Prodigiosin/灵菌红素的PubChem识别号5351169灵菌红素         CAS 号. 82-89-3灵菌红素是由若干种细菌特别是粘质沙雷菌产生的一种红色吡咯色素。灵菌红素有广谱的生物学活性，抗真菌、肿瘤细胞系和疟疾。2007年发现它是一种免疫抑制剂。近来灵菌红素的作用方式受到大量的关注，作为一种诱导剂通过活化半胱天冬酶诱导人原发性癌细胞的凋亡，并通过转化β生长因子受体途径诱导p21WAF1/CIP1表达，通过作用3β糖原合成酶激酶活化N-乙酰-β-葡萄糖苷酶。Prodigiosin  Prodigiosin is an intensely red pyrrole pigment produced by several bacteria, most notably, Serratia marcescens. Prodigiosin has a broad biological profile with activity against fungi, tumor cell lines and malaria. It was shown to be an immunosuppressant in 2007. The mode of action of prodigiosin has recently received considerable attention as an inducer of apoptosis in human primary cancer cells via caspase activation. Prodigiosin also acts as an inducer of p21WAF1/CIP1 expression via transforming growth factor-beta receptor pathway, and of NAG-1activation by acting on glycogen synthase kinase-3beta. CAS # : 82-89-3Molecular Formula : C20H25N3OMolecular Weight : 323.4Source : Serratia marcescens MST-AS5330Appearance : Dark red solidPurity : > 95%Long Term Storage : -20°CSolubility : Soluble in ethanol, methanol, DMF or DMSO. Limited water solubility References1. Seeing red: The story of prodigiosin. Bennett J.W. & Bentley R., Adv. Appl. Microbiol. 2000, 47, 1.2. Prodigiosin induces apoptosis of B and T cells from B-cell chronic lymphocytic leukemia. Campas C. etal., Leukemia 2003, 17, 746.3. Prodigiosin: a novel family of immunosuppressants with anti-cancer activity. Pandey R. et al., Indian J.Biochem. Biophys. 2007, 44, 295.4. The anticancer agent prodigiosin induces p21WAF1/CIP1 expression via transforming growth factorbetareceptor pathway. Soto-Cerrato V. et al., Biochem Pharmacol. 2007, 74, 1340.5. Prodigiosin induces the proapoptotic gene NAG-1 via glycogen synthase kinase-3beta activity in humanbreast cancer cells. Soto-Cerrato V. et al., Mol. Cancer Ther. 2007, 6, 362.6. Proteomic analysis of prodigiosin-induced apoptosis in a breast cancer mitoxantrone-resistant (MCF-7MR) cell line. Monge M. et al., Invest. New Drugs 2007, 25, 21.

优质前列腺素E2Mouse PGE2,PG-E2 ELISA试剂盒有国外文献支持 特异性：本试剂盒可同时检测天然或重组的，且与其他相关蛋白无交叉反应。有效期：6个月预期应用：ELISA法定量测定血清、血浆、细胞培养上清或其它相关生物液体中的含量。说明1．试剂盒保存：-20℃（较长时间不用时）；2-8℃（频繁使用时）。2．浓洗涤液低温保存会有盐析出，稀释时可在水浴中加温助溶。3．中、英文说明书可能会有不一致之处，请以英文说明书为准。4．刚开启的酶联板孔中可能会含有少许水样物质，此为正常现象，不会对实验结果造成任何影响。实验原理用纯化的抗体包被微孔板，制成固相载体，往包被抗该指标抗体的微孔中依次加入标本或标准品、生物素化的抗该指标抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的该指标呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。试剂盒组成及试剂配制1.         酶联板（Assay plate ）：一块（96孔）。2.         标准品（Standard）：2瓶（冻干品）。3.         样品稀释液（Sample Diluent）：1×20ml/瓶。4.         生物素标记抗体稀释液（Biotin-antibody Diluent）：1×10ml/瓶。5.         辣根过氧化物酶标记亲和素稀释液 （HRP-avidin Diluent）：1×10ml/瓶。6.         生物素标记抗体（Biotin-antibody）：1×120μl/瓶（1：100）7.         辣根过氧化物酶标记亲和素（HRP-avidin）：1×120μl/瓶（1：100）8.         底物溶液（TMB Substrate）：1×10ml/瓶。9.         浓洗涤液（Wash Buffer）：1×20ml/瓶，使用时每瓶用蒸馏水稀释25倍。10.     终止液（Stop Solution）：1×10ml/瓶（2N H2SO4）。 需要而未提供的试剂和器材1.         标准规格酶标仪2.         高速离心机3.         电热恒温培养箱4.         干净的试管和Eppendof管5.         系列可调节移液器及吸头，一次检测样品较多时，最好用多通道移液器6.    蒸馏水，容量瓶等标本的采集及保存1.         血清：全血标本请于室温放置2小时或4℃过一晚后于1000 x g离心20分钟，取上清即可检测，或将标本放于-20℃或-80℃保存，但应避免反复冻融。2.         血浆：可用EDTA或肝素作为抗凝剂，标本采集后30分钟内于2 - 8° C 1000 x g离心15分钟，或将标本放于-20℃或-80℃保存，但应避免反复冻融。3.         细胞培养物上清或其它生物标本：1000 x g离心20分钟，取上清即可检测，或将标本放于-20℃或-80℃保存，但应避免反复冻融。注：标本溶血会影响最后检测结果，因此溶血标本不宜进行此项检测。标本的稀释原则：首先通过文献检索的方式了解待测样本的大致含量，确定适当的稀释倍数。只有稀释至标准曲线的范围内，检测的结果才是准确的。稀释的过程中，应做好详细的记录。最后计算浓度时，稀释了“N”倍，标本的浓度应再乘以“N”。标准品的稀释原则：2瓶，每瓶临用前以样品稀释液稀释至1ml，盖好后静置10分钟以上，然后反复颠倒/搓动以助溶解，其浓度为300 pg/ml，做系列倍比稀释后，分别稀释300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，样品稀释液直接作为标准浓度0 pg/ml，临用前15分钟内配制。如配制150 pg/ml标准品：取0.5ml（不要少于0.5ml）300 pg/ml的上述标准品加入含0.5ml样品稀释液的Eppendorf管中，混匀即可，其余浓度以此类推。生物素标记抗体的稀释原则：临用前以生物素标记抗体稀释液稀释，稀释前根据预先计算好的每次实验所需的总量配制（每孔100μl），实际配制时应多配制0.1-0.2ml。如10μl生物素标记抗体加990μl生物素标记抗体稀释液的比例配制，轻轻混匀，在使用前一小时内配制。辣根过氧化物酶标记亲和素的稀释原则：临用前以辣根过氧化物酶标记亲和素稀释液稀释，稀释前根据预先计算好的每次实验所需的总量配制（每孔100μl），实际配制时应多配制0.1-0.2ml。如10μl辣根过氧化物酶标记亲和素加990μl辣根过氧化物酶标记亲和素稀释液 的比例配制，轻轻混匀，在使用前一小时内配制。操作步骤实验开始前，请提前配置好所有试剂，试剂或样品稀释时，均需混匀，混匀时尽量避免起泡。每次检测都应该做标准曲线。如样品浓度过高时，用样品稀释液进行稀释，以使样品符合试剂盒的检测范围。1.         加样：分别设空白孔、标准孔、待测样品孔。空白孔加样品稀释液100μl，余孔分别加标准品或待测样品100μl，注意不要有气泡，加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀，酶标板加上盖或覆膜，37℃反应120分钟。为保证实验结果有效性，每次实验请使用新的标准品溶液。2.         弃去液体，甩干，不用洗涤。每孔加生物素标记抗体工作液 100μl（取1μl生物素标记抗体加99μl生物素标记抗体稀释液的比例配制，轻轻混匀，在使用前一小时内配制），37℃,60分钟。3.         温育60分钟后，弃去孔内液体，甩干，洗板3次，每次浸泡1-2分钟，350μl/每孔，甩干。4.         每孔加辣根过氧化物酶标记亲和素工作液（同生物素标记抗体工作液） 100μl，37℃，60分钟。5.         温育60分钟后，弃去孔内液体，甩干，洗板5次，每次浸泡1-2分钟，350μl/每孔，甩干。6.         依序每孔加底物溶液90μl，37℃避光显色（30分钟内，此时肉眼可见标准品的前3-4孔有明显的梯度蓝色，后3-4孔梯度不明显，即可终止）。7.         依序每孔加终止溶液50μl，终止反应（此时蓝色立转黄色）。终止液的加入顺序应尽量与底物液的加入顺序相同。为了保证实验结果的准确性，底物反应时间到后应尽快加入终止液。8.         用酶联仪在450nm波长依序测量各孔的光密度（OD值）。 在加终止液后15分钟以内进行检测。注：1. 用户在初次使用试剂盒时，应将各种试剂管离心数分钟，以便试剂集中到管底。2. 每次实验留一孔作为空白调零孔，该孔不加任何试剂，只是最后加底物溶液及2N H2SO4。测量时先用此孔调OD值至零。3. 为防止样品蒸发，试验时将反应板放于铺有湿布的密闭盒内，酶标板加上盖或覆膜。4. 未使用完的酶标板或者试剂，请于2-8℃保存。标准品、生物素标记抗体工作液、辣根过氧化物酶标记亲和素工作液请依据所需的量配置使用。请勿重复使用已稀释过的标准品、生物素标记抗体工作液或、辣根过氧化物酶标记亲和素工作液。5. 建议检测样品时均设双孔测定，以保证检测结果的准确性。洗板方法 手工洗板方法：吸去（不可触及板壁）或甩掉酶标板内的液体；在实验台上铺垫几层吸水纸，酶标板朝下用力拍几次；将推荐的洗涤缓冲液至少0.3ml注入孔内，浸泡1-2分钟。根据需要，重复此过程数次。 自动洗板：如果有自动洗板机，应在熟练使用后再用到正式实验过程中。计算以标准物的浓度为横坐标（对数坐标），OD值为纵坐标（普通坐标），在半对数坐标纸上绘出标准曲线，根据样品的OD值由标准曲线查出相应的浓度；再乘以稀释倍数；或用标准物的浓度与OD值计算出标准曲线的直线回归方程式，将样品的OD值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实际浓度。注意事项1. 当混合蛋白溶液时应尽量轻缓，避免起泡。2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。3. 一次加样时间最好控制在5分钟内，如标本数量多，推荐使用排枪加样。4. 请每次测定的同时做标准曲线，最好做复孔。5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请最后乘以稀释倍数。6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。7. 底物请避光保存。8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

进口操作说明重组二甲基甘氨酸氧化酶/DMGOSynonymsDMGO, Dimethylglycine Oxidase.IntroductionDimethylglycine oxidase (DMGO) is a covalent flavoenzyme from Arthrobacter globiformis that catalyzes the oxidative demethylation of dimethylglycine to yield sarcosine, formaldehyde, and hydrogen peroxide. The N-terminal region binds FAD covalently so it is yellowish.DescriptionDimethylglycine oxidase Recombinant originated from Arthrobacter globifomis fused to His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 850 amino acids and having a molecular mass of 92.1 kDa. The DMGO is purified by proprietary chromatographic techniques.SourceEscherichia Coli.Physical AppearanceSterile filtered liquid formulation 1 mg/ml.FormulationThe Recombinant Dimethylglycine Oxidase solution contains 20mM Tris-HCl pH7.5 and 20% glycerol.StabilityDimethylglycine Oxidase Recombinant although stable at 4°C for 30 days, should be stored desiccated below -20°C for periods greater than 30 days. Please prevent freeze-thaw cycles.PurityGreater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.Amino acid sequenceMGSSHHHHHH SSGLVPRGSH MASTPRIVII GAGIVGTNLA DELVTRGWNN ITVLDQGPLN MPGGSTSHAP GLVFQTNPSK TMASFAKYTVEKLLSLTEDG VSCFNQVGGL EVATTETRLA DLKRKLGYAA AWGIEGRLLS PAECQELYPL LDGENILGGL HVPSDGLASA ARAVQLLIKRTESAGVTYRG STTVTGIEQS GGRVTGVQTA DGVIPADIVV SCAGFWGAKI GAMIGMAVPL LPLAHQYVKT TPVPAQQGRN DQPNGARLPILRHQDQDLYY REHGDRYGIG SYAHRPMPVD VDTLGAYAPE TVSEHHMPSR LDFTLEDFLP AWEATKQLLP ALADSEIEDG FNGIFSFTPDGGPLLGESKE LDGFYVAEAV WVTHSAGVAK AMAELLTTGR SETDLGECDI TRFEDVQLTP EYVSETSQQN FVEIYDVLHP LQPRLSPRNLRVSPFHARHK ELGAFFLEAG GWERPYWFEA NAALLKEMPA EWLPPARDAW SGMFSSPIAA AEAWKTRTAV AMYDMTPLKR LEVSGPGALKLLQELTTADL AKKPGAVTYT LLLDHAGGVR SDITVARLSE DTFQLGANGN IDTAYFERAA RHQTQSGSAT DWVQVRDTTG GTCCIGLWGPLARDLVSKVS DDDFTNDGLK YFRAKNVVIG GIPVTAMRLS YVGELGWELY TSADNGQRLW DALWQAGQPF GVIAAGRAAF SSLRLEKGYRSWGTDMTTEH DPFEAGLGFA VKMAKESFIG KGALEGRTEE ASARRLRCLT IDDGRSIVLG KEPVFYKEQA VGYVTSAAYG YTVAKPIAYSYLPGTVSVGD SVDIEYFGRR ITATVTEDPL YDPKMTRLRG.UsageProspecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

以色列进口小鼠白介素-6/IL 6 Mouse  重组小鼠白介素-6/IL 6 MouseSynonymsIFN-b2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B-cell hybridoma growth factor.IntroductionInterleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.DescriptionInterleukin-6 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21709 Dalton. The IL-6 is purified by proprietary chromatographic techniques.SourceEscherichia Coli.Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.FormulationLyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.SolubilityIt is recommended to reconstitute the lyophilized Mouse Il-6 in sterile 18M?-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.StabilityLyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.PurityGreater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.Amino acid sequenceFPTSQVRRGD FTEDTTPNRP VYTTSQVGGL ITHVLWEIVE MRKELCNGNS DCMNNDDALA ENNLKLPEIQ RNDGCYQTGY NQEICLLKIS SGLLEYHSYL EYMKNNLKDN KKDKARVLQR DTETLIHIFN QEVKDLHKIV LPTPISNALL TDKLESQKEW LRTKTIQFIL KSLEEFLKVT LRSTRQT.Biological ActivityThe ED50 as determined by the dose-dependant stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is 50,000,000 units/mg.ReferencesTitle:Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice.Publication:Published online before print March 30, 2010, doi: 10.1096/fj.09-148155 August 2010 The FASEB Journal vol. 24 no. 8 2869-2880 .Link:http://www.fasebj.org/content/24/8/2869.fullUsageProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

又便宜又质量有保证的小鼠粒-巨噬细胞集落刺激因子/GM CSF Mouse 重组小鼠粒-巨噬细胞集落刺激因子/GM CSF MouseSynonymsCSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim.IntroductionGMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.DescriptionGranulocyte Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14285.35 Dalton.GM-CSF Mouse is purified by proprietary chromatographic techniques.SourceEscherichia Coli.Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.FormulationGM-CSF Mouse was lyophilized with no additives.SolubilityIt is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 20mM AcOH (acetic Acid) not less than 100μg/ml, which can then be further diluted to other aqueous solutions.StabilityLyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GM-CSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.PurityGreater than 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.Amino acid sequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.Biological ActivityThe ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is 1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard.References1.Title:Multigene/multisubtype HIV-1 vaccine induces potent cellular and humoral immune responses by needle-free intradermal delivery.Publication: Mol Ther. 2005 Dec;12(6):1197-205. Epub 2005 Aug 22. Pmid: 16112909Link: http://www.nature.com/mt/journal/v12/n6/full/mt20051405a.htmlApplications: The Mouse GM-CSF used for 2 purposes: 1. As an adjuvant for DNA vaccine which included seven plasmids encoding nine HIV-1 proteins. The mice were injected with the DNA vaccine together with recombinant mouse GM-CSF. 2. Used in Elisa.2.Title: Neospora caninum: cloning and expression of a gene coding for cytokine-inducing profilin. Publication: Exp Parasitol. 2010 Aug;125(4):357-62. Epub 2010 Mar 6. Pmid: 20211619Link: http://www.sciencedirect.com/science/article/pii/S001448941000086XApplications: Used for immunoblotting3.Title: Intranasal Granulocyte-Macrophage Colony-Stimulating Factor Reduces the AspergillusBurden in an Immunosuppressed Murine Model of Pulmonary Aspergillosis.Publication: Antimicrob Agents Chemother. 2008 February; 52(2): 716–718. Published online 2007 November 5Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224773/?tool=pmcentrezApplications: GM-CSF as tested a therapeutic potential in a murine model of pulmonary aspergillosis. In summary, this pilot study indicates that GM-CSF administered intranasally may be a novel therapeutic approach for the prevention or treatment of pulmonary fungal infections and may augment the efficacies of antifungal agents.GM-CSF was given intranasal.4.Title: Dendritic Cell-Based Therapeutic Vaccination against Myeloma: Vaccine Formulation Determines Efficacy against Light Chain Myeloma Publication: The Journal of Immunology February 1, 2009 vol. 182 no. 3 1667-1673 Link: http://www.jimmunol.org/content/182/3/1667.full5.Title:Pre-clinical Evaluation of a CEA DNA Prime/protein Boost Vaccination Strategy Against Colorectal Cancer.Publication:Article first published online: 21 JUN 2007 DOI:10.1111/j.1365-3083.2007.01945.xScandinavian Journal of Immunology Volume 66, Issue 1, pages 43–51, July 2007Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3083.2007.01945.x/full6.Title:Intranasal Granulocyte-Macrophage Colony-Stimulating Factor Reduces the AspergillusBurden in an Immunosuppressed Murine Model of Pulmonary Aspergillosis.Publication: First published November 2007, doi: 10.1128/?AAC.00760-07 Antimicrob. Agents Chemother. February 2008 vol. 52 no. 2 716-718 Link:http://aac.asm.org/content/52/2/716.full UsageProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

PTX3 Human/重组人正五聚蛋白3 PTX3SynonymsTSG-14, TNFAIP5, PTX3, Pentraxin-related protein PTX3, Pentaxin-related protein PTX3, Tumor necrosis factor-inducible gene 14 protein, TSG14, pentraxin-related gene rapidly induced by IL-1 beta.IntroductionPTX3 is part of the pentraxin family sharing the C-terminal domain with short pentraxins and containing a unique N-terminal domain. PTX3 is produced and released at inflammatory sites by various cell types including monocytes/macrophages, endothelial cells, vascular smooth muscle cells, fibroblasts, and adipocytes. PTX3 is involved in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. PTX3 is used as a marker for disease activity of psoriasis. High serum PTX3 levels are associated with the disease severity of systemic sclerosis. Elevated serum PTX3 is associated with pulmonary fungal infections.DescriptionRecombinant Human PTX3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 401 amino acids (18-381 a.a) and having a molecular mass of 44.4 kDa. PTX3 is fused to a 36 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.SourceEscherichia Coli.Physical AppearanceSterile filtered colorless solution.FormulationThe PTX3 protein contains 20mM Tris-HCl buffer pH-8.5, 1mM DTT and 10% glycerol.StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.PurityGreater than 90% as determined by Analysis by SDS-PAGE.SequenceMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMENS DDYDLMYVNL DNEIDNGLHP TEDPTPCDCG QEHSEWDKLF IMLENSQMRE RMLLQATDDV LRGELQRLRE ELGRLAESLA RPCAPGAPAE ARLTSALDEL LQATRDAGRR LARMEGAEAQ RPEEAGRALA AVLEELRQTR ADLHAVQGWA ARSWLPAGCE TAILFPMRSK KIFGSVHPVR PMRLESFSAC IWVKATDVLN KTILFSYGTK RNPYEIQLYL SYQSIVFVVG GEENKLVAEA MVSLGRWTHL CGTWNSEEGL TSLWVNGELA ATTVEMATGH IVPEGGILQI GQEKNGCCVG GGFDETLAFS GRLTGFNIWD SVLSNEEIRE TGGAESCHIR GNIVGWGVTE IQPHGGAQYV S.UsageProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

human alox5 full-length orf ( np_000689.1, 1 a.a. - 674 a.a.) alox5 (human) recombinant protein (p01)product description:human alox5 full-length orf ( np_000689.1, 1 a.a. - 674 a.a.) recombinant protein with gst-tag at n-terminal.sequence:mpsytvtvatgsqwfagtddyiylslvgsagcsekhlldkpfyndfergavdsydvtvdeelgeiqlvriekrkywlnddwylkyitlktphgdyiefpcyrwitgdvevvlrdgraklarddqihilkqhrrkeletrqkqyrwmewnpgfplsidakchkdlprdiqfdsekgvdfvlnyskamenlfinrfmhmfqsswndfadfekifvkisntiservmnhwqedlmfgyqflngcnpvlirrctelpeklpvttemvecslerqlsleqevqqgnifivdfelldgidanktdpctlqflaapicllyknlankivpiaiqlnqipgdenpiflpsdakydwllakiwvrssdfhvhqtithllrthlvsevfgiamyrqlpavhpifkllvahvrftiaintkareqlicecglfdkanatgggghvqmvqramkdltyaslcfpeaikargmeskedipyyfyrddgllvweairtftaevvdiyyegdqvveedpelqdfvndvyvygmrgrkssgfpksvksreqlseyltvviftasaqhaavnfgqydwcswipnapptmrappptakgvvtieqivdtlpdrgrscwhlgavwalsqfqenelflgmypeehfiekpvkeamarfrknleaivsviaernkkkqlpyyylspdripnsvaihost:wheat germ (in vitro)theoretical mw (kda):104.4preparation method:in vitro wheat germ expression systempurification:glutathione sepharose 4 fast flowquality control testing:12.5% sds-page stained with coomassie blue. storage buffer:50 mm tris-hci, 10 mm reduced glutathione, ph=8.0 in the elution buffer.storage instruction:store at -80°c. aliquot to avoid repeated freezing and thawing.note:best use within three months from the date of receipt of this protein.

human alox5 full-length orf ( np_000689.1, 1 a.a. - 674 a.a.) alox5 (human) recombinant protein (p01)product description:human alox5 full-length orf ( np_000689.1, 1 a.a. - 674 a.a.) recombinant protein with gst-tag at n-terminal.sequence:mpsytvtvatgsqwfagtddyiylslvgsagcsekhlldkpfyndfergavdsydvtvdeelgeiqlvriekrkywlnddwylkyitlktphgdyiefpcyrwitgdvevvlrdgraklarddqihilkqhrrkeletrqkqyrwmewnpgfplsidakchkdlprdiqfdsekgvdfvlnyskamenlfinrfmhmfqsswndfadfekifvkisntiservmnhwqedlmfgyqflngcnpvlirrctelpeklpvttemvecslerqlsleqevqqgnifivdfelldgidanktdpctlqflaapicllyknlankivpiaiqlnqipgdenpiflpsdakydwllakiwvrssdfhvhqtithllrthlvsevfgiamyrqlpavhpifkllvahvrftiaintkareqlicecglfdkanatgggghvqmvqramkdltyaslcfpeaikargmeskedipyyfyrddgllvweairtftaevvdiyyegdqvveedpelqdfvndvyvygmrgrkssgfpksvksreqlseyltvviftasaqhaavnfgqydwcswipnapptmrappptakgvvtieqivdtlpdrgrscwhlgavwalsqfqenelflgmypeehfiekpvkeamarfrknleaivsviaernkkkqlpyyylspdripnsvaihost:wheat germ (in vitro)theoretical mw (kda):104.4preparation method:in vitro wheat germ expression systempurification:glutathione sepharose 4 fast flowquality control testing:12.5% sds-page stained with coomassie blue. storage buffer:50 mm tris-hci, 10 mm reduced glutathione, ph=8.0 in the elution buffer.storage instruction:store at -80°c. aliquot to avoid repeated freezing and thawing.note:best use within three months from the date of receipt of this protein.

人1,25二羟基维生素D3(DVD/DHVD3)ELISA kit产品类型:ELISA Kit产品名称:人1,25二羟基维生素D3(DVD/DHVD3)ELISA kit英文名称:Human 1,25-dihydroxyvitamin D3 (DVD/DHVD3) ELISA kit货号:CSB-E05120h规格:96T中文价格:5500种属:Human待测物名称:1,25-dihydroxyvitamin D3缩写:DVD/DHVD3检测范围:1000 fmol/L-5000 fmol/L灵敏度:250 fmol/L反应时间:1-5h所需样本体积:50-100ul检测波长:450 nm

Recombinant Human Metallothionein-3(MT3)产品类型:Recombinant Protein产品名称:Recombinant Human Metallothionein-3(MT3)货号:CSB-YP015122HU   >>   YeastCSB-EP015122HU   >>   E.coliCSB-BP015122HU   >>   BaculovirusCSB-MP015122HU   >>   Mammalian cell规格:1mg蛋白名称:Recommended name: Metallothionein-3 Short name= MT-3 Alternative name(s): GIFB Short name= GIF Growth inhibitory factor Metallothionein-III Short name= MT-III―基因名称:Name:MT3种属:Homo sapiens (Human)蛋白标签:His tagged蛋白纯度:>90% (SDS-PAGE)缓冲液:Tris-based buffer，50% glycerol蛋白储存:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. ―使用注意:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.说明书:氨基酸序列:MDPETCPCPS GGSCTCADSC KCEGCKCTSC KKSCCSCCPA ECEKCAKDCV CKGGEAAEAEAEKCSCCQ表达区域:1-68

重组溶葡萄球菌素/LysostaphinSynonymsLysostaphin, EC 3.4.24.75, Glycyl-glycine endopeptidase.DescriptionLysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination.The Mw of lysostaphin is 26,921 (Recsei et al, PNAS 1987).SourceEscherichia Coli.Physical AppearanceSterile Filtered lyophilized powder.FormulationThe protein was lyophilized without any additives.StabilityLysostaphin although stable at 4°C for 6 months, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.Lysostaphin has optimal stability in the range of pH 4.5, and optimal activity in the range of pH 8. For a stock solution it is recommended to work with 10mg/ml lysostaphin in 10mM sodium acetate pH 4.5.SolubilityIt is recommended to reconstitute the lyophilized Lysostaphin in 20mM sodium acetate, pH 4.5, which can then be further diluted to other aqueous solutions.Purity96.9% as determined by RP-HPLC.Biological ActivityDetermined by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH8.0, 30°C.For a reaction buffer it is recommended to work with 200mM Tris-HCl pH 8.Specific ActivityDetermined to be 4,243 units/mg.Protein contentProtein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 2.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis RP-HPLC, using a calibrated solution of Lysostaphin as a Reference Standard.UsageProspec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

重组人基质金属蛋白酶2/MMP-2 HumanSynonyms72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, TBE-1, MMP2, CLG4A, CLG4, MONA, MMP-II.IntroductionMatrix metalloproteinase-2 (MMP-2) is a type IV collagenase, which is involved in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. MMP-2 contains a number of distinct domains: a prodomain that is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin like domain believed to have a role in substrate targeting; and a carboxyl terminal (hemopexin like) domain containing 2 N-linked glycosylation. The MMP-2 can degrade an extensive array of substrates including type IV, V, VII and X collagens as well as gelatin type I. In addition, MMP-2 interacts with THBS2, TIMP2, Thrombospondin 1, CCL7 and TIMP4. MMP-2 autocatalytic cleavage in the C-terminal generates the anti-angiogenic peptide, PEX. This process seems to be made possible by binding integrinv/beta3. Defects in the MMP-2 are the cause of Torg-Winchester syndrome (TWS), aka multicentric osteolysis nodulosis and arthropathy (MONA).DescriptionMMP-2 Human Recombinant produced in HEK293 cells is a proform of the Human MMP-2 (Ala30-Cys660) and fused with a ployhistide tag at the C-terminus, having an Mw of 71kDa. MMP-2 is purified by proprietary chromatographic techniques.SourceHEK293 cells.Physical AppearanceThe MMP-2 is supplied as a sterile Filtered colorless solution.FormulationThe MMP-2 is supplied as a 0.2μm filtered solution in 20mM Tris-HCl, 150mM NaCl and 0.05% Brij 35, pH 7.4.StabilityStore MMP-2 at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles.PurityGreater than 95% as determined by SDS-PAGE.Biological ActivityThe activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RND,Catalog # ES001)., The specific activity is > 1,000 pmoles/min/μg.Recombinant Human MMP-2 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).Activation Protocol:1. Dilute MMP2 to 100μg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP2 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.3. Incubate at 37°C for 1 hour.ReferencesTitle:MMP-2 regulates human platelet activation by interacting withintegrin aIIbb 3.Publication:To cite this article: Choi W-S, Jeon O-H, Kim H-H, Kim D-S. MMP-2 regulates human platelet activation by interacting with integrin aIIbb3.J Thromb Haemost 2008; 6: 517–23.Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1538-7836.2007.02871.x/pdfUsageProspec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

大鼠5羟色胺(5-ht)elisa 说明书大鼠5羟色胺(5-ht)酶联免疫分析试剂盒使用说明书本试剂仅供研究使用       目的：本试剂盒用于测定大鼠血清，血浆及相关液体样本中5羟色胺(5-ht)的含量。实验原理：   本试剂盒应用双抗体夹心法测定标本中大鼠5羟色胺(5-ht)水平。用纯化的大鼠5羟色胺(5-ht)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入5羟色胺(5-ht)，再与hrp标记的5羟色胺(5-ht)抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物tmb显色。tmb在hrp酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的5羟色胺(5-ht)呈正相关。用酶标仪在450nm波长下测定吸光度（od值），通过标准曲线计算样品中大鼠5羟色胺(5-ht)浓度。 试剂盒组成：试剂盒组成48孔配置96孔配置保存说明书1份1份封板膜2片（48）2片（96）密封袋1个1个酶标包被板1×481×962-8℃保存标准品：540ng/l0.5ml×1瓶0.5ml×1瓶2-8℃保存标准品稀释液1.5ml×1瓶1.5ml×1瓶2-8℃保存酶标试剂3 ml×1瓶6 ml×1瓶2-8℃保存样品稀释液3 ml×1瓶6 ml×1瓶2-8℃保存显色剂a液3 ml×1瓶6 ml×1瓶2-8℃保存显色剂b液3 ml×1瓶6 ml×1瓶2-8℃保存终止液3ml×1瓶6ml×1瓶2-8℃保存浓缩洗涤液（20ml×20倍）×1瓶（20ml×30倍）×1瓶2-8℃保存 样本处理及要求：1. 血清：室温血液自然凝固10-20分钟，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如出现沉淀，应再次离心。2. 血浆：应根据标本的要求选择edta或柠檬酸钠作为抗凝剂，混合10-20分钟后，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如有沉淀形成，应该再次离心。3. 尿液：用无菌管收集，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如有沉淀形成，应再次离心。胸腹水、脑脊液参照实行。4. 细胞培养上清：检测分泌性的成份时，用无菌管收集。离心20分钟左右（2000-3000转/分）。仔细收集上清。检测细胞内的成份时，用pbs（ph7.2-7.4）稀释细胞悬液，细胞浓度达到100万/ml左右。通过反复冻融，以使细胞破坏并放出细胞内成份。离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。5. 组织标本：切割标本后，称取重量。加入一定量的pbs，ph7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的pbs（ph7.4），用手工或匀浆器将标本匀浆充分。离心20分钟左右（2000-3000转/分）。仔细收集上清。分装后一份待检测，其余冷冻备用。6. 标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融.7. 不能检测含nan3的样品，因nan3抑制辣根过氧化物酶的（hrp）活性。 操作步骤：1. 标准品的稀释与加样：在酶标包被板上设标准品孔10孔，在第一、第二孔中分别加标准品100μl，然后在第一、第二孔中加标准品稀释液50μl，混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液50μl，混匀；然后在第三孔和第四孔中先各取50μl弃掉，再各取50μl分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液50ul，混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液50μl，混匀后从第七、第八孔中分别取50μl加到第九、第十孔中，再在第九第十孔分别加标准品稀释液50μl，混匀后从第九第十孔中各取50μl弃掉。（稀释后各孔加样量都为50μl，浓度分别为360 ng/l,240ng/l ,120 ng/l,60ng/l,30 ng/l）。2. 加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40μl，然后再加待测样品10μl（样品最终稀释度为5倍）。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。3. 温育：用封板膜封板后置37℃温育30分钟。4. 配液：将30（48t的20倍）倍浓缩洗涤液用蒸馏水30（48t的20倍）倍稀释后备用。5. 洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30秒后弃去，如此重复5次，拍干。6. 加酶：每孔加入酶标试剂50μl，空白孔除外。7. 温育：操作同3。8. 洗涤：操作同5。9. 显色：每孔先加入显色剂a50μl，再加入显色剂b50μl，轻轻震荡混匀，37℃避光显色15分钟. 10. 终止：每孔加终止液50μl，终止反应（此时蓝色立转黄色）。11. 测定：以空白空调零，450nm波长依序测量各孔的吸光度（od值）。 测定应在加终止液后15分钟以内进行。 注意事项：1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。2． 浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。3． 各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。一次加样时间最好控制在5分钟内，如标本数量多，推荐使用排枪加样。4． 请每次测定的同时做标准曲线，最好做复孔。如标本中待测物质含量过高（样本od值大于标准品孔第一孔的od值），请先用样品稀释液稀释一定倍数（n倍）后再测定，计算时请最后乘以总稀释倍数（×n×5）。5． 封板膜只限一次性使用，以避免交叉污染。6． 底物请避光保存。7． 严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准.8． 所有样品，洗涤液和各种废弃物都应按传染物处理。9． 本试剂不同批号组分不得混用。10. 如与英文说明书有异，以英文说明书为准。  计算：以标准物的浓度为横坐标，od值为纵坐标，    在坐标纸上绘出标准曲线，根据样品的od      值由标准曲线查出相应的浓度；再乘以稀释      倍数；或用标准物的浓度与od值计算出标      准曲线的直线回归方程式，将样品的od值      代入方程式，计算出样品浓度，再乘以稀释      倍数，即为样品的实际浓度。                                                                   （此图仅供参考）   试剂盒性能：1.样品线性回归与预期浓度相关系数r值为0.990以上。2.批内与批间应分别小于9%和11%  检测范围：                                              20ng/l -400ng/l                                                                   保存条件及有效期：1.试剂盒保存：；2-8℃。2．有效期：6个月       

大鼠血管舒缓激肽（bk）elisa说明书大鼠血管舒缓激肽（bk）酶联免疫分析试剂盒使用说明书本试剂仅供研究使用       目的：本试剂盒用于测定大鼠血清，血浆，细胞上清及相关液体样本中血管舒缓激肽（bk）的含量。实验原理：   本试剂盒应用双抗体夹心法测定标本中大鼠血管舒缓激肽（bk）水平。用纯化的大鼠血管舒缓激肽（bk）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入血管舒缓激肽（bk），再与hrp标记的血管舒缓激肽（bk）抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物tmb显色。tmb在hrp酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管舒缓激肽（bk）呈正相关。用酶标仪在450nm波长下测定吸光度（od值），通过标准曲线计算样品中大鼠血管舒缓激肽（bk）浓度。 试剂盒组成：试剂盒组成48孔配置96孔配置保存说明书1份1份封板膜2片（48）2片（96）密封袋1个1个酶标包被板1×481×962-8℃保存标准品：90μg/ml0.5ml×1瓶0.5ml×1瓶2-8℃保存标准品稀释液1.5ml×1瓶1.5ml×1瓶2-8℃保存酶标试剂3 ml×1瓶6 ml×1瓶2-8℃保存样品稀释液3 ml×1瓶6 ml×1瓶2-8℃保存显色剂a液3 ml×1瓶6 ml×1瓶2-8℃保存显色剂b液3 ml×1瓶6 ml×1瓶2-8℃保存终止液3ml×1瓶6ml×1瓶2-8℃保存浓缩洗涤液（20ml×20倍）×1瓶（20ml×30倍）×1瓶2-8℃保存 样本处理及要求：1. 血清：室温血液自然凝固10-20分钟，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如出现沉淀，应再次离心。2. 血浆：应根据标本的要求选择edta或柠檬酸钠作为抗凝剂，混合10-20分钟后，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如有沉淀形成，应该再次离心。3. 尿液：用无菌管收集，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如有沉淀形成，应再次离心。胸腹水、脑脊液参照实行。4. 细胞培养上清：检测分泌性的成份时，用无菌管收集。离心20分钟左右（2000-3000转/分）。仔细收集上清。检测细胞内的成份时，用pbs（ph7.2-7.4）稀释细胞悬液，细胞浓度达到100万/ml左右。通过反复冻融，以使细胞破坏并放出细胞内成份。离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。5. 组织标本：切割标本后，称取重量。加入一定量的pbs，ph7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的pbs（ph7.4），用手工或匀浆器将标本匀浆充分。离心20分钟左右（2000-3000转/分）。仔细收集上清。分装后一份待检测，其余冷冻备用。6. 标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融.7. 不能检测含nan3的样品，因nan3抑制辣根过氧化物酶的（hrp）活性。 操作步骤：1. 标准品的稀释与加样：在酶标包被板上设标准品孔10孔，在第一、第二孔中分别加标准品100μl，然后在第一、第二孔中加标准品稀释液50μl，混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液50μl，混匀；然后在第三孔和第四孔中先各取50μl弃掉，再各取50μl分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液50ul，混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液50μl，混匀后从第七、第八孔中分别取50μl加到第九、第十孔中，再在第九第十孔分别加标准品稀释液50μl，混匀后从第九第十孔中各取50μl弃掉。（稀释后各孔加样量都为50μl，浓度分别为60μg/ml，40μg/ml ，20μg/ml，10μg/ml，5μg/ml）。2. 加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40μl，然后再加待测样品10μl（样品最终稀释度为5倍）。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。3. 温育：用封板膜封板后置37℃温育30分钟。4. 配液：将30（48t的20倍）倍浓缩洗涤液用蒸馏水30（48t的20倍）倍稀释后备用。5. 洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30秒后弃去，如此重复5次，拍干。6. 加酶：每孔加入酶标试剂50μl，空白孔除外。7. 温育：操作同3。8. 洗涤：操作同5。9. 显色：每孔先加入显色剂a50μl，再加入显色剂b50μl，轻轻震荡混匀，37℃避光显色15分钟. 10. 终止：每孔加终止液50μl，终止反应（此时蓝色立转黄色）。11. 测定：以空白空调零，450nm波长依序测量各孔的吸光度（od值）。 测定应在加终止液后15分钟以内进行。 注意事项：1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。2． 浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。3． 各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。一次加样时间最好控制在5分钟内，如标本数量多，推荐使用排枪加样。4． 请每次测定的同时做标准曲线，最好做复孔。如标本中待测物质含量过高（样本od值大于标准品孔第一孔的od值），请先用样品稀释液稀释一定倍数（n倍）后再测定，计算时请最后乘以总稀释倍数（×n×5）。5． 封板膜只限一次性使用，以避免交叉污染。6． 底物请避光保存。7． 严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准.8． 所有样品，洗涤液和各种废弃物都应按传染物处理。9． 本试剂不同批号组分不得混用。10. 如与英文说明书有异，以英文说明书为准。  计算：以标准物的浓度为横坐标，od值为纵坐标，    在坐标纸上绘出标准曲线，根据样品的od      值由标准曲线查出相应的浓度；再乘以稀释      倍数；或用标准物的浓度与od值计算出标      准曲线的直线回归方程式，将样品的od值      代入方程式，计算出样品浓度，再乘以稀释      倍数，即为样品的实际浓度。                                                                   （此图仅供参考）   试剂盒性能：1.样品线性回归与预期浓度相关系数r值为0.990以上。2.批内与批见应分别小于9%和11%  检测范围：                                              2μg/ml -70 μg/ml                                                                    保存条件及有效期：1.试剂盒保存：；2-8℃。2．有效期：6个月       

人转化生长因子β1（tgf-β1）说明书人转化生长因子β1（tgf-β1）酶联免疫分析试剂盒使用说明书本试剂仅供研究使用       目的：本试剂盒用于测定人血清，血浆及相关液体样本中转化生长因子β1（tgf-β1）的含量。实验原理：本试剂盒应用双抗体夹心法测定标本中人转化生长因子β1（tgf-β1）水平。用纯化的人转化生长因子β1（tgf-β1）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中加入转化生长因子β1（tgf-β1），再与hrp标记的转化生长因子β1（tgf-β1）抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物tmb显色。tmb在hrp酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的转化生长因子β1（tgf-β1）呈正相关。用酶标仪在450nm波长下测定吸光度（od值），通过标准曲线计算样品中人转化生长因子β1（tgf-β1）的含量。 试剂盒组成：试剂盒组成48孔配置96孔配置保存说明书1份1份封板膜2片（48）2片（96）密封袋1个1个酶标包被板1×481×962-8℃保存标准品：1800ng/l0.5ml×1瓶0.5ml×1瓶2-8℃保存标准品稀释液1.5ml×1瓶1.5ml×1瓶2-8℃保存酶标试剂3 ml×1瓶6 ml×1瓶2-8℃保存样品稀释液3 ml×1瓶6 ml×1瓶2-8℃保存显色剂a液3 ml×1瓶6 ml×1瓶2-8℃保存显色剂b液3 ml×1瓶6 ml×1瓶2-8℃保存终止液3ml×1瓶6ml×1瓶2-8℃保存浓缩洗涤液（20ml×20倍）×1瓶（20ml×30倍）×1瓶2-8℃保存 样本处理及要求：1. 血清：室温血液自然凝固10-20分钟，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如出现沉淀，应再次离心。2. 血浆：应根据标本的要求选择edta或柠檬酸钠作为抗凝剂，混合10-20分钟后，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如有沉淀形成，应该再次离心。3. 尿液：用无菌管收集，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如有沉淀形成，应再次离心。胸腹水、脑脊液参照实行。4. 细胞培养上清：检测分泌性的成份时，用无菌管收集。离心20分钟左右（2000-3000转/分）。仔细收集上清。检测细胞内的成份时，用pbs（ph7.2-7.4）稀释细胞悬液，细胞浓度达到100万/ml左右。通过反复冻融，以使细胞破坏并放出细胞内成份。离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。5. 组织标本：切割标本后，称取重量。加入一定量的pbs，ph7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的pbs（ph7.4），用手工或匀浆器将标本匀浆充分。离心20分钟左右（2000-3000转/分）。仔细收集上清。分装后一份待检测，其余冷冻备用。6. 标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融.7. 不能检测含nan3的样品，因nan3抑制辣根过氧化物酶的（hrp）活性。 操作步骤：1. 标准品的稀释与加样：在酶标包被板上设标准品孔10孔，在第一、第二孔中分别加标准品100μl，然后在第一、第二孔中加标准品稀释液50μl，混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液50μl，混匀；然后在第三孔和第四孔中先各取50μl弃掉，再各取50μl分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液50ul，混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液50μl，混匀后从第七、第八孔中分别取50μl加到第九、第十孔中，再在第九第十孔分别加标准品稀释液50μl，混匀后从第九第十孔中各取50μl弃掉。（稀释后各孔加样量都为50μl，浓度分别为1200 ng/l，800ng/l ，400ng/l，200ng/l，100ng/l）。2. 加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40μl，然后再加待测样品10μl（样品最终稀释度为5倍）。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。3. 温育：用封板膜封板后置37℃温育30分钟。4. 配液：将30（48t的20倍）倍浓缩洗涤液用蒸馏水30（48t的20倍）倍稀释后备用。5. 洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30秒后弃去，如此重复5次，拍干。6. 加酶：每孔加入酶标试剂50μl，空白孔除外。7. 温育：操作同3。8. 洗涤：操作同5。9. 显色：每孔先加入显色剂a50μl，再加入显色剂b50μl，轻轻震荡混匀，37℃避光显色15分钟. 10. 终止：每孔加终止液50μl，终止反应（此时蓝色立转黄色）。11. 测定：以空白空调零，450nm波长依序测量各孔的吸光度（od值）。 测定应在加终止液后15分钟以内进行。 注意事项：1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。2． 浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。3． 各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。一次加样时间最好控制在5分钟内，如标本数量多，推荐使用排枪加样。4． 请每次测定的同时做标准曲线，最好做复孔。如标本中待测物质含量过高（样本od值大于标准品孔第一孔的od值），请先用样品稀释液稀释一定倍数（n倍）后再测定，计算时请最后乘以总稀释倍数（×n×5）。5． 封板膜只限一次性使用，以避免交叉污染。6． 底物请避光保存。7． 严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准.8． 所有样品，洗涤液和各种废弃物都应按传染物处理。9． 本试剂不同批号组分不得混用。10. 如与英文说明书有异，以英文说明书为准。  计算：以标准物的浓度为横坐标，od值为纵坐标，    在坐标纸上绘出标准曲线，根据样品的od      值由标准曲线查出相应的浓度；再乘以稀释      倍数；或用标准物的浓度与od值计算出标      准曲线的直线回归方程式，将样品的od值      代入方程式，计算出样品浓度，再乘以稀释      倍数，即为样品的实际浓度。                                                                   （此图仅供参考）   试剂盒性能：1.样品线性回归与预期浓度相关系数r值为0.95以上。2.批内与批间应分别小于9%和11%  检测范围：                                              50 ng/l -1500ng/l                                                                  保存条件及有效期：1.试剂盒保存：；2-8℃。2．有效期：6个月      

APOA1 Human/重组人载脂蛋白A-ISynonymsApolipoprotein A-I, Apo-AI, ApoA-I, APOA1, MGC117399.IntroductionAPOA1 (Apolipoprotein A-1) is a human protein with a specific role in lipid metabolism being the main protein component of HDL in the plasma. APOA1 promotes cholesterol efflux from tissues to the liver for excretion. Furthermore, APOA1 is a cofactor for LCAT, which is responsible for the formation of most plasma cholesteryl esters. In addition, APOA1 activates spermatozoa motility as part of the SPAP complex. The APOA1 gene is strongly linked with two other apolipoprotein genes on chromosome 11. Defects in the APOA1 gene are linked to HDL deficiency including Tangier disease, and with systemic non-neuropathic amyloidosis. High levels of APOA1 are linked to the manifestation of asthma and atopy.DescriptionApolipoprotein A-I Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 244 amino acids and having a molecular mass of 28.2kDa.The APOA1 is purified by proprietary chromatographic techniques.SourceEscherichia Coli.Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.FormulationThe APOA1 protein was lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.0.SolubilityIt is recommended to reconstitute the lyophilized APOA1 in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.StabilityLyophilized Apolipoprotein A-I although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution APOA1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.PurityGreater than 97.0% as determined by: (a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.Amino Acid SequenceMDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ.UsageProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

植物渗调蛋白（osmotins)elisa试剂盒说明书植物渗调蛋白（osmotins)酶联免疫分析（elisa）试剂盒使用说明书本试剂仅供研究使用       目的：本试剂盒用于测定植物组织，细胞及其它相关样本中渗调蛋白（osmotins)含量。实验原理：   本试剂盒应用双抗体夹心法测定标本中植物渗调蛋白（osmotins)水平。用纯化的植物渗调蛋白（osmotins)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入渗调蛋白（osmotins），再与hrp标记的渗调蛋白（osmotins)抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物tmb显色。tmb在hrp酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的渗调蛋白（osmotins)呈正相关。用酶标仪在450nm波长下测定吸光度（od值），通过标准曲线计算样品中植物渗调蛋白（osmotins)浓度。 试剂盒组成：试剂盒组成48孔配置96孔配置保存说明书1份1份封板膜2片（48）2片（96）密封袋1个1个酶标包被板1×481×962-8℃保存标准品：90ng/l0.5ml×1瓶0.5ml×1瓶2-8℃保存标准品稀释液1.5ml×1瓶1.5ml×1瓶2-8℃保存酶标试剂3 ml×1瓶6 ml×1瓶2-8℃保存样品稀释液3 ml×1瓶6 ml×1瓶2-8℃保存显色剂a液3 ml×1瓶6 ml×1瓶2-8℃保存显色剂b液3 ml×1瓶6 ml×1瓶2-8℃保存终止液3ml×1瓶6ml×1瓶2-8℃保存浓缩洗涤液（20ml×20倍）×1瓶（20ml×30倍）×1瓶2-8℃保存 标本要求： 1．标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融2．不能检测含nan3的样品，因nan3抑制辣根过氧化物酶的（hrp）活性。 操作步骤：1. 标准品的稀释与加样：在酶标包被板上设标准品孔10孔，在第一、第二孔中分别加标准品100μl，然后在第一、第二孔中加标准品稀释液50μl，混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液50μl，混匀；然后在第三孔和第四孔中先各取50μl弃掉，再各取50μl分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液50ul，混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液50μl，混匀后从第七、第八孔中分别取50μl加到第九、第十孔中，再在第九第十孔分别加标准品稀释液50μl，混匀后从第九第十孔中各取50μl弃掉。（稀释后各孔加样量都为50μl，浓度分别为60ng/l ，40ng/l，20ng/l，10ng/l，5ng/l)。2. 加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40μl，然后再加待测样品10μl（样品最终稀释度为5倍）。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。3. 温育：用封板膜封板后置37℃温育30分钟。4. 配液：将30（48t的20倍）倍浓缩洗涤液用蒸馏水30（48t的20倍）倍稀释后备用。5. 洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30秒后弃去，如此重复5次，拍干。6. 加酶：每孔加入酶标试剂50μl，空白孔除外。7. 温育：操作同3。8. 洗涤：操作同5。9. 显色：每孔先加入显色剂a50μl，再加入显色剂b50μl，轻轻震荡混匀，37℃避光显色15分钟. 10. 终止：每孔加终止液50μl，终止反应（此时蓝色立转黄色）。11. 测定：以空白空调零，450nm波长依序测量各孔的吸光度（od值）。 测定应在加终止液后15分钟以内进行。 注意事项：1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。2． 浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。3． 各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。一次加样时间最好控制在5分钟内，如标本数量多，推荐使用排枪加样。4． 请每次测定的同时做标准曲线，最好做复孔。如标本中待测物质含量过高（样本od值大于标准品孔第一孔的od值），请先用样品稀释液稀释一定倍数（n倍）后再测定，计算时请最后乘以总稀释倍数（×n×5）。5． 封板膜只限一次性使用，以避免交叉污染。6． 底物请避光保存。7． 严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准.8． 所有样品，洗涤液和各种废弃物都应按传染物处理。9． 本试剂不同批号组分不得混用。10. 如与英文说明书有异，以英文说明书为准。         计算：以标准物的浓度为横坐标，od值为纵坐标，    在坐标纸上绘出标准曲线，根据样品的od      值由标准曲线查出相应的浓度；再乘以稀释      倍数；或用标准物的浓度与od值计算出标      准曲线的直线回归方程式，将样品的od值      代入方程式，计算出样品浓度，再乘以稀释      倍数，即为样品的实际浓度。                                                                   （此图仅供参考）   试剂盒性能：1.样品线性回归与预期浓度相关系数r值为0.95以上。2.批内与批间应分别小于9%和11%  检测范围：                                              2ng/l –80ng/l                                                                     保存条件及有效期：1.试剂盒保存：；2-8℃。2．有效期：6个月

小鼠3型毒蕈碱乙酰胆碱受体（M3R）  Mouse M3R ELISA Kit Catalogue Number: MG3782 (96Tests) Store all reagents at 2-8°C   Collect sample: serum or blood plasma   Assay range  ：  0.6 pg/ml -65pg/ml     FOR LABORATORY RESEARCH USE ONLY.   NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!   PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!      INTENDED USE   This M3R ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of M3R in the sample, this M3R ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus M3R concentration. The concentration of M3R in the samples is then determined by comparing the O.D. of the samples to the standard curve.    PRINCIPLE OF THE ASSAY   The kit assay M3R level in the sample，use Purified antibody to coat microtiter plate wells, make solid-phase antibody, Samples which including standards of known concentrations and unknowns are pipetted into coated microtiter wells, after Incubating ,add Biotinylated anti-IgG,and Combined Streptavidin-HRP, TSZ  become antibody – antigen - enzyme- antibody complex, after washing Completely, Add TMB substrate solution, TMB Chromogen Solution Becomes blue color At HRP enzyme-catalyzed, And at the effect of acid the color finally become yellow, The intensity of this coloured product is directly proportional to the concentration of M3R present in the samples. measure the optical densit (OD) at 450 nm with microtiter plate reader, calculate M3R concentration by standard curve.    REAGENTS PROVIDED   All reagents provided are stored at 2-8° C. Refer to the expiration date on the label.   1. MICROTITER PLATE 96 wells   2. Biotinylated anti-IgG 6.0 mL 1 tube   3. STANDARD(80 pg/ml)    0.5ml 1 tube 4. STREPTAVIDIN-HRP    6.0 mL 1 tube   5. STANDARD DILUENT    1.5ml    1 tube 6. Chromogen Solution A 6.0 mL 1 vial   7. Chromogen Solution B 6.0 mL 1 vial   8. STOP SOLUTION 6.0 mL 1 vial   9. WASH SOLUTION x30 20 mL 1 vial   10. Instruction 1    SAMPLE COLLECTION AND STORAGE   Serum- Use a serum separator tube(SST) and allow samples to clot for 30minutes before centrifugation for 15minutes at approximately 1000 x g.Remove serum and assay immediately or aliquot and store samples at -20 °C or -80°C.   Plasma - Collect plasma using EDTA or heparin as an anticoagulant.Centrifuge samples for 15 minutes at 1000 x g at 2-8°C within 30minutes of collection.Store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.   Cell culture fluid and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.   NOTE: Serum, plasma, and cell culture fluid samples to be used within 7 days may be stored at 2-8°C, otherwise samples must stored at -20°C(≤2months) or -80°C(≤6months) to avoid loss of bioactivity and contamination. Avoid freeze-thaw cycles .When performing the assay slowly bring samples to room temperature.   DO NOT USE HEAT-TREATED SPECIMENS.    MATERIALS REQUIRED BUT NOT SUPPLIED    1. Microplate reader capable of measuring absorbance at 450 nm.   2. Precision pipettes to deliver 2 ml to 1 ml volumes. .   3. Adjustable 10ml -100ml pipettes for reagent preparation.   4. 100 ml and 1 liter graduated cylinders.   5. Calibrated adjustable precision pipettes, preferably with disposable plastic tips. (A manifold multi-channel pipette is desirable for large assays.)   6. Absorbent paper.   7. 37°C incubator.   8. Distilled or deionized water.   9. Data analysis and graphing software..   10. Tubes to prepare standard or sample dilutions.    PRECAUTIONS   1. Do not substitute reagents from one kit lot to another. Standard, conjugate and microtiter plates are matched for optimal performance. Use only the reagents supplied by manufacturer.   2. Allow kit reagents and materials to reach room temperature (20-25°C) before use. Do not use water baths to thaw samples or reagents.   3. Do not use kit components beyond their expiration date.   4. Use only deionized or distilled water to dilute reagents.   5. Do not remove microtiter plate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.   6. Use fresh disposable pipette tips for each transfer to avoid contamination.   7. Do not mix acid and sodium hypochlorite solutions.   8. Serum and plasma should be handled as potentially hazardous and capable of transmitting disease. Disposable gloves must be worn during the assay procedure, since no known test method can offer complete assurance that products derived from human blood will not transmit infectious agents. Therefore, all blood derivatives should be considered potentially infectious and good laboratory practices should be followed.   9. All samples should be disposed of in a manner that will inactivate viruses.   10. Solid Waste: Autoclave 60 min. at 121°C.   11. Liquid Waste: Add sodium hypochlorite to a final concentration of 1.0%. The waste should be allowed to stand for a minimum of 30 minutes to inactivate the viruses before disposal.   12. Substrate Solution is easily contaminated. If bluish prior to use, do not use.   13. Chromogen Solution B contains 20% acetone, keep this reagent away from sources of heat or flame.   14. Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C).    REAGENT PREPARATION  Standard -The Kit provides a stock standard（80 pg/ml）. Allow the standard to sit for a minimum of 5 minutes with gentle mixing prior to making dilutions.Pipette 150μL of Standard Dilution into each tube. (total 5 tubes) Use the 150μL of stock solution to produce a 2-fold dilution series (including 40 pg/ml,20 pg/ml,10 pg/ml,5.0 pg/ml , 2.5 pg/ml). Mix each tube thoroughly before the next transfer.   Wash Buffer - If crystals have formed in the concentrate, warm to room temperature and mix gently until the crystals have completely dissolved. To prepare enough Wash Buffer for one plate, add 20mL Wash Buffer Concentrate into deionized or distilled water to prepare 600mL of Wash Buffer.  ASSAY PROCEDURE   Prepare all Standards before starting assay procedure (see Preparation Reagents). It is recommended that all Standards and Samples be added in duplicate to the Microtiter Plate.   1.  Determine the number of microwell strips required to test the desired number of samples, Each sample, standard and blank should be assayed in duplicate.   2.  add sample: Set blank wells separately (blank comparison wells don’t add sample and ELISA reagent, other each step operation is same). Add 50 μL of Standards or Samples to the appropriate well of the antibody pre-coated Microtiter Plate, and Gently mix. Incubate for 45 min at 37℃ 3.  Configurate liquid: 30 times of wash solution diluted 30 times with distilled water and reserve. 4.  washing:    remove Liquid, dry by swing, add washing buffer to every well, still for 30 second then remove, repeat 4 times. 5.  add Biotinylated anti-IgG: Add diluted Biotinylated anti-IgG 50ul to all wells, Incubate for 30 min at 37℃ 6.  washing:    Operation with 4. 7.  add streptavidin-HRP:    Add streptavidin-HRP 50ul to all wells, Gently mix Incubate for 15 min at 37℃ 8.  washing：Operation with 4. 9.  color:    Add Chromogen Solution A 50ul and Chromogen Solution B to each well,Incubate for 15 min at 37℃ 10. Stop the reaction: Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color Immediately). 11. assay: take blank well as zero , measure the optical densit (OD) at 450 nm after Adding Stop Solution and within 15min.   TYPICAL DATA  CALCULATION OF RESULTS Average the duplicate readings for each standard,control, and sample and subtract the average zero standard optical density. Construct a standard curve by plotting the mean absorbance for each standard on the y-axis against the concentration on the x-axis and draw a best fit curve through the points on the graph. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.                                                       

小鼠3型毒蕈碱乙酰胆碱受体（M3R）  Mouse M3R ELISA Kit Catalogue Number: MG3782 (96Tests) Store all reagents at 2-8°C   Collect sample: serum or blood plasma   Assay range  ：  0.6 pg/ml -65pg/ml     FOR LABORATORY RESEARCH USE ONLY.   NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!   PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!      INTENDED USE   This M3R ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of M3R in the sample, this M3R ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus M3R concentration. The concentration of M3R in the samples is then determined by comparing the O.D. of the samples to the standard curve.    PRINCIPLE OF THE ASSAY   The kit assay M3R level in the sample，use Purified antibody to coat microtiter plate wells, make solid-phase antibody, Samples which including standards of known concentrations and unknowns are pipetted into coated microtiter wells, after Incubating ,add Biotinylated anti-IgG,and Combined Streptavidin-HRP, TSZ  become antibody – antigen - enzyme- antibody complex, after washing Completely, Add TMB substrate solution, TMB Chromogen Solution Becomes blue color At HRP enzyme-catalyzed, And at the effect of acid the color finally become yellow, The intensity of this coloured product is directly proportional to the concentration of M3R present in the samples. measure the optical densit (OD) at 450 nm with microtiter plate reader, calculate M3R concentration by standard curve.    REAGENTS PROVIDED   All reagents provided are stored at 2-8° C. Refer to the expiration date on the label.   1. MICROTITER PLATE 96 wells   2. Biotinylated anti-IgG 6.0 mL 1 tube   3. STANDARD(80 pg/ml)    0.5ml 1 tube 4. STREPTAVIDIN-HRP    6.0 mL 1 tube   5. STANDARD DILUENT    1.5ml    1 tube 6. Chromogen Solution A 6.0 mL 1 vial   7. Chromogen Solution B 6.0 mL 1 vial   8. STOP SOLUTION 6.0 mL 1 vial   9. WASH SOLUTION x30 20 mL 1 vial   10. Instruction 1    SAMPLE COLLECTION AND STORAGE   Serum- Use a serum separator tube(SST) and allow samples to clot for 30minutes before centrifugation for 15minutes at approximately 1000 x g.Remove serum and assay immediately or aliquot and store samples at -20 °C or -80°C.   Plasma - Collect plasma using EDTA or heparin as an anticoagulant.Centrifuge samples for 15 minutes at 1000 x g at 2-8°C within 30minutes of collection.Store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.   Cell culture fluid and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.   NOTE: Serum, plasma, and cell culture fluid samples to be used within 7 days may be stored at 2-8°C, otherwise samples must stored at -20°C(≤2months) or -80°C(≤6months) to avoid loss of bioactivity and contamination. Avoid freeze-thaw cycles .When performing the assay slowly bring samples to room temperature.   DO NOT USE HEAT-TREATED SPECIMENS.    MATERIALS REQUIRED BUT NOT SUPPLIED    1. Microplate reader capable of measuring absorbance at 450 nm.   2. Precision pipettes to deliver 2 ml to 1 ml volumes. .   3. Adjustable 10ml -100ml pipettes for reagent preparation.   4. 100 ml and 1 liter graduated cylinders.   5. Calibrated adjustable precision pipettes, preferably with disposable plastic tips. (A manifold multi-channel pipette is desirable for large assays.)   6. Absorbent paper.   7. 37°C incubator.   8. Distilled or deionized water.   9. Data analysis and graphing software..   10. Tubes to prepare standard or sample dilutions.    PRECAUTIONS   1. Do not substitute reagents from one kit lot to another. Standard, conjugate and microtiter plates are matched for optimal performance. Use only the reagents supplied by manufacturer.   2. Allow kit reagents and materials to reach room temperature (20-25°C) before use. Do not use water baths to thaw samples or reagents.   3. Do not use kit components beyond their expiration date.   4. Use only deionized or distilled water to dilute reagents.   5. Do not remove microtiter plate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.   6. Use fresh disposable pipette tips for each transfer to avoid contamination.   7. Do not mix acid and sodium hypochlorite solutions.   8. Serum and plasma should be handled as potentially hazardous and capable of transmitting disease. Disposable gloves must be worn during the assay procedure, since no known test method can offer complete assurance that products derived from human blood will not transmit infectious agents. Therefore, all blood derivatives should be considered potentially infectious and good laboratory practices should be followed.   9. All samples should be disposed of in a manner that will inactivate viruses.   10. Solid Waste: Autoclave 60 min. at 121°C.   11. Liquid Waste: Add sodium hypochlorite to a final concentration of 1.0%. The waste should be allowed to stand for a minimum of 30 minutes to inactivate the viruses before disposal.   12. Substrate Solution is easily contaminated. If bluish prior to use, do not use.   13. Chromogen Solution B contains 20% acetone, keep this reagent away from sources of heat or flame.   14. Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C).    REAGENT PREPARATION  Standard -The Kit provides a stock standard（80 pg/ml）. Allow the standard to sit for a minimum of 5 minutes with gentle mixing prior to making dilutions.Pipette 150μL of Standard Dilution into each tube. (total 5 tubes) Use the 150μL of stock solution to produce a 2-fold dilution series (including 40 pg/ml,20 pg/ml,10 pg/ml,5.0 pg/ml , 2.5 pg/ml). Mix each tube thoroughly before the next transfer.   Wash Buffer - If crystals have formed in the concentrate, warm to room temperature and mix gently until the crystals have completely dissolved. To prepare enough Wash Buffer for one plate, add 20mL Wash Buffer Concentrate into deionized or distilled water to prepare 600mL of Wash Buffer.  ASSAY PROCEDURE   Prepare all Standards before starting assay procedure (see Preparation Reagents). It is recommended that all Standards and Samples be added in duplicate to the Microtiter Plate.   1.  Determine the number of microwell strips required to test the desired number of samples, Each sample, standard and blank should be assayed in duplicate.   2.  add sample: Set blank wells separately (blank comparison wells don’t add sample and ELISA reagent, other each step operation is same). Add 50 μL of Standards or Samples to the appropriate well of the antibody pre-coated Microtiter Plate, and Gently mix. Incubate for 45 min at 37℃ 3.  Configurate liquid: 30 times of wash solution diluted 30 times with distilled water and reserve. 4.  washing:    remove Liquid, dry by swing, add washing buffer to every well, still for 30 second then remove, repeat 4 times. 5.  add Biotinylated anti-IgG: Add diluted Biotinylated anti-IgG 50ul to all wells, Incubate for 30 min at 37℃ 6.  washing:    Operation with 4. 7.  add streptavidin-HRP:    Add streptavidin-HRP 50ul to all wells, Gently mix Incubate for 15 min at 37℃ 8.  washing：Operation with 4. 9.  color:    Add Chromogen Solution A 50ul and Chromogen Solution B to each well,Incubate for 15 min at 37℃ 10. Stop the reaction: Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color Immediately). 11. assay: take blank well as zero , measure the optical densit (OD) at 450 nm after Adding Stop Solution and within 15min.   TYPICAL DATA  CALCULATION OF RESULTS Average the duplicate readings for each standard,control, and sample and subtract the average zero standard optical density. Construct a standard curve by plotting the mean absorbance for each standard on the y-axis against the concentration on the x-axis and draw a best fit curve through the points on the graph. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.                                                       

sRANKL Mouse 重组小鼠可溶性RANK配体/sRANKL MouseSynonymsSoluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE, TNF-related activation-induced cytokine, OPGL, ODF, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, RANKL, Osteoprotegerin ligand, CD254 antigen, sRANKL, sOdf.IntroductionRANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.DescriptionsRANKL Mouse Recombinant produced in E.coli is single, non-glycosylated, polypeptide chain containing 174 amino acids and having a total molecular mass of 19.9kDa.CD254 is purified by proprietary chromatographic techniques.SourceEscherichia Coli.Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.FormulationThe protein (1mg/ml) was lyophilized with 10mM Na2PO4, pH 7.5 & 50mM NaCl.SolubilityIt is recommended to reconstitute the lyophilized sRANKL in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.StabilityLyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.Amino acid sequencePAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID.PurityGreater than 95.0% as determined by SDS-PAGE.Biological ActivityMeasured by its ability to induce osteoclast formation on murine RAW264.7 cells using a concentration of 50ng/ml shown in “Corning® Osteo Assay Surface 24 Well Plates with Transwell® Permeable Supports- A Useful Tool for Co-Culture Studies” by Rebecca M. Wood and Mark Rothenber, corresponding to a specific activity of 20,000Units/mg.UsageProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

farnesyltransferase inhibitor alx-290-005-m001product specificationalternative name:b581sequence:n-[(2s)-((2r)-amino-3-mercaptopropylamino)-3-methylbutyl]-phe-met-ohformula:c22h38n4o3s2mw:470.7cas:149759-96-6purity:≥90% (hplc)appearance:white to off-white powder.solubility:soluble in water or dmso.shipping:ambientlong term storage:-20°chandling:hygroscopic.

S100A8重组人S100钙结合蛋白A8/S100A8 Human火热促销中SynonymsCalgranulin A, MRP8, CAGA, CGLA, CFAG, Protein S100-A8, S100 calcium-binding protein A8, Migration inhibitory factor-related protein 8, MRP-8, p8, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Calprotectin L1L subunit, Urinary stone protein band A, S100A8, MIF, NIF, L1Ag, CP-10, MA387, 60B8AG.IntroductionS100A8 is a part of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a broad range of cells, and participate in the regulation of cellular processes such as cell cycle progression and differentiation. S100A8 plays a role in the inhibition of casein kinase and as a cytokine. S100A8 altered expression is related with cystic fibrosis disease. S100A8 is a calcium-binding protein that has antimicrobial activity against bacteria and fungi.S100A8 is crucial for resistance towards invasion by pathogenic bacteria. S100A8 up-regulates transcription of genes that are under the control of NF-kappa-B. S100A8 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide. S100A8 endorses tubulin polymerization and promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A8 takes part as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.DescriptionS100A8 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 93 amino acids (1-93 a.a.) and having a molecular mass of 10.8 kDa. The S100A8 is purified by proprietary chromatographic techniques.SourceEscherichia Coli.Physical AppearanceSterile Filtered clear colorless solution.FormulationThe S100A8 solution contains 20mM Tris-HCl pH-8, 1mM DTT, and 10% glycerol.StabilityStore at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.Amino acid sequenceMLTELEKALN SIIDVYHKYS LIKGNFHAVY RDDLKKLLET ECPQYIRKKG ADVWFKELDI NTDGAVNFQE FLILVIKMGV AAHKKSHEES HKE.PurityGreater than 90% as determined by SDS-PAGE.UsageProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.