通过Biotage Isolera快速纯化液相制备色谱用蔗糖或葡萄糖作为糖供体磷酸进行区域选择性和立体选择性糖基化反应by耐士科技
2015-02-12 17:43
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Previously it has been shown that glycerol can be regioselectively glucosylated by sucrose phosphorylase from Leuconostoc mesenteroides to form 2-O--d-glucopyranosyl glycerol.A series of compounds related to glycerol were investigated by us to determine the scope of the -glucosylation reaction of sucrose phosphorylase. Both sucrose
and glucose 1-phosphate (G1P) were applied as glucosyl donor. Mono-alcohols were not accepted as substrates but several 1,2-diols were readily glucosylated, proving that the vicinal diol unit is crucial for activity. The smallest substrate that was accepted for glucosylation appeared to be ethylene glycol,which was converted to the monoglucoside for 69%. Using high acceptor and donor concentrations (up to 2.5 M), sucrose or G1P hydrolysis (with H2O being the ‘acceptor’) can be minimised. In the study cited above, a preference for glucosylation of glycerol on the 2-position has been observed. For 1,2-propanediol however, the regiochemistry appeared to be dependent on the configuration of the substrate. The (R)-enantiomer was preferentialy glucosylated on its 1-position (ratio 2.5:1), whereas the 2-glucoside is the major product for (S)-1,2-propanediol (1:4.1). d.e.ps of 71–83% were observed with a preference for the (S)-enantiomer of the glucosides of 1,2-propanediol and 1,2-butanediol and the (R)-enantiomer of the glucoside of 3-methoxy-1,2-propanediol. This is the first example of stereoselective glucosylation of a non-natural substrate by sucrose phosphorylase. 3-Amino-1,2-propanediol, 3-chloro-1,2-propanediol, 1-thioglycerol and glyceraldehyde were not accepted as substrates.
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通过iotagesolera快速纯化液相制备色谱用蔗糖或葡萄糖作为糖供体磷酸进行区域选择性和立体选择性糖基化反应by耐士科技.pdf
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We herein report several highly active catalyst systems with thiadiazolidine 1-oxides as ligands for palladium in the MizorokieHeck reaction. Excellent yields of stilbenes derived from aryl iodides and bromides have been achieved using as little as 0.00002 mol % catalyst. The ligand/palladiumsystem can be stored as a stock solution open to air at room temperature with no observable loss of activity for a period of several months.
耐士科技作为Biotage中国区总代理,以优质的服务为您提供Biotage全系产品以及相关的技术服务。1、开机前确认自动收集机械手臂下无障碍物,试管高度低于机械手臂收集针
2、运行前确认所用流动相充足,废液瓶剩余空间充足
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1.开机,按屏幕左下方黑色电源键
2.进入开机界面后,点击Chemistry进入操作界面
3.在”Method”页面中设定好相关参数
耐士科技作为Biotage中国区总代理,以优质的服务为您提供Biotage全系产品以及相关的技术服务。
操作步骤:
1.开机,按屏幕左下方黑色电源键
2.进入开机界面后,点击
Organic Synthesis
进入编辑界面