Dereplication-guided isolation of depsides thielavins S–T and lecanorins D–F from the endophytic fungus Setophoma sp. through Biotage Isolera耐士科技
2015-02-13 10:19
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Dereplication methodology using UHPLC–DAD–QTOFMS was applied during the metabolic profiling investigation of the endophyte Setophoma sp., a fungus isolated from symptomless guava fruits. The approach performed allowed a fast analysis of the microbial secondary metabolites. From this fungus, seven highly C-alkylated depsides were isolated and identified as polyketides thielavins S, T, U and V and lecanorins D, E and F. Their structures were elucidated through spectroscopic methods including NMR, HRMS and especially with assistance of HRMS/MS experiments. The compounds were tested for quorum sensing regulation activity in the virulence gene expression of Staphylococcus aureus, but no inhibitory effect was detected. Nevertheless, moderate antibacterial activity was encountered in three of tested depsides, particularly with thielavin T, whose MIC was 6.25 lg/mL against S. aureus.
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ereplicationguidedisolationofdepsidesthielavinsandlecanorinsfromtheendophyticfungusetophomasp.throughiotagesolera耐士科技.pdf
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耐士科技作为Biotage中国区总代理,以优质的服务为您提供Biotage全系产品以及相关的技术服务。
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