小鼠解整合素样金属蛋白酶12(ADAM12) ELISA kit

产品类型:ELISA Kit

产品名称:Mouse Disintegrin and metalloproteinase domain-containing protein 12(ADAM12) ELISA kit

英文名称:Mouse Disintegrin and metalloproteinase domain-containing protein 12(ADAM12) ELISA kit

别名: RP11-295J3.5, MCMP, MCMPMltna, MLTN, MLTNA, a disintegrin and metalloproteinase domain 12 (meltrin alpha)|meltrin alpha 

种属: Mouse 

待测物名称: ADAM metallopeptidase domain 12 

缩写: ADAM12 

蛋白功能1: Cell Adhesion 

蛋白功能3: Cell adhesion 

检测范围: 0.156 ng/ml-10 ng/ml 

灵敏度: 0.039 ng/ml 

反应时间: 1-5h 

所需样本体积: 50-100ul 

检测波长: 450 nm

实验原理

用纯化的抗体包被微孔板，制成固相载体，往包被抗解整合素样金属蛋白酶12(ADAM12)抗体的微孔中依次加入标本或标准品、生物素化的解整合素样金属蛋白酶12(ADAM12)抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的解整合素样金属蛋白酶12(ADAM12)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

试剂盒组成及试剂配制

1. 酶联板（Assay plate ）：一块（96孔）。

2. 标准品（Standard）：2瓶（冻干品）。

3. 样品稀释液（Sample Diluent）：1×20ml/瓶。

4. 生物素标记抗体稀释液（Biotin-antibody Diluent）：1×10ml/瓶。

5. 辣根过氧化物酶标记亲和素稀释液 （HRP-avidin Diluent）：1×10ml/瓶。

6. 生物素标记抗体（Biotin-antibody）：1×120μl/瓶（1：100）

7. 辣根过氧化物酶标记亲和素（HRP-avidin）：1×120μl/瓶（1：100）

8. 底物溶液（TMB Substrate）：1×10ml/瓶。

9. 浓洗涤液（Wash Buffer）：1×20ml/瓶，使用时每瓶用蒸馏水稀释25倍。

10. 终止液（Stop Solution）：1×10ml/瓶（2N H2SO4）。

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