产品名称： 大鼠肌酸激酶同工酶MM(CK-MM)ELISA Kit    英文名称： Rat Creatine Kinase MM isoenzyme,CK-MM ELISA Kit    货号： CSB-E14405r    规格： 96T    种属： Rat    待测物名称： Creatine Kinase MM isoenzyme,CK-MM    缩写： CK-MM    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm      用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  小鼠肌酸激酶同工酶MM(CK-MM)ELISA Kit  Mouse Creatine Kinase MM isoenzyme,CK-MM ELISA Kit  CSB-E14407m  96T            大鼠肌酸激酶同工酶MB(CK-MB)ELISA Kit  Rat Creatine Kinase MB isoenzyme,CK-MB ELISA Kit  CSB-E14403r  96T            小鼠肌酸激酶同工酶MB(CK-MB)ELISA Kit  Mouse Creatine Kinase MB isoenzyme,CK-MB ELISA Kit  CSB-E14404m  96T            大鼠五聚素3 (PTX3)ELISA Kit  Rat Pentraxin 3 (PTX3)ELISA Kit  CSB-E14401r  96T            兔子核因子-κB亚基p65亲和肽(NF-κB p65)ELISA Kit  Rabbit nuclear factor-κB p65,NF-κB p65 ELISA Kit  CSB-E14402Rb  96T            绵羊免疫球蛋白G(IgG)ELISA Kit  Sheep Immunoglobulin G,IgG ELISA Kit  CSB-E14400Sh  96T            人肌酸激酶同工酶MM(CK-MM)ELISA Kit  Human Creatine Kinase MM isoenzyme(CK-MM) ELISA Kit  CSB-E18038h  96T            小鼠肌酸激酶(CK)ELISA Kit  Mouse Creatine Kinase,CK ELISA Kit  CSB-E14409m  96T

产品名称： 小鼠肌酸激酶同工酶MM(CK-MM)ELISA Kit    英文名称： Mouse Creatine Kinase MM isoenzyme,CK-MM ELISA Kit    货号： CSB-E14407m    规格： 96T    种属： Mouse    待测物名称： Creatine Kinase MM isoenzyme,CK-MM    缩写： CK-MM    样本类型： serum, plasma    检测范围： 0.156 mU/ml-10 mU/ml    灵敏度： 0.039 mU/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm      用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  大鼠肌酸激酶同工酶MB(CK-MB)ELISA Kit  Rat Creatine Kinase MB isoenzyme,CK-MB ELISA Kit  CSB-E14403r  96T            小鼠肌酸激酶同工酶MB(CK-MB)ELISA Kit  Mouse Creatine Kinase MB isoenzyme,CK-MB ELISA Kit  CSB-E14404m  96T            大鼠五聚素3 (PTX3)ELISA Kit  Rat Pentraxin 3 (PTX3)ELISA Kit  CSB-E14401r  96T            兔子核因子-κB亚基p65亲和肽(NF-κB p65)ELISA Kit  Rabbit nuclear factor-κB p65,NF-κB p65 ELISA Kit  CSB-E14402Rb  96T            绵羊免疫球蛋白G(IgG)ELISA Kit  Sheep Immunoglobulin G,IgG ELISA Kit  CSB-E14400Sh  96T            大鼠肌酸激酶同工酶MM(CK-MM)ELISA Kit  Rat Creatine Kinase MM isoenzyme,CK-MM ELISA Kit  CSB-E14405r  96T            人肌酸激酶同工酶MM(CK-MM)ELISA Kit  Human Creatine Kinase MM isoenzyme(CK-MM) ELISA Kit  CSB-E18038h  96T            小鼠肌酸激酶(CK)ELISA Kit  Mouse Creatine Kinase,CK ELISA Kit  CSB-E14409m  96T

产品名称： 人肌酸激酶同工酶MM(CK-MM)ELISA Kit    英文名称： Human Creatine Kinase MM isoenzyme(CK-MM)ELISA Kit    货号： CSB-E18038h    规格： 96T    种属： Human    待测物名称： Creatine Kinase MM isoenzyme(CK-MM)    缩写： CK-MM    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the CK-MM concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.     相关产品：   产品名称 英文名称 货号 规格  小鼠肌酸激酶同工酶MM(CK-MM)ELISA Kit  Mouse Creatine Kinase MM isoenzyme,CK-MM ELISA Kit  CSB-E14407m  96T            马促肾上腺皮质激素(ACTH)ELISA Kit  Horse adrenocorticotropic hormone (ACTH) ELISA kit  CSB-E18039Hs  96T            犬25羟基维生素D3(25 HVD3)ELISA Kit  Dog 25-hydroxy vitamin D3(25 HVD3)ELISA Kit  CSB-E18032c  96T            人alpha突触核蛋白（淀粉样前体非A4成分蛋白）低聚物 (SNCA oligomer) ELISA kit  Human synuclein, alpha (non A4 component of amyloid precursor) oligomer (SNCA oligomer) ELISA kit  CSB-E18033h  96T            鱼黑色素ELISA kit  Fish melanin ELISA kit  CSB-E18034Fh  96T            大鼠肌酸激酶同工酶MM(CK-MM)ELISA Kit  Rat Creatine Kinase MM isoenzyme,CK-MM ELISA Kit  CSB-E14405r  96T

产品名称： 鸡前列腺素F2α(PGF2α)ELISA Kit    英文名称： Chicken Prostaglandin F2α,PGF2α ELISA Kit    货号： CSB-E17743C    规格： 96T    种属： Chicken    待测物名称： Prostaglandin F2α (PGF2α)    缩写： PGF2α    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm      用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  兔前列腺素F2α(PGF2α)ELISA kit  Rabbit prostaglandin F2α(PGF2α) ELISA kit  CSB-EQ027919RB  96T            牛前列腺素F2α(PGF2α)ELISA Kit  Bovine Prostaglandin F2α,PGF2α ELISA Kit  CSB-E13837B  96T            人前列腺素F2α(PGF2α)ELISA Kit  Human Prostaglandin F2α,PGF2α ELISA Kit  CSB-E10142h  96T            大鼠前列腺素F2α(PGF2α)ELISA Kit  Rat Prostaglandin F2α,PGF2α ELISA Kit  CSB-E10256r  96T            猪抗中性粒细胞弹性蛋白酶抗体(ANEA)ELISA Kit  Pig anti-neutrophil elastase antibody(ANEA)ELISA Kit  CSB-E17747p  96T            牛亚硫酸盐氧化酶(sulfite oxidase,SO) ELISA Kit  Bovine Sulfite Oxidase (sulfite oxidase,SO) ELISA Kit  CSB-E17746B  96T            犬血清淀粉样蛋白A2(SAA2)ELISA Kit  Canine serum amyloid A2(SAA2)ELISA Kit  CSB-E17749c  96T            犬血清淀粉样蛋白A(SAA)ELISA Kit  Dog serum amyloid A(SAA)ELISA Kit  CSB-E17748c  96T            猪前列腺素F2α(PGF2α)ELISA Kit  Pig Prostaglandin F2α (PGF2α) ELISA Kit  CSB-E15800p  96T            马前列腺素F2α(PGF2α)ELISA Kit  Horse Prostaglandin F2α(PGF2α)ELISA Kit  CSB-E16488Hs  96T            犬前列腺素F2α(PGF2α)ELISA Kit  Canine Prostaglandin F2α(PGF2α)ELISA Kit  CSB-E16180c  96T            牛氧杂蒽氧化酶ELISA Kit  Bovine xanthene oxidase ELISA Kit  CSB-E17744B  96T            牛醛氧化酶(aldehyde oxidases,AO) ELISA Kit  Bovine aldehyde oxidase I (aldehyde oxidases,AO) ELISA Kit  CSB-E17745B  96T

产品名称： 马前列腺素F2α(PGF2α)ELISA Kit    英文名称： Horse Prostaglandin F2α,PGF2α ELISA Kit    货号： CSB-E16488Hs    规格： 96T    种属： Horse    待测物名称： Prostaglandin F2α(PGF2α)    缩写： PGF2α    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm      用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  兔前列腺素F2α(PGF2α)ELISA kit  Rabbit prostaglandin F2α(PGF2α) ELISA kit  CSB-EQ027919RB  96T            牛前列腺素F2α(PGF2α)ELISA Kit  Bovine Prostaglandin F2α,PGF2α ELISA Kit  CSB-E13837B  96T            人前列腺素F2α(PGF2α)ELISA Kit  Human Prostaglandin F2α,PGF2α ELISA Kit  CSB-E10142h  96T            大鼠前列腺素F2α(PGF2α)ELISA Kit  Rat Prostaglandin F2α,PGF2α ELISA Kit  CSB-E10256r  96T            猪前列腺素F2α(PGF2α)ELISA Kit  Pig Prostaglandin F2α (PGF2α) ELISA Kit  CSB-E15800p  96T            犬前列腺素F2α(PGF2α)ELISA Kit  Canine Prostaglandin F2α(PGF2α)ELISA Kit  CSB-E16180c  96T            鸡前列腺素F2α(PGF2α)ELISA Kit  Chicken Prostaglandin F2α (PGF2α) ELISA Kit  CSB-E17743C  96T

产品名称： 猪前列腺素F2α(PGF2α)ELISA Kit    英文名称： Pig Prostaglandin F2α,PGF2α ELISA Kit    货号： CSB-E15800p    规格： 96T    种属： Pig    待测物名称： Prostaglandin F2α (PGF2α)    缩写： PGF2α    检测范围： 0.8 ng/ml-200 ng/ml    灵敏度： 0.5 ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm      用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  兔前列腺素F2α(PGF2α)ELISA kit  Rabbit prostaglandin F2α(PGF2α) ELISA kit  CSB-EQ027919RB  96T            牛前列腺素F2α(PGF2α)ELISA Kit  Bovine Prostaglandin F2α,PGF2α ELISA Kit  CSB-E13837B  96T            人前列腺素F2α(PGF2α)ELISA Kit  Human Prostaglandin F2α,PGF2α ELISA Kit  CSB-E10142h  96T            猪催乳素(PRL)ELISA Kit  Pig prolactin (PRL) ELISA Kit  CSB-E15801p  96T            大鼠前列腺素F2α(PGF2α)ELISA Kit  Rat Prostaglandin F2α,PGF2α ELISA Kit  CSB-E10256r  96T            人抗白色念珠菌(C.albicans)IgE抗体 ELISA Kit  Human Candida Albicans(C.albicans)IgE antibody ELISA Kit  CSB-E15809h  96T            猪孕激素/孕酮(PROG)ELISA kit  Pig Progesterone(PROG) ELISA kit  CSB-E15802p  96T            马前列腺素F2α(PGF2α)ELISA Kit  Horse Prostaglandin F2α(PGF2α)ELISA Kit  CSB-E16488Hs  96T            犬前列腺素F2α(PGF2α)ELISA Kit  Canine Prostaglandin F2α(PGF2α)ELISA Kit  CSB-E16180c  96T            鸡前列腺素F2α(PGF2α)ELISA Kit  Chicken Prostaglandin F2α (PGF2α) ELISA Kit  CSB-E17743C  96T            豚鼠前列腺素E2(PGE2) ELISA Kit  Guinea Pig Prostaglandin E2 (PG-E2) ELISA Kit  CSB-E15805Gp  96T            Human Muscle Skeletal Receptor Tyrosine Kinase (MUSK) ELISA Kit  Human Muscle Skeletal Receptor Tyrosine Kinase (MUSK) ELISA Kit  CSB-E15804h  96T            人高糖基化人绒毛膜促性腺激素(hCG)ELISA Kit  Human Hyperglycosylated Chorionic Gonadotropin(hCG) ELISA Kit  CSB-E15803h  96T            大鼠泛素连接酶(E3/UBPL)ELISA Kit  Rat E3/ubiquitin-protein ligase (E3/UBPL) ELISA Kit  CSB-E15808r  96T            大鼠泛素结合酶(E2/UBCE)ELISA Kit  Rat E2/ubiquitin-conjugating enzyme (E2/UBCE) ELISA Kit  CSB-E15807r  96T            大鼠泛素激活酶(E1/UBAE)ELISA Kit  Rat E1/ubiquitin-activating enzyme (E1/UBAE) ELISA Kit  CSB-E15806r  96T

 产品名称： 牛前列腺素F2α(PGF2α)ELISA Kit  英文名称： Bovine Prostaglandin F2α,PGF2α ELISA Kit  货号： CSB-E13837B  规格： 96T  种属： Bovine  待测物名称： Prostaglandin F2α,PGF2α  缩写： PGF2α  样本类型： serum, plasma, tissue homogenates  检测范围： 18.75 pg/ml-1200 pg/ml  灵敏度： 4.7 pg/ml  反应时间： 1-5h  所需样本体积： 50-100ul  检测波长： 450 nm  用途： For research use only. Not for diagnostic use.  相关产品： 产品名称 英文名称 货号 规格  兔前列腺素F2α(PGF2α)ELISA kit  Rabbit prostaglandin F2α(PGF2α) ELISA kit  CSB-EQ027919RB  96T   大鼠异柠檬酸脱氢酶(ICD)ELISA Kit  Rat isocitrate dehydrogenase,ICD ELISA Kit  CSB-E13839r  96T          犬白介素1β(IL-1β) ELISA Kit  Dog Interleukin 1β (IL- 1β) ELISA Kit  CSB-E13836c  96T         牛肺表面活性物质相关蛋白C(SP-C)ELISA Kit  Bovine pulmonary surfactant-associated protein C,SP-C ELISA Kit  CSB-E13835B  96T         牛肺表面活性物质相关蛋白B(SP-B)ELISA Kit  Bovine pulmonary surfactant-associated protein B,SP-B ELISA Kit  CSB-E13834B  96T   人前列腺素F2α(PGF2α)ELISA Kit  Human Prostaglandin F2α,PGF2α ELISA Kit  CSB-E10142h  96T   大鼠抗环胍氨酸肽抗体(Anti-CCP-antibody)ELISA Kit  Rat Anti-Cyclic Citrullinated Peptide Antibody(Anti-CCP-antibody)ELISA Kit  CSB-E13830r  96T   大鼠前列腺素F2α(PGF2α)ELISA Kit  Rat Prostaglandin F2α,PGF2α ELISA Kit  CSB-E10256r  96T   大鼠软骨寡聚基质蛋白(COMP)ELISA Kit  Rat cartilage oligomeric matrix protein,COMP ELISA Kit  CSB-E13833r  96T   大鼠N-甲基-D-天门冬氨酸(NMDA)受体亚单位NR2 ELISA Kit  Rat N-Methyl-D-Aspartate (NMDA) Receptor 2 (NR2) ELISA Kit  CSB-E13831r  96T   猪前列腺素F2α(PGF2α)ELISA Kit  Pig Prostaglandin F2α (PGF2α) ELISA Kit  CSB-E15800p  96T   马前列腺素F2α(PGF2α)ELISA Kit  Horse Prostaglandin F2α(PGF2α)ELISA Kit  CSB-E16488Hs  96T   犬前列腺素F2α(PGF2α)ELISA Kit  Canine Prostaglandin F2α(PGF2α)ELISA Kit  CSB-E16180c  96T   鸡前列腺素F2α(PGF2α)ELISA Kit  Chicken Prostaglandin F2α (PGF2α) ELISA Kit  CSB-E17743C  96T   人瓜氨酸ELISA Kit  Human Citrulline ELISA Kit  CSB-E13832h  96T   人癌症促凝素（CP）ELISA Kit  Human Cancer Procoagulant (CP）ELISA Kit  CSB-E13838h  96T

产品名称： 犬前列腺素F2α(PGF2α)ELISA Kit    英文名称： Canine Prostaglandin F2α,PGF2α ELISA Kit    货号： CSB-E16180c    规格： 96T    种属： Dog    待测物名称： Prostaglandin F2α(PGF2α)    缩写： PGF2α    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm      用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  兔前列腺素F2α(PGF2α)ELISA kit  Rabbit prostaglandin F2α(PGF2α) ELISA kit  CSB-EQ027919RB  96T            小鼠NAD依赖性蛋白脱乙酰酶sirtuin-1(SIRT1)ELISA kit  Mouse NAD-dependent deacetylase sirtuin-1(Sirt1)ELISA Kit  CSB-E16187m  96T            牛前列腺素F2α(PGF2α)ELISA Kit  Bovine Prostaglandin F2α,PGF2α ELISA Kit  CSB-E13837B  96T            人前列腺素F2α(PGF2α)ELISA Kit  Human Prostaglandin F2α,PGF2α ELISA Kit  CSB-E10142h  96T            人白介素37(IL-37) ELISA Kit  Human Interleukin 37(IL-37) ELISA Kit  CSB-E16185h  96T            大鼠前列腺素F2α(PGF2α)ELISA Kit  Rat Prostaglandin F2α,PGF2α ELISA Kit  CSB-E10256r  96T            小鼠核因子E2相关因子2(Nrf2)ELISA Kit  Mouse Nuclear factor erythroid 2-related factor 2 (Nrf2) ELISA Kit  CSB-E16188m  96T            大鼠心型脂肪酸结合蛋白(h-FABP)ELISA Kit  Rat heart fatty acid binding protein(h-FABP)ELISA Kit  CSB-E16184r  96T            牛磷酸化腺苷酸活化蛋白激酶(pAMPK)ELISA Kit  Bovine Phosphorylated adenosine monophosphate activated protein kinase (pAMPK) ELISA Kit  CSB-E16186B  96T            猪前列腺素F2α(PGF2α)ELISA Kit  Pig Prostaglandin F2α (PGF2α) ELISA Kit  CSB-E15800p  96T            马前列腺素F2α(PGF2α)ELISA Kit  Horse Prostaglandin F2α(PGF2α)ELISA Kit  CSB-E16488Hs  96T            鸡前列腺素F2α(PGF2α)ELISA Kit  Chicken Prostaglandin F2α (PGF2α) ELISA Kit  CSB-E17743C  96T            犬心型脂肪酸结合蛋白(h-FABP)ELISA Kit  Canine heart fatty acid binding protein(h-FABP)ELISA Kit  CSB-E16183c  96T

产品名称： 人前列腺素F2α(PGF2α)ELISA Kit    英文名称： Human Prostaglandin F2α,PGF2α ELISA Kit    货号： CSB-E10142h    价格： 3600    规格： 96T    种属： Human    待测物名称： Prostaglandin F2α,PGF2α    缩写： PGF2α    样本类型： serum, plasma, tissue homogenates    检测范围： 25 ng/ml-800 ng/ml    灵敏度： 12.5 ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  牛前列腺素F2α(PGF2α)ELISA Kit  Bovine Prostaglandin F2α,PGF2α ELISA Kit  CSB-E13837B  96T            兔前列腺素F2α(PGF2α)ELISA kit  Rabbit prostaglandin F2α(PGF2α) ELISA kit  CSB-EQ027919RB  96T            大鼠前列腺素F2α(PGF2α)ELISA Kit  Rat Prostaglandin F2α,PGF2α ELISA Kit  CSB-E10256r  96T            人8羟基脱氧鸟苷(8-OHdG)ELISA Kit  Human 8-Hydroxydeoxyguanosine (8-OHdG) ELISA kit  CSB-E10140h  96T            人单胺氧化酶(MAO)ELISA Kit  Human monoamine oxidase,MAO ELISA Kit  CSB-E10144h  96T            大鼠尿激酶(UK)ELISA Kit  Rat urokinase,UK ELISA Kit  CSB-E10141r  96T            猪前列腺素F2α(PGF2α)ELISA Kit  Pig Prostaglandin F2α (PGF2α) ELISA Kit  CSB-E15800p  96T            马前列腺素F2α(PGF2α)ELISA Kit  Horse Prostaglandin F2α(PGF2α)ELISA Kit  CSB-E16488Hs  96T            犬前列腺素F2α(PGF2α)ELISA Kit  Canine Prostaglandin F2α(PGF2α)ELISA Kit  CSB-E16180c  96T            鸡前列腺素F2α(PGF2α)ELISA Kit  Chicken Prostaglandin F2α (PGF2α) ELISA Kit  CSB-E17743C  96T

大鼠前列腺素F2α（PGF2α）酶联免疫分析试剂盒使用说明书 本试剂盒仅供研究使用 产品名称： 大鼠前列腺素F2α(PGF2α)ELISA Kit 英文名称： Rat Prostaglandin F2α,PGF2α ELISA Kit 货号： CSB-E10256r 规格： 96T 种属： Rat 待测物名称： Prostaglandin F2α,PGF2α 缩写： PGF2α 样本类型： serum, plasma, cell culture supernates and tissue homogenates 检测范围： 6.25 pg/ml-400 pg/ml 灵敏度： 1.56 pg/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 特异性：本试剂盒可同时检测天然或重组的大鼠PGF2α，且与其他相关蛋白无交叉反应。 有效期：6个月 预期应用：ELISA法定量测定大鼠血清、血浆、细胞培养上清或其它相关生物液体中PGF2α含量。 说明 1．试剂盒保存：-20℃（较长时间不用时）；2-8℃（频繁使用时）。 2．浓洗涤液低温保存会有盐析出，稀释时可在水浴中加温助溶。 3．中、英文说明书可能会有不一致之处，请以英文说明书为准。 4．刚开启的酶联板孔中可能会含有少许水样物质，此为正常现象，不会对实验结果造成任何影响。 实验原理 用纯化的抗体包被微孔板，制成固相载体，往包被抗PGF2α抗体的微孔中依次加入标本或标准品、生物素化的抗PGF2α抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的PGF2α呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。 试剂盒组成及试剂配制 1. 酶联板（Assay plate ）：一块（96孔）。 2. 标准品（Standard）：2瓶（冻干品）。 3. 样品稀释液（Sample Diluent）：1×20ml/瓶。 4. 生物素标记抗体稀释液（Biotin-antibody Diluent）：1×10ml/瓶。 5. 辣根过氧化物酶标记亲和素稀释液 （HRP-avidin Diluent）：1×10ml/瓶。 6. 生物素标记抗体（Biotin-antibody）：1×120μl/瓶（1：100） 7. 辣根过氧化物酶标记亲和素（HRP-avidin）：1×120μl/瓶（1：100） 8. 底物溶液（TMB Substrate）：1×10ml/瓶。 9. 浓洗涤液（Wash Buffer）：1×20ml/瓶，使用时每瓶用蒸馏水稀释25倍。 10. 终止液（Stop Solution）：1×10ml/瓶（2N H2SO4）。 需要而未提供的试剂和器材 1. 标准规格酶标仪 2. 高速离心机 3. 电热恒温培养箱 4. 干净的试管和Eppendof管 5. 系列可调节移液器及吸头，一次检测样品较多时，最好用多通道移液器 6. 蒸馏水，容量瓶等 标本的采集及保存 1. 血清：全血标本请于室温放置2小时或4℃过夜后于1000 x g离心20分钟，取上清即可检测，或将标本放于-20℃或-80℃保存，但应避免反复冻融。 2. 血浆：可用EDTA或肝素作为抗凝剂，标本采集后30分钟内于2 - 8° C 1000 x g离心15分钟，或将标本放于-20℃或-80℃保存，但应避免反复冻融。 3. 细胞培养物上清或其它生物标本：1000 x g离心20分钟，取上清即可检测，或将标本放于-20℃或-80℃保存，但应避免反复冻融。 注：标本溶血会影响最后检测结果，因此溶血标本不宜进行此项检测。 标本的稀释原则： 首先通过文献检索的方式了解待测样本的大致含量，确定适当的稀释倍数。只有稀释至标准曲线的范围内，检测的结果才是准确的。稀释的过程中，应做好详细的记录。最后计算浓度时，稀释了“N”倍，标本的浓度应再乘以“N”。 标准品的稀释原则：2瓶，每瓶临用前以样品稀释液稀释至1ml，盖好后静置10分钟以上，然后反复颠倒/搓动以助溶解，其浓度为3000 pg/ml，做系列倍比稀释后，分别稀释3000 pg/ml，1500 pg/ml，750 pg/ml，375 pg/ml，187.5 pg/ml，93.8 pg/ml，46.9 pg/ml，样品稀释液直接作为标准浓度0 pg/ml，临用前15分钟内配制。 如配制1500 pg/ml标准品：取0.5ml（不要少于0.5ml）3000 pg/ml的上述标准品加入含0.5ml样品稀释液的Eppendorf管中，混匀即可，其余浓度以此类推。 生物素标记抗体的稀释原则： 临用前以生物素标记抗体稀释液稀释，稀释前根据预先计算好的每次实验所需的总量配制（每孔100μl），实际配制时应多配制0.1-0.2ml。如10μl生物素标记抗体加990μl生物素标记抗体稀释液的比例配制，轻轻混匀，在使用前一小时内配制。 辣根过氧化物酶标记亲和素的稀释原则： 临用前以辣根过氧化物酶标记亲和素稀释液稀释，稀释前根据预先计算好的每次实验所需的总量配制（每孔100μl），实际配制时应多配制0.1-0.2ml。如10μl辣根过氧化物酶标记亲和素加990μl辣根过氧化物酶标记亲和素稀释液 的比例配制，轻轻混匀，在使用前一小时内配制。 操作步骤 实验开始前，请提前配置好所有试剂，试剂或样品稀释时，均需混匀，混匀时尽量避免起泡。每次检测都应该做标准曲线。如样品浓度过高时，用样品稀释液进行稀释，以使样品符合试剂盒的检测范围。 1. 加样：分别设空白孔、标准孔、待测样品孔。空白孔加样品稀释液100μl，余孔分别加标准品或待测样品100μl，注意不要有气泡，加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀，酶标板加上盖或覆膜，37℃反应120分钟。 为保证实验结果有效性，每次实验请使用新的标准品溶液。 2. 弃去液体，甩干，不用洗涤。每孔加生物素标记抗体工作液 100μl（取1μl生物素标记抗体加99μl生物素标记抗体稀释液的比例配制，轻轻混匀，在使用前一小时内配制），37℃,60分钟。 3. 温育60分钟后，弃去孔内液体，甩干，洗板3次，每次浸泡1-2分钟，200μl/每孔，甩干。 4. 每孔加辣根过氧化物酶标记亲和素工作液（同生物素标记抗体工作液） 100μl，37℃，60分钟。 5. 温育60分钟后，弃去孔内液体，甩干，洗板5次，每次浸泡1-2分钟，200μl/每孔，甩干。 6. 依序每孔加底物溶液90μl，37℃避光显色（30分钟内，此时肉眼可见标准品的前3-4孔有明显的梯度蓝色，后3-4孔显色不明显，即可终止）。 7. 依序每孔加终止溶液50μl，终止反应（此时蓝色立转黄色）。终止液的加入顺序应尽量与底物液的加入顺序相同。为了保证实验结果的准确性，底物反应时间到后应尽快加入终止液。 8. 用酶联仪在450nm波长依序测量各孔的光密度（OD值）。 在加终止液后15分钟以内进行检测。 注： 1. 用户在初次使用试剂盒时，应将各种试剂管离心数分钟，以便试剂集中到管底。 2. 每次实验留一孔作为空白调零孔，该孔不加任何试剂，只是最后加底物溶液及2N H2SO4。测量时先用此孔调OD值至零。 3. 为防止样品蒸发，试验时将反应板放于铺有湿布的密闭盒内，酶标板加上盖或覆膜。 4. 未使用完的酶标板或者试剂，请于2-8℃保存。标准品、生物素标记抗体工作液、辣根过氧化物酶标记亲和素工作液请依据所需的量配置使用。请勿重复使用已稀释过的标准品、生物素标记抗体工作液、或辣根过氧化物酶标记亲和素工作液。 5. 建议检测样品时均设双孔测定，以保证检测结果的准确性。 洗板方法 手工洗板方法：吸去（不可触及板壁）或甩掉酶标板内的液体；在实验台上铺垫几层吸水纸，酶标板朝下用力拍几次；将推荐的洗涤缓冲液至少0.3ml注入孔内，浸泡1-2分钟。根据需要，重复此过程数次。 自动洗板：如果有自动洗板机，应在熟练使用后再用到正式实验过程中。 计算 以标准物的浓度为横坐标（对数坐标），OD值为纵坐标（普通坐标），在半对数坐标纸上绘出标准曲线，根据样品的OD值由标准曲线查出相应的浓度；再乘以稀释倍数；或用标准物的浓度与OD值计算出标准曲线的直线回归方程式，将样品的OD值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实际浓度。 注意事项 1. 当混合蛋白溶液时应尽量轻缓，避免起泡。 2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。 3. 一次加样时间最好控制在5分钟内，如标本数量多，推荐使用排枪加样。 4. 请每次测定的同时做标准曲线，最好做复孔。 5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请最后乘以稀释倍数。 6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。 7. 底物请避光保存。 8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。  精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the PGF2α concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 相关产品： 产品名称 英文名称 货号 规格  人前列腺素F2α(PGF2α)ELISA Kit  Human Prostaglandin F2α,PGF2α ELISA Kit  CSB-E10142h  96T            猪前列腺素F2α(PGF2α)ELISA Kit  Pig Prostaglandin F2α (PGF2α) ELISA Kit  CSB-E15800p  96T            马前列腺素F2α(PGF2α)ELISA Kit  Horse Prostaglandin F2α(PGF2α)ELISA Kit  CSB-E16488Hs  96T            犬前列腺素F2α(PGF2α)ELISA Kit  Canine Prostaglandin F2α(PGF2α)ELISA Kit  CSB-E16180c  96T            鸡前列腺素F2α(PGF2α)ELISA Kit  Chicken Prostaglandin F2α (PGF2α) ELISA Kit  CSB-E17743C  96T            牛前列腺素F2α(PGF2α)ELISA Kit  Bovine Prostaglandin F2α,PGF2α ELISA Kit  CSB-E13837B  96T            大鼠白三烯E4(LTE4)ELISA Kit  Rat leukotriene E4,LT-E4 ELISA Kit  CSB-E10255r  96T            小鼠白介素7(IL-7)ELISA Kit  Mouse Interleukin-7,IL-7 ELISA kit  CSB-E10257m  96T

产品名称： 犬5羟色胺(5-HT)ELISA Kit    英文名称： Canine 5-Hydroxytryptamine,5HT ELISA Kit    货号： CSB-E08366c    规格： 96T    种属： Dog    待测物名称： 5-Hydroxytryptamine,5HT    缩写： 5HT    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  鸽子5羟色胺(5-HT)ELISA Kit  Pigeon 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E13388pn  96T            大鼠γ氨基丁酸(GABA)ELISA Kit  Rat Gamma-aminobutyric acid,GABA ELISA Kit  CSB-E08368r  96T            人γ氨基丁酸(GABA)ELISA Kit  Human Gamma-aminobutyric acid,GABA ELISA Kit  CSB-E08367h  96T            大鼠5羟色胺(5-HT)ELISA Kit  Rat 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E08364r  96T            小鼠5羟色胺(5-HT)ELISA Kit  Mouse 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E08365m  96T            人5羟色胺(5-HT)ELISA Kit  Human 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E08363h  96T            大鼠P物质受体(SP-R)ELISA Kit  Rat substance P receptor,SP-R ELISA Kit  CSB-E08361r  96T            小鼠P物质受体(SP-R)ELISA Kit  Mouse substance P receptor,SP-R ELISA Kit  CSB-E08362m  96T            人P物质受体(SP-R)ELISA Kit  Human substance P receptor,SP-R ELISA Kit  CSB-E08360h  96T            小鼠γ氨基丁酸(GABA)ELISA Kit  Mouse Gamma-aminobutyric acid,GABA ELISA Kit  CSB-E08369m  96T            猪5羟色胺(5-HT)ELISA Kit  Pig 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E13560p  96T

产品名称： 人5羟色胺(5-HT)ELISA Kit    英文名称： Human 5-Hydroxytryptamine,5HT ELISA Kit    货号： CSB-E08363h    价格： 3600    规格： 96T    种属： Human    待测物名称： 5-Hydroxytryptamine,5HT    缩写： 5HT    样本类型： serum, plasma, tissue homogenates    检测范围： 4ng/ml-800ng/ml    灵敏度： 2 ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  鸽子5羟色胺(5-HT)ELISA Kit  Pigeon 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E13388pn  96T            大鼠γ氨基丁酸(GABA)ELISA Kit  Rat Gamma-aminobutyric acid,GABA ELISA Kit  CSB-E08368r  96T            人γ氨基丁酸(GABA)ELISA Kit  Human Gamma-aminobutyric acid,GABA ELISA Kit  CSB-E08367h  96T            大鼠5羟色胺(5-HT)ELISA Kit  Rat 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E08364r  96T            小鼠5羟色胺(5-HT)ELISA Kit  Mouse 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E08365m  96T            大鼠P物质受体(SP-R)ELISA Kit  Rat substance P receptor,SP-R ELISA Kit  CSB-E08361r  96T            小鼠P物质受体(SP-R)ELISA Kit  Mouse substance P receptor,SP-R ELISA Kit  CSB-E08362m  96T            人P物质受体(SP-R)ELISA Kit  Human substance P receptor,SP-R ELISA Kit  CSB-E08360h  96T            小鼠γ氨基丁酸(GABA)ELISA Kit  Mouse Gamma-aminobutyric acid,GABA ELISA Kit  CSB-E08369m  96T            猪5羟色胺(5-HT)ELISA Kit  Pig 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E13560p  96T            犬5羟色胺(5-HT)ELISA Kit  Canine 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E08366c  96T

产品名称： 大鼠5羟色胺(5-HT)ELISA Kit    英文名称： Rat 5-Hydroxytryptamine,5HT ELISA Kit    货号： CSB-E08364r    规格： 96T    种属： Rat    待测物名称： 5-Hydroxytryptamine,5HT    缩写： 5HT    样本类型： serum, plasma, cell lysates, tissue homogenates    检测范围： 0.8 ng/ml-160 ng/ml    灵敏度： 0.4 ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.   产品名称 英文名称 货号 规格  鸽子5羟色胺(5-HT)ELISA Kit  Pigeon 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E13388pn  96T            大鼠γ氨基丁酸(GABA)ELISA Kit  Rat Gamma-aminobutyric acid,GABA ELISA Kit  CSB-E08368r  96T            人γ氨基丁酸(GABA)ELISA Kit  Human Gamma-aminobutyric acid,GABA ELISA Kit  CSB-E08367h  96T            小鼠5羟色胺(5-HT)ELISA Kit  Mouse 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E08365m  96T            人5羟色胺(5-HT)ELISA Kit  Human 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E08363h  96T            大鼠P物质受体(SP-R)ELISA Kit  Rat substance P receptor,SP-R ELISA Kit  CSB-E08361r  96T            小鼠P物质受体(SP-R)ELISA Kit  Mouse substance P receptor,SP-R ELISA Kit  CSB-E08362m  96T            人P物质受体(SP-R)ELISA Kit  Human substance P receptor,SP-R ELISA Kit  CSB-E08360h  96T            小鼠γ氨基丁酸(GABA)ELISA Kit  Mouse Gamma-aminobutyric acid,GABA ELISA Kit  CSB-E08369m  96T            猪5羟色胺(5-HT)ELISA Kit  Pig 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E13560p  96T            犬5羟色胺(5-HT)ELISA Kit  Canine 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E08366c  96T

产品名称： 小鼠5羟色胺(5-HT)ELISA Kit    英文名称： Mouse 5-Hydroxytryptamine,5HT ELISA Kit    货号： CSB-E08365m    规格： 96T    种属： Mouse    待测物名称： 5-Hydroxytryptamine,5HT    缩写： 5HT    样本类型： serum, plasma, tissue homogenates    检测范围： 250 pg/ml-4000pg/ml    灵敏度： 125 pg/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  鸽子5羟色胺(5-HT)ELISA Kit  Pigeon 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E13388pn  96T            大鼠γ氨基丁酸(GABA)ELISA Kit  Rat Gamma-aminobutyric acid,GABA ELISA Kit  CSB-E08368r  96T            人γ氨基丁酸(GABA)ELISA Kit  Human Gamma-aminobutyric acid,GABA ELISA Kit  CSB-E08367h  96T            大鼠5羟色胺(5-HT)ELISA Kit  Rat 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E08364r  96T            人5羟色胺(5-HT)ELISA Kit  Human 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E08363h  96T            大鼠P物质受体(SP-R)ELISA Kit  Rat substance P receptor,SP-R ELISA Kit  CSB-E08361r  96T            小鼠P物质受体(SP-R)ELISA Kit  Mouse substance P receptor,SP-R ELISA Kit  CSB-E08362m  96T            人P物质受体(SP-R)ELISA Kit  Human substance P receptor,SP-R ELISA Kit  CSB-E08360h  96T            小鼠γ氨基丁酸(GABA)ELISA Kit  Mouse Gamma-aminobutyric acid,GABA ELISA Kit  CSB-E08369m  96T            猪5羟色胺(5-HT)ELISA Kit  Pig 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E13560p  96T            犬5羟色胺(5-HT)ELISA Kit  Canine 5-Hydroxytryptamine,5HT ELISA Kit  CSB-E08366c  96T

 产品名称： 鸡H5型禽流感病毒(H5N1)抗体(IgG)ELISA kit    英文名称： Chicken H5N1 antibody(IgG) ELISA kit    货号： CSB-EQ027217CH    价格： 4200    规格： 96T    种属： Chicken    待测物名称： H5N1 antibody(IgG)    样本类型： serum    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use

 产品名称： 鸡新城疫病毒(NDV)抗体 ELISA kit    英文名称： Chicken newcastle disease virus(NDV) antibody ELISA kit    货号： CSB-EQ027216CH    价格： 4200    规格： 96T    种属： Chicken    待测物名称： newcastle disease virus(NDV) antibody (IgG)    样本类型： serum, plasma    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm  用途： For research use only. Not for diagnostic use.

 产品名称： 小鼠γ干扰素(IFN-γ)ELISA Kit    英文名称： Mouse Interferon γ ,IFN-γ ELISA Kit    别名： IFG, IFI    货号： CSB-E04578m    价格： 3200    规格： 96T    种属： Mouse    待测物名称： interferon, gamma    缩写： IFNG    样本类型： serum, plasma, tissue homogenates and cell culture supernates    检测范围： 15.6 pg/ml-1000 pg/ml    灵敏度： 3.9 pg/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFNG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFNG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFNG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFNG bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Mouse IFNG. No significant cross-reactivity or interference between Mouse IFNG and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IFNG concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   相关产品：   产品名称 英文名称 货号 规格  山羊γ干扰素(IFN-γ)ELISA Kit  Goat Interferon γ ,IFN-γ ELISA Kit  CSB-E12966G  96T            仓鼠γ干扰素(IFNG)ELISA kit  Hamster interferon, gamma (IFNG) ELISA kit  CSB-EL011050HA  96T            猪γ干扰素(IFN-γ)ELISA Kit  Pig Interferon γ ,IFN-γ ELISA Kit  CSB-E06794p  96T            牛γ干扰素(IFN-γ)ELISA Kit  Bovine Interferon γ ,IFN-γ ELISA Kit  CSB-E09812b  96T            鸭γ干扰素(IFNG) ELISA kit  Duck interferon, gamma (IFNG) ELISA kit  CSB-EL011050DU  96T            大鼠γ干扰素(IFN-γ)ELISA Kit  Rat Interferon γ ,IFN-γ ELISA Kit  CSB-E04579r  96T            鱼γ干扰素(IFN-γ)ELISA Kit  Fish Interferon γ ,IFN-γ ELISA Kit  CSB-E12878Fh  96T            绵羊γ干扰素(IFN-γ)ELISA Kit  Sheep Interferon γ ,IFN-γ ELISA Kit  CSB-E14018Sh  96T            人γ干扰素(IFN-γ)ELISA Kit  Human Interferon γ ,IFN-γ ELISA Kit  CSB-E04577h  96T            兔子γ干扰素(IFN-γ)ELISA Kit  rabbit Interferon γ ,IFN-γ ELISA Kit  CSB-E06925Rb  96T            鸡γ干扰素(IFN-γ)ELISA Kit  Chicken Interferon γ ,IFN-γ ELISA Kit  CSB-E08550Ch  96T            鹿γ干扰素(IFNG) ELISA kit  Deer interferon, gamma (IFNG) ELISA kit  CSB-EL011050DE  96T            猴γ干扰素(IFN-γ)ELISA Kit  Monkey Interferon γ ,IFN-γ ELISA Kit  CSB-E10049Mo  96T            Horse Interferon gamma(IFNG) ELISA kit  Horse Interferon gamma(IFNG) ELISA kit  CSB-EL011050HO  96T            Donkey Interferon gamma(IFNG) ELISA kit  Donkey Interferon gamma(IFNG) ELISA kit  CSB-EL011050DK  96T            犬γ干扰素(IFN-γ)ELISA Kit  Dog Interferon γ ,IFN-γ ELISA Kit  CSB-E11262c  96T            Cat Interferon gamma(IFNG) ELISA kit  Cat Interferon gamma(IFNG) ELISA kit  CSB-EL011050CA  96T           抗体  产品名称 货号 规格  Rabbit anti-human interferon, gamma polyclonal Antibody  CSB-PA011050GA01HU  150ul         蛋白  产品名称 货号 规格 蛋白来源  Recombinant Meleagris gallopavo Interferon gamma(IFNG)  1mg            Recombinant Coturnix coturnix japonica Interferon gamma(IFNG)  1mg            Recombinant Numida meleagris Interferon gamma(IFNG)  1mg            Recombinant Phasianus colchicus colchicus Interferon gamma(IFNG)  1mg            Recombinant Marmota monax Interferon gamma(IFNG)  1mg            Recombinant Mesocricetus auratus Interferon gamma(IFNG)  1mg            Recombinant Mustela putorius furo Interferon gamma(IFNG)  1mg            Recombinant Rat Interferon gamma(Ifng)  1mg            Recombinant Macaca nemestrina Interferon gamma(IFNG)  1mg            Recombinant Macaca fascicularis Interferon gamma(IFNG)  1mg            Recombinant Callithrix jacchus Interferon gamma(IFNG)  1mg            Recombinant Cercocebus atys Interferon gamma(IFNG)  1mg            Recombinant Cervus elaphus Interferon gamma(IFNG)  1mg            Recombinant Donkey Interferon gamma(IFNG)  1mg            Recombinant Mouse Interferon gamma(Ifng)  1mg            Recombinant Bovine Interferon gamma(IFNG)  1mg            Recombinant Human Interferon gamma(IFNG)  1mg            Recombinant Macaca mulatta (Rhesus macaque) Interferon gamma(IFNG)  1mg            Recombinant Chicken Interferon gamma(IFNG)  1mg            Recombinant Cat Interferon gamma(IFNG)  1mg            Recombinant Horse Interferon gamma(IFNG)  1mg            Recombinant Dog Interferon gamma(IFNG)  1mg            Recombinant Rabbit Interferon gamma(IFNG)  1mg            Recombinant Pig Interferon gamma(IFNG)  1mg            Recombinant Sheep Interferon gamma(IFNG)  1mg            Recombinant Goat Interferon gamma(IFNG)  1mg

 产品名称： 人γ干扰素(IFN-γ)ELISA Kit    英文名称： Human Interferon γ ,IFN-γ ELISA Kit    别名： IFG, IFI    货号： CSB-E04577h    价格： 3200    规格： 96T    种属： Human    待测物名称： interferon, gamma    缩写： IFNG    样本类型： serum, cell culture supernates, urine, tissue homogenates    检测范围： 6.25 pg/ml-400 pg/ml    灵敏度： 1.56 pg/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFNG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFNG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFNG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFNG bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Human IFNG. No significant cross-reactivity or interference between Human IFNG and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IFNG concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   相关产品：   产品名称 英文名称 货号 规格  山羊γ干扰素(IFN-γ)ELISA Kit  Goat Interferon γ ,IFN-γ ELISA Kit  CSB-E12966G  96T            仓鼠γ干扰素(IFNG)ELISA kit  Hamster interferon, gamma (IFNG) ELISA kit  CSB-EL011050HA  96T            猪γ干扰素(IFN-γ)ELISA Kit  Pig Interferon γ ,IFN-γ ELISA Kit  CSB-E06794p  96T            牛γ干扰素(IFN-γ)ELISA Kit  Bovine Interferon γ ,IFN-γ ELISA Kit  CSB-E09812b  96T            鸭γ干扰素(IFNG) ELISA kit  Duck interferon, gamma (IFNG) ELISA kit  CSB-EL011050DU  96T            大鼠γ干扰素(IFN-γ)ELISA Kit  Rat Interferon γ ,IFN-γ ELISA Kit  CSB-E04579r  96T            鱼γ干扰素(IFN-γ)ELISA Kit  Fish Interferon γ ,IFN-γ ELISA Kit  CSB-E12878Fh  96T            绵羊γ干扰素(IFN-γ)ELISA Kit  Sheep Interferon γ ,IFN-γ ELISA Kit  CSB-E14018Sh  96T            小鼠γ干扰素(IFN-γ)ELISA Kit  Mouse Interferon γ ,IFN-γ ELISA Kit  CSB-E04578m  96T            兔子γ干扰素(IFN-γ)ELISA Kit  rabbit Interferon γ ,IFN-γ ELISA Kit  CSB-E06925Rb  96T            鸡γ干扰素(IFN-γ)ELISA Kit  Chicken Interferon γ ,IFN-γ ELISA Kit  CSB-E08550Ch  96T            鹿γ干扰素(IFNG) ELISA kit  Deer interferon, gamma (IFNG) ELISA kit  CSB-EL011050DE  96T            猴γ干扰素(IFN-γ)ELISA Kit  Monkey Interferon γ ,IFN-γ ELISA Kit  CSB-E10049Mo  96T            Horse Interferon gamma(IFNG) ELISA kit  Horse Interferon gamma(IFNG) ELISA kit  CSB-EL011050HO  96T            Donkey Interferon gamma(IFNG) ELISA kit  Donkey Interferon gamma(IFNG) ELISA kit  CSB-EL011050DK  96T            犬γ干扰素(IFN-γ)ELISA Kit  Dog Interferon γ ,IFN-γ ELISA Kit  CSB-E11262c  96T            Cat Interferon gamma(IFNG) ELISA kit  Cat Interferon gamma(IFNG) ELISA kit  CSB-EL011050CA  96T           抗体  产品名称 货号 规格  Rabbit anti-human interferon, gamma polyclonal Antibody  CSB-PA011050GA01HU  150ul         蛋白  产品名称 货号 规格 蛋白来源  Recombinant Meleagris gallopavo Interferon gamma(IFNG)  1mg            Recombinant Coturnix coturnix japonica Interferon gamma(IFNG)  1mg            Recombinant Numida meleagris Interferon gamma(IFNG)  1mg            Recombinant Phasianus colchicus colchicus Interferon gamma(IFNG)  1mg            Recombinant Marmota monax Interferon gamma(IFNG)  1mg            Recombinant Mesocricetus auratus Interferon gamma(IFNG)  1mg            Recombinant Mustela putorius furo Interferon gamma(IFNG)  1mg            Recombinant Rat Interferon gamma(Ifng)  1mg            Recombinant Macaca nemestrina Interferon gamma(IFNG)  1mg            Recombinant Macaca fascicularis Interferon gamma(IFNG)  1mg            Recombinant Callithrix jacchus Interferon gamma(IFNG)  1mg            Recombinant Cercocebus atys Interferon gamma(IFNG)  1mg            Recombinant Cervus elaphus Interferon gamma(IFNG)  1mg            Recombinant Donkey Interferon gamma(IFNG)  1mg            Recombinant Mouse Interferon gamma(Ifng)  1mg            Recombinant Bovine Interferon gamma(IFNG)  1mg            Recombinant Human Interferon gamma(IFNG)  1mg            Recombinant Macaca mulatta (Rhesus macaque) Interferon gamma(IFNG)  1mg            Recombinant Chicken Interferon gamma(IFNG)  1mg            Recombinant Cat Interferon gamma(IFNG)  1mg            Recombinant Horse Interferon gamma(IFNG)  1mg            Recombinant Dog Interferon gamma(IFNG)  1mg            Recombinant Rabbit Interferon gamma(IFNG)  1mg            Recombinant Pig Interferon gamma(IFNG)  1mg            Recombinant Sheep Interferon gamma(IFNG)  1mg            Recombinant Goat Interferon gamma(IFNG)  1mg

 产品名称： 大鼠γ干扰素(IFN-γ)ELISA Kit    英文名称： Rat Interferon γ ,IFN-γ ELISA Kit    别名： IFG, IFI    货号： CSB-E04579r    价格： 3900    规格： 96T    种属： Rat    待测物名称： interferon, gamma    缩写： IFNG    样本类型： serum, plasma and tissue homogenates    检测范围： 0.625 pg/ml-40 pg/ml    灵敏度： 0.156 pg/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFNG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFNG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFNG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFNG bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Rat IFNG. No significant cross-reactivity or interference between Rat IFNG and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IFNG concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   相关产品：   产品名称 英文名称 货号 规格  山羊γ干扰素(IFN-γ)ELISA Kit  Goat Interferon γ ,IFN-γ ELISA Kit  CSB-E12966G  96T            仓鼠γ干扰素(IFNG)ELISA kit  Hamster interferon, gamma (IFNG) ELISA kit  CSB-EL011050HA  96T            猪γ干扰素(IFN-γ)ELISA Kit  Pig Interferon γ ,IFN-γ ELISA Kit  CSB-E06794p  96T            牛γ干扰素(IFN-γ)ELISA Kit  Bovine Interferon γ ,IFN-γ ELISA Kit  CSB-E09812b  96T            鸭γ干扰素(IFNG) ELISA kit  Duck interferon, gamma (IFNG) ELISA kit  CSB-EL011050DU  96T            鱼γ干扰素(IFN-γ)ELISA Kit  Fish Interferon γ ,IFN-γ ELISA Kit  CSB-E12878Fh  96T            绵羊γ干扰素(IFN-γ)ELISA Kit  Sheep Interferon γ ,IFN-γ ELISA Kit  CSB-E14018Sh  96T            人γ干扰素(IFN-γ)ELISA Kit  Human Interferon γ ,IFN-γ ELISA Kit  CSB-E04577h  96T            小鼠γ干扰素(IFN-γ)ELISA Kit  Mouse Interferon γ ,IFN-γ ELISA Kit  CSB-E04578m  96T            兔子γ干扰素(IFN-γ)ELISA Kit  rabbit Interferon γ ,IFN-γ ELISA Kit  CSB-E06925Rb  96T            鸡γ干扰素(IFN-γ)ELISA Kit  Chicken Interferon γ ,IFN-γ ELISA Kit  CSB-E08550Ch  96T            鹿γ干扰素(IFNG) ELISA kit  Deer interferon, gamma (IFNG) ELISA kit  CSB-EL011050DE  96T            猴γ干扰素(IFN-γ)ELISA Kit  Monkey Interferon γ ,IFN-γ ELISA Kit  CSB-E10049Mo  96T            Horse Interferon gamma(IFNG) ELISA kit  Horse Interferon gamma(IFNG) ELISA kit  CSB-EL011050HO  96T            Donkey Interferon gamma(IFNG) ELISA kit  Donkey Interferon gamma(IFNG) ELISA kit  CSB-EL011050DK  96T            犬γ干扰素(IFN-γ)ELISA Kit  Dog Interferon γ ,IFN-γ ELISA Kit  CSB-E11262c  96T            Cat Interferon gamma(IFNG) ELISA kit  Cat Interferon gamma(IFNG) ELISA kit  CSB-EL011050CA  96T           抗体  产品名称 货号 规格  Rabbit anti-human interferon, gamma polyclonal Antibody  CSB-PA011050GA01HU  150ul         蛋白  产品名称 货号 规格 蛋白来源  Recombinant Meleagris gallopavo Interferon gamma(IFNG)  1mg            Recombinant Coturnix coturnix japonica Interferon gamma(IFNG)  1mg            Recombinant Numida meleagris Interferon gamma(IFNG)  1mg            Recombinant Phasianus colchicus colchicus Interferon gamma(IFNG)  1mg            Recombinant Marmota monax Interferon gamma(IFNG)  1mg            Recombinant Mesocricetus auratus Interferon gamma(IFNG)  1mg            Recombinant Mustela putorius furo Interferon gamma(IFNG)  1mg            Recombinant Rat Interferon gamma(Ifng)  1mg            Recombinant Macaca nemestrina Interferon gamma(IFNG)  1mg            Recombinant Macaca fascicularis Interferon gamma(IFNG)  1mg            Recombinant Callithrix jacchus Interferon gamma(IFNG)  1mg            Recombinant Cercocebus atys Interferon gamma(IFNG)  1mg            Recombinant Cervus elaphus Interferon gamma(IFNG)  1mg            Recombinant Donkey Interferon gamma(IFNG)  1mg            Recombinant Mouse Interferon gamma(Ifng)  1mg            Recombinant Bovine Interferon gamma(IFNG)  1mg            Recombinant Human Interferon gamma(IFNG)  1mg            Recombinant Macaca mulatta (Rhesus macaque) Interferon gamma(IFNG)  1mg            Recombinant Chicken Interferon gamma(IFNG)  1mg            Recombinant Cat Interferon gamma(IFNG)  1mg            Recombinant Horse Interferon gamma(IFNG)  1mg            Recombinant Dog Interferon gamma(IFNG)  1mg            Recombinant Rabbit Interferon gamma(IFNG)  1mg            Recombinant Pig Interferon gamma(IFNG)  1mg            Recombinant Sheep Interferon gamma(IFNG)  1mg            Recombinant Goat Interferon gamma(IFNG)  1mg

 产品名称： 人Ⅰ型胶原(Col Ⅰ)ELISA Kit    英文名称： Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit    货号： CSB-E08082h    价格： 3600    规格： 96T    种属： Human    待测物名称： Collagen Type Ⅰ,Col Ⅰ    缩写： Col Ⅰ    样本类型： serum, urine, cell culture supernates, tissue homogenates    检测范围： 1.56 ng/ml-100 ng/ml    灵敏度： 0.39ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    相关产品：    产品名称 英文名称 货号 规格  小鼠Ⅰ型胶原(Col Ⅰ) ELISA Kit  Mouse Collagen Type Ⅰ,Col ⅠELISA Kit  CSB-E08083m  96T            小鼠Ⅱ型胶原(Col Ⅱ)ELISA Kit  Mouse Collagen Type Ⅱ,Col Ⅱ ELISA Kit  CSB-E08089m  96T            大鼠Ⅰ型前胶原羧基端肽(PⅠCP)ELISA Kit  Rat Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit  CSB-E08081r  96T            兔子Ⅰ型胶原(Col Ⅰ)ELISA Kit  rabbit Collagen Type Ⅰ,Col Ⅰ ELISA Kit  CSB-E06858Rb  96T            大鼠Ⅰ型胶原(Col Ⅰ)ELISA Kit  Rat Collagen Type Ⅰ,Col Ⅰ ELISA Kit  CSB-E08084r  96T            小鼠Ⅰ型前胶原羧基端肽(PⅠCP)ELISA Kit  Mouse Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit  CSB-E08080m  96T            大鼠维生素B6(VB6)ELISA Kit  Rat Vitamin B6,VB6 ELISA Kit  CSB-E08086r  96T            小鼠维生素B6(VB6)ELISA Kit  Mouse Vitamin B6,VB6 ELISA Kit  CSB-E08088m  96T            人维生素B6(VB6)ELISA Kit  Human Vitamin B6,VB6 ELISA Kit  CSB-E08085h  96T            人Ⅱ型胶原(Col Ⅱ)ELISA Kit  Human Collagen Type Ⅱ,Col Ⅱ ELISA Kit  CSB-E08087h  96T

 产品名称： 人Ⅱ型胶原(Col Ⅱ)ELISA Kit    英文名称： Human Collagen Type Ⅱ,Col Ⅱ ELISA Kit    货号： CSB-E08087h    价格： 3600    规格： 96T    种属： Human    待测物名称： Collagen Type Ⅱ,Col Ⅱ    缩写： Col Ⅱ    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    相关产品：    产品名称 英文名称 货号 规格  小鼠Ⅰ型胶原(Col Ⅰ) ELISA Kit  Mouse Collagen Type Ⅰ,Col ⅠELISA Kit  CSB-E08083m  96T            小鼠Ⅱ型胶原(Col Ⅱ)ELISA Kit  Mouse Collagen Type Ⅱ,Col Ⅱ ELISA Kit  CSB-E08089m  96T            人Ⅰ型胶原(Col Ⅰ)ELISA Kit  Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit  CSB-E08082h  96T            大鼠Ⅰ型前胶原羧基端肽(PⅠCP)ELISA Kit  Rat Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit  CSB-E08081r  96T            大鼠Ⅰ型胶原(Col Ⅰ)ELISA Kit  Rat Collagen Type Ⅰ,Col Ⅰ ELISA Kit  CSB-E08084r  96T            绵羊Ⅱ型胶原(Col Ⅱ)ELISA kit  Sheep collagen type Ⅱ(Col Ⅱ) ELISA kit  CSB-EQ027291SH  96T            小鼠Ⅰ型前胶原羧基端肽(PⅠCP)ELISA Kit  Mouse Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit  CSB-E08080m  96T            大鼠Ⅱ型胶原(Col Ⅱ)ELISA Kit  Rat Collagen Type Ⅱ,Col Ⅱ ELISA Kit  CSB-E08092r  96T            鸡Ⅱ型胶原(Col Ⅱ)ELISA kit  Chicken collagen type Ⅱ(Col Ⅱ) ELISA kit  CSB-EQ027291CH  96T            大鼠维生素B6(VB6)ELISA Kit  Rat Vitamin B6,VB6 ELISA Kit  CSB-E08086r  96T            小鼠维生素B6(VB6)ELISA Kit  Mouse Vitamin B6,VB6 ELISA Kit  CSB-E08088m  96T            人维生素B6(VB6)ELISA Kit  Human Vitamin B6,VB6 ELISA Kit  CSB-E08085h  96T            兔子Ⅱ型胶原(Col Ⅱ)ELISA Kit  rabbit Collagen Type Ⅱ,Col Ⅱ ELISA Kit  CSB-E06856Rb  96T

 产品名称： 人Ⅲ型胶原(Col Ⅲ)ELISA KIT    英文名称： Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT    货号： CSB-E04799h    价格： 3600    规格： 96T    种属： Human    待测物名称： Collagen Type Ⅲ,Col Ⅲ    缩写： Col Ⅲ    样本类型： serum, plasma, tissue homogenates    检测范围： 12.5 ng/ml-800 ng/ml    灵敏度： 3.12 ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    相关产品：    产品名称 英文名称 货号 规格  猪核因子-κB(NF-κB)ELISA Kit  Pig Nuclear factor-kappa B,NF-κB ELISA Kit  CSB-E13448p  96T            人可的松ELISA Kit  Human Cortisone ELISA Kit  CSB-E13444h  96T            人氧还原蛋白过氧化物酶4(PRDX4) ELISA Kit  Human Peroxiredoxin 4(PRDX4) ELISA Kit  CSB-E13442h  96T            猪Ⅲ型胶原(Col Ⅲ)ELISA Kit  Pig Collagen Type Ⅲ,Col Ⅲ ELISA Kit  CSB-E06776p  96T            Human Collagen, type V, alpha 1 (COL5A1)ELISA Kit  Human Collagen, type V, alpha 1 (COL5A1)ELISA Kit  CSB-E13447h  96T            大鼠Ⅲ型胶原(Col Ⅲ)ELISA Kit  Rat Collagen Type Ⅲ,Col Ⅲ ELISA KIT  CSB-E07924r  96T            牛生长激素 (GH) ELISA kit  Bovine growth hormone,GH ELISA Kit  CSB-E13443B  96T            Human collagen, type I, alpha 1 (COL1A1) ELISA kit  Human collagen, type I, alpha 1 (COL1A1) ELISA kit  CSB-E13445h  96T            Human Collagen, type III, alpha 1 (COL3A1)ELISA Kit  Human Collagen, type III, alpha 1 (COL3A1)ELISA Kit  CSB-E13446h  96T            小鼠Ⅲ型胶原(Col Ⅲ)ELISA KIT  Mouse Collagen Type Ⅲ,Col Ⅲ ELISA KIT  CSB-E07925m  96T            豚鼠黄体激素(LH)ELISA Kit  Guinea Pig Luteinizing Hormone,LH ELISA Kit  CSB-E13441Gp  96T            猪转录因子AP-1 ELISA kit  Pig Transcription Factor/Activator Protein 1 (AP-1) ELISA KIT  CSB-E13449p  96T            兔子Ⅲ型胶原(Col Ⅲ)ELISA Kit  rabbit Collagen Type Ⅲ,Col Ⅲ ELISA KIT  CSB-E06849Rb  96T            犬Ⅲ型胶原(Col Ⅲ)ELISA Kit  Canine Collagen Type Ⅲ (Col Ⅲ)ELISA Kit  CSB-E16374c  96T

 产品名称： 人Klotho ELISA Kit    英文名称： Human Klotho ELISA Kit    货号： CSB-E13235h    价格： 3600    规格： 96T    种属： Human    待测物名称： klotho    缩写： KL    样本类型： serum, plasma, tissue homogenates    检测范围： 0.156 ng/ml-10 ng/ml    灵敏度： 0.039 ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KL bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Human KL. No significant cross-reactivity or interference between Human KL and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the KL concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   相关产品：   产品名称 英文名称 货号 规格  小鼠Klotho ELISA Kit  Mouse Klotho ELISA Kit  CSB-E14362m  96T            大鼠 Klotho ELISA Kit  Rat Klotho ELISA Kit  CSB-E14958r  96T           蛋白   产品名称 货号 规格 蛋白来源  Recombinant Rat Klotho(Kl)  1mg            Recombinant Human Klotho(KL)  1mg            Recombinant Macaca fascicularis Klotho(KL)  1mg            Recombinant Mouse Klotho(Kl)  1mg

 产品名称： 大鼠β淀粉样蛋白1-42(Aβ1-42)ELISA Kit    英文名称： Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit    货号： CSB-E10786r    价格： 3900    规格： 96T    种属： Rat    待测物名称： amyloid beta peptide 1-42,Aβ1-42    缩写： Aβ1-42    样本类型： serum, plasma, tissue homogenates    检测范围： 0.9 pg/ml-60 pg/ml    灵敏度： 0.225 pg/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    相关产品：    产品名称 英文名称 货号 规格  小鼠β淀粉样蛋白1-42(Aβ1-42)ELISA Kit  Mouse amyloid beta peptide 1-42,Aβ1-42 ELISA Kit  CSB-E10787m  96T            人β淀粉样蛋白1-42(Aβ1-42)ELISA Kit  Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit 96T  CSB-E10684h  96T            猴β淀粉样蛋白1-42(Aβ1-42)ELISA Kit  Monkey amyloid beta peptide 1-42,Aβ1-42 ELISA Kit  CSB-E14398Mk  96T

产品名称： 猴子睾酮(T)ELISA Kit    英文名称： Monkey Testosterone,T ELISA Kit    货号： CSB-E16337Mk    规格： 96T    种属： Monkey    待测物名称： Testosterone(T)    缩写： T    样本类型： serum, plasma, cell culture supernates and tissue homogenates    检测范围： 0.08 ng/ml-20 ng/ml    灵敏度： 0.05ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  鸡睾酮(T)ELISA Kit  Chicken Testosterone (T) ELISA Kit  CSB-E12797C  96T            犬睾酮(T)ELISA Kit  Canine Testosterone (T) ELISA Kit  CSB-E06893c  96T            猫睾酮(T)ELISA kit  Cat testosterone (T) ELISA kit  CSB-E17329Fe  96T            人睾酮(T)ELISA Kit  Human Testosterone,T ELISA Kit  CSB-E05099h  96T            田鼠睾酮(T)ELISA Kit  Field mouse testosterone (T) ELISA kit  CSB-E15818Fm  96T            小鼠睾酮(T)ELISA Kit  Mouse Testosterone,T ELISA Kit  CSB-E05101m  96T            绵羊睾酮(T)ELISA kit  Sheep Testosterone (T) ELISA kit  CSB-E13171Sh  96T            鱼睾酮(T)ELISA Kit  Fish Testosterone(T) ELISA Kit  CSB-E17554Fh  96T            大鼠睾酮(T)ELISA Kit  Rat Testosterone,T ELISA Kit  CSB-E05100r  96T            蝌蚪睾酮(T)ELISA kit  Tadpole Testosterone (T) ELISA kit  CSB-E12735T  96T            大象睾酮(T)ELISA kit  Elephant Testosterone (T) ELISA kit  CSB-E13580El  96T            山羊睾酮(T)ELISA kit  Goat Testosterone (T) ELISA kit  CSB-E13630G  96T            鲸睾酮(T)ELISA Kit  Whale Testosterone(T) ELISA Kit  CSB-E15937Wh  96T            鲨鱼睾酮(T)ELISA Kit  Shark Testosterone(T) ELISA Kit  CSB-E15949Sk  96T            海狮睾酮(T)ELISA Kit  Sea Lion Testosterone(T) ELISA kit  CSB-E15943Sl  96T            兔子睾酮(T)ELISA Kit  rabbit Testosterone,T ELISA Kit  CSB-E06927Rb  96T            猪睾酮(T)ELISA Kit  Pig Testosterone,T ELISA Kit  CSB-E06796p  96T            Mouse Brachyury protein(T) ELISA kit  Mouse Brachyury protein(T) ELISA kit  CSB-EL023052MO  96T            蜥蜴睾酮(T) ELISA kit  Lizard Testosterone,T ELISA kit  CSB-E11996L  96T            Human Brachyury protein(T) ELISA kit  Human Brachyury protein(T) ELISA kit  CSB-EL023052HU  96T            马睾酮(T)ELISA Kit  Horse Testosterone(T) ELISA Kit  CSB-E13193Hs  96T            Dog Brachyury protein(T) ELISA kit  Dog Brachyury protein(T) ELISA kit  CSB-EL023052DO  96T            Chicken Brachyury protein(T) ELISA kit  Chicken Brachyury protein(T) ELISA kit  CSB-EL023052CH  96T            牛睾酮(T)ELISA Kit  Bovine Testosterone(T) ELISA Kit  CSB-E13194B  96T

 产品名称： 犬心肌肌钙蛋白Ⅰ(cTn-Ⅰ)ELISA kit Kit    英文名称： Canine Cardiac Troponin Ⅰ，cTn-Ⅰ ELISA kit    货号： CSB-E08772c    规格： 96T    种属： Dog    待测物名称： Cardiac Troponin Ⅰ(cTn-Ⅰ)    缩写： cTn-Ⅰ    样本类型： serum, plasma, tissue homogenates    检测范围： 125 pg/ml-8000 pg/ml    灵敏度： 31.25pg/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  猴子心肌钙蛋白I ELISA kit  Rhesus monkey cardiac troponin Ⅰ,cTn-ⅠELISA kit  CSB-EL024013RH  96T            大鼠纤溶抑制因子/凝血酶激活的纤溶抑制物(TAFI)ELISA Kit  Rat Thrombin activatable fibrinolysis inhibitor,TAFI ELISA Kit  CSB-E08779r  96T            大鼠心肌肌钙蛋白Ⅰ(cTn-Ⅰ)ELISA Kit  Rat Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit  CSB-E08594r  96T            大鼠血小板膜糖蛋白ⅡbⅢa(GP-ⅡbⅢa/CD41+CD61)ELISA Kit  Rat platelet membrane glycoprotein ⅡbⅢa,GP-ⅡbⅢa ELISA Kit  CSB-E08776r  96T            小鼠游离脂肪酸(FFA)ELISA Kit  Mouse free fatty acids,FFA ELISA Kit  CSB-E08771m  96T            人EJ抗体/抗甘氨酰tRNA合成酶抗体(EJ/GlyRS)ELISA Kit  Human anti-EJ-antibody，EJ/GlyRS ELISA Kit  CSB-E08773h  96T            猴子白三烯C4(LTC4)ELISA Kit  Monkey Leukotriene C4,LT-C4 ELISA Kit  CSB-E08774Mo  96T            人血小板膜糖蛋白ⅡbⅢa(GP-ⅡbⅢa/CD41+CD61)ELISA Kit  Human platelet membrane glycoprotein ⅡbⅢa,GP-ⅡbⅢa ELISA Kit  CSB-E08775h  96T            大鼠游离脂肪酸(FFA)ELISA Kit  Rat free fatty acids,FFA ELISA Kit  CSB-E08770r  96T            人纤溶抑制因子/凝血酶激活的纤溶抑制物(TAFI)ELISA Kit  Human Thrombin activatable fibrinolysis inhibitor,TAFI ELISA Kit  CSB-E08778h  96T

 产品名称： 小鼠粘蛋白5AC(MUC5AC)ELISA Kit    英文名称： Mouse Mucin-5 subtype AC,MUC5AC ELISA Kit    货号： CSB-E11040m    规格： 96T    种属： Mouse    待测物名称： Mucin-5 subtype AC,MUC5AC    缩写： MUC5AC    样本类型： serum, plasma, cell culture supernates and tissue homogenates    检测范围： 4.7 ng/ml-300 ng/ml    灵敏度： 1.17 ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  人粘蛋白5AC(MUC5AC) ELISA kit  Human mucin-5AC (MUC5AC) ELISA kit  CSB-E10109h  96T            猴载脂蛋白B100(Apo-B100)ELISA Kit  Monkey apolipoprotein B100 (Apo-B100) ELISA Kit  CSB-E11042Mo  96T            猴载脂蛋白A1(Apo-A1)ELISA Kit  Monkey apolipoprotein A1 (Apo-A1) ELISA Kit  CSB-E11041Mo  96T            大鼠粘蛋白/粘液素5AC(MUC5AC)ELISA Kit  Rat Mucin-5 subtype AC(MUC5AC)ELISA Kit  CSB-E16223r  96T            猪粘蛋白/粘液素5AC(MUC5AC)ELISA Kit  Pig mucin-5 subtype AC,MUC5AC ELISA Kit  CSB-E15069p  96T

 产品名称： 人结合珠蛋白/触珠蛋白(Hpt/HP)ELISA Kit    英文名称： Human Haptoglobin,Hpt/HP ELISA KIT    别名： BP, HP2-ALPHA-2, HPA1S    货号： CSB-E08584h    规格： 96T    种属： Human    待测物名称： haptoglobin    缩写： HP    样本类型： serum, plasma, urine    检测范围： 1.56 ng/ml-100ng/ml    灵敏度： 0.39ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HP bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Human HP. No significant cross-reactivity or interference between Human HP and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HP concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   相关产品：   产品名称 英文名称 货号 规格  绵羊结合珠蛋白/触珠蛋白(HP) ELISA kit  Sheep haptoglobin (HP) ELISA kit  CSB-EL010691SH  96T            鹿结合珠蛋白/触珠蛋白(HP) ELISA kit  Deer haptoglobin (HP) ELISA kit  CSB-EL010691DE  96T            Rabbit haptoglobin (HP) ELISA kit  Rabbit haptoglobin (HP) ELISA kit  CSB-EL010691RB  96T            大鼠结合珠蛋白/触珠蛋白(Hpt/HP)ELISA Kit  Rat Haptoglobin,Hpt/HP ELISA KIT  CSB-E08587r  96T            猪结合珠蛋白/触珠蛋白(Hpt/HP)ELISA Kit  Pig Haptoglobin,Hpt/HP ELISA Kit  CSB-E13424p  96T            小鼠结合珠蛋白/触珠蛋白(Hpt/HP)ELISA Kit  Mouse Haptoglobin,Hpt/HP ELISA KIT  CSB-E08586m  96T            Dog haptoglobin (HP) ELISA kit  Dog haptoglobin (HP) ELISA kit  CSB-EL010691DO  96T            牛结合珠蛋白/触珠蛋白(Hpt/HP)ELISA Kit  Bovine Haptoglobin,Hpt/HP ELISA KIT  CSB-E08585b  96T           抗体  产品名称 货号 规格  Rabbit anti-human haptoglobin polyclonal Antibody  CSB-PA010691GA01HU  150ul         蛋白  产品名称 货号 规格 蛋白来源  Recombinant Rat Haptoglobin(Hp)  CSB-EP010691RA  1mg  E.coli            Recombinant Rat Haptoglobin(Hp)  CSB-YP010691RA  1mg  Yeast            Recombinant Ateles geoffroyi Haptoglobin(HP)  CSB-EP010691AVF  1mg  E.coli            Recombinant Ateles geoffroyi Haptoglobin(HP)  CSB-YP010691AVF  1mg  Yeast            Recombinant Mesocricetus auratus Haptoglobin(HP)  CSB-EP010691MRG  1mg  E.coli            Recombinant Mesocricetus auratus Haptoglobin(HP)  CSB-YP010691MRG  1mg  Yeast            Recombinant Capra ibex Haptoglobin(HP)  CSB-EP010691CZZ  1mg  E.coli            Recombinant Capra ibex Haptoglobin(HP)  CSB-YP010691CZZ  1mg  Yeast            Recombinant Cervus elaphus Haptoglobin(HP)  CSB-EP010691DRN  1mg  E.coli            Recombinant Cervus elaphus Haptoglobin(HP)  CSB-YP010691DRN  1mg  Yeast            Recombinant Human Haptoglobin(HP)  CSB-EP010691HU  1mg  E.coli            Recombinant Human Haptoglobin(HP)  CSB-YP010691HU  1mg  Yeast            Recombinant Rabbit Haptoglobin(HP)  CSB-EP010691RB  1mg  E.coli            Recombinant Rabbit Haptoglobin(HP)  CSB-YP010691RB  1mg  Yeast            Recombinant Dog Haptoglobin(HP)  CSB-EP010691DO  1mg  E.coli            Recombinant Dog Haptoglobin(HP)  CSB-YP010691DO  1mg  Yeast

产品名称： 人白介素6(IL-6)ELISA KIT    英文名称： Human Interleukin 6,IL-6 ELISA KIT    别名： BSF2, HGF, HSF, IFNB2, IL-6    货号： CSB-E04638h    规格： 96T    种属： Human    待测物名称： interleukin 6     缩写： IL6    蛋白功能1： Immunity    蛋白功能3： Acute phase    样本类型： serum, plasma, cell culture supernates, urine, tissue homogenates    检测范围： 7.8 pg/ml-500 pg/ml    灵敏度： 1.95 pg/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Human IL6. No significant cross-reactivity or interference between Human IL6 and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL6 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   相关产品： 产品名称 英文名称 货号 规格  猪白介素6(IL-6)ELISA Kit  Pig Interleukin 6,IL-6 ELISA Kit  CSB-E06786p  96T            牛白介素6(IL-6)ELISA Kit  Bovine Interleukin 6,IL-6 ELISA Kit  CSB-E12899B  96T            人超敏白介素6(hs-IL6) ELISA kit  Human high sensitivity interleukin 6 (hs-IL6) ELISA kit  CSB-EQ011664HU  96T            绵羊白介素6(IL-6)ELISA Kit  Sheep Interleukin 6,IL-6 ELISA Kit  CSB-E10116Sh  96T            大鼠白介素6(IL-6)ELISA KIT  Rat Interleukin 6,IL-6 ELISA KIT  CSB-E04640r  96T            小鼠白介素6(IL-6)ELISA Kit  Mouse Interleukin 6,IL-6 ELISA KIT  CSB-E04639m  96T            猫白介素6(IL-6)ELISA Kit  Cat Interleukin 6(IL-6) ELISA Kit  CSB-E14046Fe  96T            鸡白介素6(IL-6)ELISA Kit  Chicken Interleukin 6,IL-6 ELISA Kit  CSB-E08549Ch  96T            马白介素6(IL-6)ELISA Kit  Horse Interleukin 6(IL-6)ELISA Kit  CSB-E16634Hs  96T            兔子白介素6(IL-6)ELISA Kit  rabbit Interleukin 6,IL-6 ELISA Kit  CSB-E06903Rb  96T            猴白介素6(IL-6)ELISA Kit  Monkey Interleukin 6,IL-6 ELISA Kit  CSB-E10050Mo  96T            山羊白介素6(IL-6)ELISA Kit  Goat Interleukin 6,IL-6 ELISA Kit  CSB-E14360G  96T            犬白介素6(IL-6)ELISA Kit  Dog Interleukin 6,IL-6 ELISA Kit  CSB-E11260c  96T           蛋白  产品名称 货号 规格 蛋白来源  Recombinant Marmota monax Interleukin-6(IL6)  CSB-EP011664MQG  1mg  E.coli            Recombinant Marmota monax Interleukin-6(IL6)  CSB-YP011664MQG  1mg  Yeast            Recombinant Sparus aurata Interleukin-6(il6)  CSB-EP011664FJX  1mg  E.coli            Recombinant Sparus aurata Interleukin-6(il6)  CSB-YP011664FJX  1mg  Yeast            Recombinant Mustela putorius furo Interleukin-6(IL6)  CSB-EP011664MOH  1mg  E.coli            Recombinant Mustela putorius furo Interleukin-6(IL6)  CSB-YP011664MOH  1mg  Yeast            Recombinant Cercocebus atys Interleukin-6(IL6)  CSB-EP011664DRI  1mg  E.coli            Recombinant Cercocebus atys Interleukin-6(IL6)  CSB-YP011664DRI  1mg  Yeast            Recombinant Mustela vison Interleukin-6(IL6)  CSB-EP011664MVE  1mg  E.coli            Recombinant Mustela vison Interleukin-6(IL6)  CSB-YP011664MVE  1mg  Yeast            Recombinant Rat Interleukin-6(Il6)  CSB-EP011664RA  1mg  E.coli            Recombinant Rat Interleukin-6(Il6)  CSB-YP011664RA  1mg  Yeast            Recombinant Macaca fascicularis Interleukin-6(IL6)  CSB-EP011664MOV  1mg  E.coli            Recombinant Macaca fascicularis Interleukin-6(IL6)  CSB-YP011664MOV  1mg  Yeast            Recombinant Mouse Interleukin-6(Il6)  CSB-EP011664MO  1mg  E.coli            Recombinant Mouse Interleukin-6(Il6)  CSB-YP011664MO  1mg  Yeast            Recombinant Human Interleukin-6(IL6)  CSB-EP011664HU  1mg  E.coli            Recombinant Human Interleukin-6(IL6)  CSB-YP011664HU  1mg  Yeast            Recombinant Macaca mulatta (Rhesus macaque) Interleukin-6(IL6)  CSB-EP011664  1mg  E.coli            Recombinant Macaca mulatta (Rhesus macaque) Interleukin-6(IL6)  CSB-YP011664  1mg  Yeast            Recombinant Cat Interleukin-6(IL6)  CSB-EP011664CA  1mg  E.coli            Recombinant Cat Interleukin-6(IL6)  CSB-YP011664CA  1mg  Yeast            Recombinant Sheep Interleukin-6(IL6)  CSB-EP011664SH  1mg  E.coli            Recombinant Sheep Interleukin-6(IL6)  CSB-YP011664SH  1mg  Yeast            Recombinant Pig Interleukin-6(IL6)  CSB-EP011664PI  1mg  E.coli            Recombinant Pig Interleukin-6(IL6)  CSB-YP011664PI  1mg  Yeast            Recombinant Dog Interleukin-6(IL6)  CSB-EP011664DO  1mg  E.coli            Recombinant Dog Interleukin-6(IL6)  CSB-YP011664DO  1mg  Yeast            Recombinant Bovine Interleukin-6(IL6)  CSB-EP011664BO  1mg  E.coli            Recombinant Bovine Interleukin-6(IL6)  CSB-YP011664BO  1mg  Yeast

上海希美提供食品安全及药物残留ELISA KIT CUSABIO提供最全、最新食品安全及药物残留ELISA KIT 欢迎广大老师来电咨询、订购！！！                产品名称                                                                     英文名称                                       磺胺类药物残留(SAs)ELISA kit                        Sulfonamides (SAs) residue ELISA kit           庆大霉素(GEN)ELISA kit                                  Gentamicin (GEN) ELISA kit           克伦特罗(CL)ELISA kit                                     Clenbuterol (CL) ELISA kit           氯霉素(CM)ELISA kit                                        Chloramphenicol (CM) ELISA kit          无色孔雀石绿(LMG)ELSIA kit                          Leuco malachite green (LMG) ELSIA kit           磺胺甲恶唑(SMZ/SMX)ELSIA kit                     Sulfamethoxazole (SMZ/SMX) ELSIA kit           赭曲霉素A(OCH-A)ELISA kit                           Ochratoxin-A (OCH-A) ELISA kit    总黄曲霉毒素(AFT)ELISA Kit                           Total Aflatoxin,AFT ELISA Kit   黄曲霉毒素B1(AFB1)ELISA kit                          Aflatoxin B1(AFB1)ELISA kit β-兴奋剂(BAs)ELISA kit                                      Beta-agonists (BAs) ELISA kit           莱克多巴胺(RA)ELISA kit                                  Ractopamine (RA) ELISA kit           磺胺嘧啶(SD)ELISA kit                                       Sulfadiazine (SD) ELISA kit          诺恩沙星(ER)ELISA kit                                       Enrofloxacin (ER) ELISA kit             呋喃西林（SEM）ELISA检测试剂盒               Nitrofurazone(SEM) ELISA kit             四环素(TET)快速检测试剂盒                             Tetracycline (TET) ELISA kit            链霉素(SM) 快速检测试剂盒                              Streptomycin (SM) ELISA kit    磺胺噻唑(ST)快速检测试剂盒                              Sulfathiazole (ST) ELISA kit          黄曲霉毒素M1(AFM1)ELISA Kit  Aflatoxin M1(AFM1)ELISA kit          四环素(TET)快速检测试剂盒  Tetracycline (TET) ELISA kit         青霉素G(BP)快速检测试剂盒  Penicillin G(benzyl penicillin) ELISA kit