NEPA21高效基因转染系统·CUY21SC /CUY21EDIT活体电转染仪
CUY21系列的方波电转染仪是Nepa Gene公司的老一代产品,被公认为是最专业的活体转基因仪器,并可以用于动物细胞转染,在研究领域中久负盛名。该产品已被数百篇文献引用,其中不乏高水平杂志的文章,如Nature、Cell、PNAS、Genes & Dvelopment等。
2011年,Nepa推出新一代全能型NEPA21高效基因转染系统。其特点有:
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全新设计的电转程序,
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特别适用于难转染细胞、离体组织或动物活体的转染,
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高转染效率、高细胞存活率
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电转程序各项参数可见、可调,适用性广
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不需要特殊的转染试剂盒辅助,运维成本低
部分参考文献:
细胞转染仪(NEPA21、CUY21系列)
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Barnabe-Heider et al. Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation. Nature Methods, 2008 Feb;5(2):189-96.
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Shibata MA, et al. Combination therapy with short interfering RNA vectors against VEGF-C and VEGF-A suppresses lymph node and lung metastasis in a mouse immunocompetent mammary cancer model. Cancer Gene Ther. 2008 Dec;15(12):776-86.
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Limura T and Pourquie O. Collinear activation of Hoxb genes during gastrulation is linked to mesoderm cell ingression. Nature, 2006 Aug 3;442(7102):568-71.
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Sanada K and Tsai LH. G Protein betagamma Subunits and AGS3 Control Spindle Orientation and Asymmetric Cell Fate of Cerebral Cortical Progenitors. Cell, 2005 Jul 15;122(1):119-31
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Ladher RK et al. FGF8 initiates inner ear induction in chick and mouse. Genes and Development, 2005 Mar 1;19(5):603-13.
细胞融合仪(ECFG21、LF201系列)
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Yin XJ, et al. Generation of cloned transgenic cats expressing red fluorescence protein. Biology of Reproduction. 2008 Mar;78(3):425-31.
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Egli D, et al. Developmental reprogramming after chromosome transfer into mitotic mouse zygotes. Nature. 2007 Jun 7;447(7145):679-85.
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Song K and Lee E. Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos. Journal of veterinary science. 2007 Mar;8(1):81-7.
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Sato A, et al. Gene therapy for progeny of mito-mice carrying pathogenic mtDNA by nuclear transplantation. Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16765-70.
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Hochedlinger K, et al. Reprogramming of a melanoma genome by nuclear transplantation. Genes & Development. 2004 Aug 1;18(15):1875-85.