensinogen(AGT) ELISA kit

人血管紧张素原(aGT)ELISA Kit Human angiotensinogen (aGT) ELISA Kit

小鼠血管紧张素原(aGT)ELISA Kit Mouse angiotensinogen (aGT) ELISA Kit

大鼠血管紧张素原(aGT)ELISA Kit Rat angiotensinogen (aGT) ELISA Kit

人血管紧张素Ⅱ受体1(ANGⅡR-1)ELISA Kit Human angiotensin Ⅱ receptor 1,ANGⅡR-1 ELISA Kit

大鼠血管紧张素Ⅱ受体1(AT1R)ELISA Kit Rat angiotensin Ⅱ receptor type 1(AT1R)ELISA Kit