Introduction:
Mammalian cells undergoing apoptosis express high levels of APG5L (or ASP) protein relatively late in the apoptotic process. APG5L protein contains a high number of acidic residues, a potential glycosylation site, and potential casein kinase II, protein kinase C, and tyrosine kinase sites.
Description:
Autophagy Protein 5 Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain having a molecular weight of 31.6 kDa.
APG5 contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.
Formulation:
APG5 at a concentration of 0.1mg/ml containing 10mM Tris, pH 8.0, 0.01% Triton X-100 and 0.002% NaN3 and 10mM DTT.
Stability:
APG5 although stable at 4°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Applications:
• ELISA
• MS
• Western Blotting
Purity:
Greater than 95.0% as determined by SDS-PAGE.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.