MMLV-RT

MMLV RT
Moloney Murine Leukemia Virus Reverse Trancscriptase Recombinant
Catalog No. ENZ-310

Description:
MMLV (Moloney Murine Leukemia Virus) Reverse Transcriptase is a DNA polymerase that synthesizes a complementary DNA strands from single-stranded RNA, DNA, or an RNA-DNA hybrid as a template. This recombinant enzyme was purified from E.coli, which carried a modified MMLV-RT gene. Compared to AMV Reverse Transcriptase, this enzyme has a much weaker 5' - 3' ribonuclease H activity, which allows the syntesis of longer cDNAs (>7kb).

Source:
Recombinant E. coli strain.

Physical Appearance:
Sterile Filtered clear solution (200 U/µl).

Concentration:
200 units/ml.

Formulation:
50mM Tris-HCl, 0.1M NaCl, 0.1% Triton X-100, 2mM DTT, 0.1mM EDTA and 50% glycerol.

Stability:
Stable for 5 days at 10°C, for longer period of time store at -20°C.
Please prevent freeze-thaw cycles.

Applications:
1. 1st strand cDNA synthesis.
2. Primer extension.
3. RT-PCR.

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 1nmol of deoxyribonucleotide into acid-insoluble forms in 10 minutes at 37oC, using poly(A)-oligo(dT)12-18 as the template-primer.

Standard cDNA Synthesis Conditions:
50mM Tris-HCl (pH8.3), 75mM KCl, 3mM MgCl2, 10mM DTT, 1.0mM each dATP, dGTP, dCTP, and dTTP, 0.2 mg radom hexamer,1-5mg RNA, 200units M-MLV RT. The reaction volume was 20ml and the incubation was 45 min at 42oC.

5X Reaction Buffer:
250mM Tris-HCl (pH8.3), 375mM KCl, 15mM MgCl2, and 50mM DTT.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.