Recombinant Human RO-52/SS-A(ROSS52)

RO 52 Human
RO-52 (SS-A) Human Recombinant
Catalog No. PRO-328

Synonyms:
52 kDa Ro protein, Sjoegren syndrome type A antigen, SS-A, Ro(SS-A), 52 kDa ribonucleoprotein autoantigen Ro/SS-A, Tripartite motif-containing protein 21, RING finger protein 81, TRIM21, RNF81, RO52, SSA1, SSA, RO-52.

Introduction:
TRIM21 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The 52 kDa Ro protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. Ro/SSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Ribonucleoprotein particle is composed of a single polypeptide and one of four small RNA molecules. The RoSSA is present in all mammalian cells studied but has no known function. At least 2 isoforms are present in nucleated and red blood cells, and tissue specific differences in Ro/SSA proteins were identified.

Description:
RO-52 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 52kDa.
The RO-52 is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered clear solution.

Formulation:
The protein solution contains 6M Urea, 500mM NaCl and 10mM Tris pH8.0.

Stability:
Lyophilized RO-52 although stable at 4°C for 3 weeks, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.

Purity:
Greater than 90.0% as determined by both:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Protein content:
Protein quantitation was carried out by using 0.25 - 2.0 mg/ml Bradford assay vs. BSA.

Western Blot:
Strongly reactive with human anti RO/SS-A.

Amino Acid Sequence:
MASAARLTMMWEEVTCPICLDPFVEPVSIECGHSFCQECISQVGKGG
GSVCAVCRQRFLLKNLRPNRQLANMVNNLKEISQEAREGTQGERCA
VHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKL
QVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNF
LVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRC
HSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTC
AVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLG
AQHFHSGKHYWEVDVTGKEAWDLGVCRDSVRRKGHFLLSSKSGFWT
IWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDHGS
LIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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