Catalogue Number | CYT-027 | ||
Synonyms | Fibroblast Growth Factor 15, FGF19. | ||
Introduction | FGF-15 takes part in the suppression of bile acid biosynthesis by down-regulation of CYP7A1 expression. FGF-15 is expressed in the developing brain. | ||
Description | FGF-15 Protein is 23.78 kDa protein containing 203 amino acid residues including a 10 a.a. N-Terminal His-tag. | ||
Source | E. coli | ||
Physical Appearance | Filtered White lyophilized (freeze-dried) powder. | ||
Formulation | FGF-15 was filtered (0.4µm) and lyophilized from 0.5 mg/ml supplied in 20mM TRIS and 20mM NaCl, pH 8.0. | ||
Solubility | It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture. | ||
Stability | Store lyophilized FGF-15 at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted FGF-15 can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. | ||
Amino Acid Sequence | MKHHHHHHAS RPLAQQSQSV SDEDPLFLYG WGKITRLQYL YSAGPYVSNC FLRIRSDGSV DCEEDQNERN LLEFRAVALK TIAIKDVSSV RYLCMSADGK IYGLIRYSEE DCTFREEMDC LGYNQYRSMK HHLHIIFIQA KPREQLQDQK PSNFIPVFHR SFFETGDQLR SKMFSLPLES DSMDPFRMVE DVDHLVKSPS FQK. | ||
Applications | Western blotting | ||
Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||
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