重组多磷酸激酶(Polyphospate Kinase/PPK)

PPK
Polyphosphate Kinase Recombinant
Catalog No. ENZ-266

Synonyms:
Polyphosphate kinase, EC 2.7.4.1, Polyphosphoric acid kinase, ATP-polyphosphate phosphotransferase.

Introduction:
PPK catalyzes the reversible transfer of phosphate between polyphosphate and ATP. The phosphorylation of ADP to ATP by polyphosphate kinase is by a processive mechanism; the phosphorylation occurs without release of the polymer from the enzyme prior to termination of the reaction.

Description:
Polyphosphate Kinase purified circa 10 fold . Free of all Polyphosphate Glucokinase activity.
Native MW = 83 kD (monomer)

Source:
Propionibacterium shermanii

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein was lyophilized from PPK solution containing 11.54 U/ml of PPK activity, 10.3 mg/ml total protein, 100mM potassium phosphate pH 6.8 and 25mM sodium polyphosphate.

Solubility:
The contents of the vial can be reconstituted in 1-10 ml deionized water.

Stability:
Lyophilized Polyphosphate kinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PPK should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Definition of Activity Units:
The amount of Polyphosphate kinase required to convert 1 µmole ADP to ATP per minute at pH 7.5, using polyphosphate as phosphate donor.

Specific Activity:
1.12 U/mg.

Required Cofactors:
The reaction requires Mg2+.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.