全基因合成服务上海希美以精湛的技术为您提供高质量快速的全基因合成服务。无论什么种属的基因，您只需提供需要合成的序列信息，我们免费为您进行密码子优化服务（Free codon optimization），使合成的基因能最大程度提高蛋白表达效果。操作程序1.软件分析您需合成的目的序列，检查目的基因中的重复序列及特殊结构，根据序列具体情况设计合成方案。2.进行单链Oligo DNA合成、DNA片段拼接等工作，免费将目的基因克隆于PET28a/PGEX-6P-1等载体中，测序分析DNA序列的准确性。3. 修复错误位点，完整全长基因合成。4.提供实验结果：测序结果；测序图谱；含有合成基因的质粒及该质粒的菌液等。

RT-PCR服务RT-PCR检测细胞或组织中基因表达变化，并以beta-actin或GAPDH等看家基因作为内参照，对目的基因的表达量进行半定量检测。与其他RNA分析技术如，Northern印迹、RNase保护分析、原位杂交及S1核酸酶等相比，RT-PCR具有更灵敏、便捷、价廉、易于操作等多重优势，已成为初步检测基因转录水平差异变化的首选实验方法之一，广泛应用于细胞基因表达、RNA病毒含量测定和克隆特定基因的cDNA序列等诸多方面。项目内容引物设计合成RNA提取逆转录合成cDNA第一链扩增目的基因琼脂糖凝胶电泳检测

杆状病毒-昆虫细胞表达与纯化杆状病毒表达系统(BEVS)属于真核表达系统，安全性高，由于有较大的基因组所以能够容纳较大的外源基因，得到的重组病毒易于筛选，并且具有完备的翻译后加工修饰系统和高效表达外源基因的能力。该系统由转移载体、杆状病毒载体和宿主细胞组成。该系统采用一个或多个杆状病毒的超强启动子，将外源目的基因插入到启动子后，获得重组病毒，这种重组病毒在昆虫细胞复制的同时，使外源基因得到高效表达。杆状病毒表达系统与其他表达系统相比，具有以下的优势：(1)安全性：杆状病毒具有严格的种属特异性，不感染脊椎动物和植物，相对于其他病毒载体，如痘病毒、腺病 毒载体等，有较高的安全性。(2)易于扩增：杆状病毒可以大规模生产具有生物学活性的重组蛋白产品。(3)外源基因表达效率高：与其他真核细胞表达系统相比，杆状病毒系统可以从受感染的晚期细胞中高效地表达外源蛋白。(4)表达产物具有较高活性：杆状病毒将会在昆虫宿主细胞中大量扩增，而且产生的重组蛋白会进行类似哺乳动物细胞中的翻译后修饰，表达产物具有很强的生物活性。昆虫细胞表达系统服务项目重组供体质粒构建重组杆状病毒载体构建表达条件优化蛋白表达与纯化利用交配辅助遗传整合克隆(MAGIC)系统，只需要简单的细菌混合，就可以快速构建重组杆状病毒载体，能够大大的节约时间，精力和花费； 可提供详细表达纯化条件，实验数据以及高滴度的病毒毒株

天然蛋白纯化过程复杂，通常包括样品提取、澄清、初纯化和精纯化等步骤。层析是蛋白纯化的主要手段，主要有亲和、离子交换、分子筛、疏水层析等方法，不同蛋白需根据其性质，选择不同的纯化策略。如果您有天然蛋白纯化技术服务的需求，请直接与我们联系，我们将第一时间与您探讨实验方案，并提供初步报价。服务项目   服务内容    天然蛋白纯化   根据目的蛋白性质，相关文献报道，制备亲和层析柱，综合利用亲和、离子交换、分子筛、疏水层析等方法对天然蛋白进行纯化   

酵母表达纯化服务    酵母蛋白表达系统是一种最经济高效的真核蛋白表达系统，可以成功实现胞内表达或是分泌表达，且酵母表达系统放大培养基相对廉价，培养条件要求不高，适宜工业放大。    哺乳动物细胞表达系统能够表达出接近天然蛋白的高质量蛋白，但是表达量低，培养基成本过高及培养条件相对苛刻，限制了哺乳动物细胞表达系统用于制备高质量蛋白原料的推广应用。酵母蛋白表达系统和哺乳动物细胞表达系统一样，作为真核表达系统能很好整合哺乳动物细胞表达系统的优点。能够对所表达蛋白进行例如糖基化、酰基化、脂基化、磷酸基化等保证蛋白天然构象的修饰。用于制备非常接近天然蛋白的具有高附加值的蛋白原料，事实证明我们的酵母蛋白表达系统服务经客户回馈所制备的蛋白原料经下游制备的单抗均具有很好的识别天然蛋白的优良特性。我们高效的种子筛选工作能使您的订单在最短的时间内获得尽可能的高产。     酵母表达系统由多年从事酵母表达操作的实验人员组成，在酵母表达平台有很多成功的案例，我们采用自行改造的高效分泌载体：Ppic9k、Ppic9k高效融合载体、PPICZa、PGAPZa、PPaSUMO3，宿主：GS115、KM71、X33的正交组合。另外我们采用PINK系统优化分泌表达信号肽。胞内表达我们采用自行改造的载体Ppic3.5k载体和宿主GS115、KM71、X33的组合，在最大程度上实现表达。

大肠杆菌表达纯化服务  全方位的重组蛋白生产服务，在表达包括可溶性蛋白、包涵体、融合蛋白等方面，拥有丰富的经验和专业技术。★His、GST、MBP等标签多种表达方式同时进行，确保获得最优质的蛋白，仅收取一份费用。★保证蛋白为普通缓冲可溶★续订蛋白可享受3-5折优惠★纯化的蛋白经过ELISA，Western-blot，或者其它生物活性验证。★可提供详细表达纯化条件，实验数据以及菌株服务项目基因合成表达载体构建表达条件优化原核表达蛋白纯化标签切除实验数据、表达菌株

上海希美专业从事抗体生产和定制服务，在鼠单克隆抗体开发方面有着自己丰富和独到的经验。近年来，我们不断优化单克隆抗体实验系统，从免疫、杂交瘤细胞融合到阳性杂交瘤细胞株筛选我们一直在深入改进，极大地提高了融合率和阳性率。在鼠单克隆抗体开发过程中，无论是您提供抗原，还是我们根据您提供的抗原信息来制备小分子、多肽或者蛋白抗原，我们都将会在最短时间内提供给您最高质量的单克隆抗体。对于难以制备的鼠单克隆抗体，我们有足够的经验和专业技术，能将鼠抗体基因克隆出来，以各种蛋白表达平台制备出来具有活性和功能的基因工程抗体。同时在单克隆抗体抗体纯化方面，我们也有着专业的技术，可以纯化包括IgG1、IgG2a、IgG2b以及 IgM 等鼠抗体种类。凭借我公司专业、综合的研发团队，无论您的单抗是用于科研的检测实验，还是用于诊断试剂的开发，或是用于抗体药物的前期研究，我们都将会结合我们多年的研发经验通过与您保持良好的沟通和我们对项目本身的深入的分析，以期从实验的设计早期开始最大程度的保证获得最适合您实验的单克隆抗体。最终提供：2-3 株杂交瘤细胞株和提供10ml腹水纯化的抗体，100ul腹水原液，1ml细胞上清，并给出相应的鉴定报告(包括以下内容：小鼠血清效价，杂交瘤的构建(融合率、阳性率)、杂交瘤的克隆化、杂交瘤培养上清液的抗体特异性检测，以及杂交瘤诱生腹水的ELISA 效价检测)。最终保证：提供的单抗腹水或者杂交瘤细胞株的上清经ELISA 检测与顾客方提供的抗原样品有特异性结合；提供的单抗腹水或者杂交瘤细胞株的上清经Western blot检测结果呈阳性。服务名称小鼠单克隆抗体制备服务(客户提供抗原)大鼠单克隆抗体制备服务小鼠快速单克隆抗体制备服务大鼠快速单克隆抗体制备服务抗独特型单克隆抗体制备服务（小鼠）修饰化单克隆抗体制备服务无血清培养基制备单克隆抗体3、技术优势①最新型服务套餐：抗体制备、抗体纯化、抗体筛选一体化服务；可提供多样的纯化方法，辛酸硫酸铵、Protein A/G 、抗原亲和层析等；抗体筛选为您节省时间，我们可根据客户需求筛选抗体对及天然样本识别的验证，让客户无后顾之忧。② 多样化定制服务：可提供小鼠及大鼠单克隆抗体服务平台、采用半固体模式快速制备单克隆抗体服务平台、无血清培养基单克隆抗体制备服务平台，客户可根据需求灵活选择。③ 抗原及免疫种类多样性：华美拥有强大的蛋白服务平台，客户可选择华美提供抗原，有多种表达系统供您选择，如哺乳蛋白更接近天然构象，可使您的抗体质量更高；另外我们每个项目至少免疫5只小鼠/大鼠，采用多种免疫途径，多管齐下，为得到更优质的单抗提供了保证。④大规模生产服务：可提供最专业的单克隆抗体大规模生产服务，满足诊断级大量生产的需求。⑤ 高质量：专业从事抗体生产和定制服务，以抗体平台为核心建立了一系列相关的技术平台和动物养殖基地。我们提供的单抗产品由常年从事抗体生产的专业人员完成，技术路线成熟，产品质量稳定可靠、灵敏度极高，至今为止本平台已经成功制备多种诊断试剂级别的单克隆抗体对原料，深受下游客户的好评。三.服务实例与全球多家知名科研试剂公司、诊断试剂公司、生物制药公司紧密合作，我们不断开发出能够符合客户特定应用要求的单克隆抗体，如应用于ELISA、IHC、IP、western blot等技术平台的科研抗体；可为客户开发出适合其特定应用要求的单克隆抗体配对，使之能够应用于ELISA、胶体金试纸条、化学发光试剂或免疫磁珠等平台的诊断抗体；也可为客户开发出高灵敏度的小分子、多肽类单克隆抗体；专业、高效、经济是我们对客户的郑重承诺。1.诊断级单克隆抗体定制①我公司为客户研发的某单克隆抗体共筛选到4株不同表位，均能两两配对；并且成功应用于ELISA、胶体金、胶乳免疫增强比浊法试剂盒的开发图1.1.某单抗胶乳增强免疫比浊法稳定性测试（线性及稳定性均较好）图1.2.某单抗胶乳增强免疫比浊法临床对比结果②我公司为客户研发某单克隆抗体对，经WB、ELISA、IHC等方法验证均能识别天然样本。

多克隆抗体服务多克隆抗体制备服务多克隆抗体存在于免疫动物的血清中，可通过直接分离血清获得。由于具有可识别多个表位、可引起凝集反应、沉淀反应等优点，被广泛应用于免疫学诊断领域。比如经常用到的Western blotting实验中的酶标二抗和ELISA实验中的包被抗体。而且制备费用也较单克隆抗体低。我们专业从事抗体生产和定制服务，您提供抗原或者我们帮您设计并制备抗原，我们提供给您满意的包括山羊、兔、小鼠、大鼠、豚鼠和鸡等多物种的抗体产品。我们承诺上海希美的多克隆抗体服务ELISA效价达到1：10,000以上。对于任何宿主、任何蛋白抗原（纯度>85%）的多克隆抗体服务保证Western blot检测结果呈阳性。兔      兔抗血清制备    山羊    山羊抗血清制备 小鼠    小鼠抗血清制备    大鼠    大鼠抗血清制备    豚鼠    豚鼠抗血清制备    鸡    鸡抗血清制备    客户须知1 您所提供制备抗体的抗原，要求纯度>85%，蛋白要求分子量大于10kDa2 客户提供的抗原的量由免疫动物的数量和种类所决定。可溶性蛋白，浓度最好在1–5mg/ml之间，最小不得低于0.5mg/ml。3 客户提供的抗原中不应含有叠氮钠、咪唑、尿素或其它有毒有害刺激性大的物质。甘油含量应低于20%。4 客户应对所提供的材料及信息负责，如因客户提供的材料及信息不准确而引起的实验延误或经济损失全部由客户承担。   

抗体纯化我公司可为客户提供兔、羊、豚鼠、鸡卵等种属来源的多抗以及小鼠单抗纯化技术服务。我们主要采用硫酸铵沉淀、Protein A/G、MEP、抗原亲和层析等常规方法，也可用离子交换、疏水、金属螯合层析等方法完成。我们还可以为客户提供抗体Fab片段制备服务。抗体纯度、活性的高低将直接影响试验的成败，我们将根据客户的特定用途选择最佳纯化方法，为客户解决体纯化的问题。服务项目服务内容价格服务周期抗体纯化    硫酸铵沉淀法纯化抗体    200.00/10ml腹水、细胞上清、抗血清    1-5天    ProteinA/G法纯化抗体    300.00/10ml腹水、细胞上清、抗血清    1-5天    MEP法纯化抗体    300.00/10ml腹水、细胞上清、抗血清    1-5天    亲和柱制备、抗原亲和纯化法纯化抗体    亲和柱制备：500.00/ml柱料纯化：300.00/10ml腹水、细胞上清、抗血清    3-10天    其他方法纯化抗体    300.00/10ml腹水、细胞上清、抗血清    3-10天    Fab片段制备    固定化木瓜蛋白酶消化、Fab片段纯化    500.00/mg抗体    3-5天    

抗体标记服务免疫标记技术是将已知抗体或抗原标记上一些既易测定又具有高度敏感性的物质，通过检测标记物的增强放大效应来显示反应系统中抗原抗体反应的情况，从而间接地测出被检抗原或抗体的存在与否或量的多少。抗体标记主要是用于抗原的定位分析，在某些情况下，也可以对混杂有大量其他分子的样本中的抗原进行定量检测。由于抗体与其相应抗原具有很高的亲和力，因此带有易识别标记物的抗体可以定位分析抗原，并且是一种理想的快速价廉的定量测定方法。抗体标记服务内容：酶（HRP）标记：酶标记抗体是将酶与特异性抗体经适当方法连接而成，其应用范围非常广泛，结果即时可见、敏感性高。荧光素(FITC)标记：荧光色素是最常用于标记抗体的标记物之一。荧光素经恰当的激发可发光，从而得知抗体的定位和待测抗原的分布情况，主要用于细胞分选或高分辨率免疫染色，是一种非常好的亚细胞水平精确定位的方法。生物素(Biotin)标记：生物素标记反应简单、温和且很少抑制抗体活性，将生物素与抗体共价结合是一种非常简便、直接的标记方法。服务项目材料要求标记量价格工作日交付结果应用范围效价HRP标记    浓度1mg/ml以上，纯度90%以上磷酸盐缓冲液保存的抗原或抗体    0.1-0.5mg    ￥400/100ug    3-5天    100ug/160ul(不加甘油)    ELISA    1：1000-5000    0.5-1mg    ￥350/100ug    1mg以上    ￥300/100ug    100ug/320ul(加甘油)    FITC标记    1-5mg    ￥600/500ug    3-5天    500ug/1ml(不加甘油)    ELISA    1：1000-5000    5-10mg    ￥550/500ug    5-7天    10mg以上    ￥500/500ug    7-10天    500ug/2ml(加甘油)    Biotin标记    1-5mg    ￥600/500ug    3-5天    500ug/1ml(不加甘油)    ELISA    1：1000-5000    

样本代测服务上海希美经过不断的实验优化和改进，积累了大量的经验，拥有专业的酶联研发团队。利用专业的酶联免疫技术自主研发的ELISA试剂盒，能对血清及其它样本定量检测抗原，定性检测特异性抗体。优质的试剂，先进的仪器和正确的操作是保证ELISA检测结果准确可靠的必要条件。ELISA检测的方便性、稳定性、重复性和可靠性方面都具有很大的优势。ELISA检测技术服务内容： 1、双抗体夹心法检测抗原 2、间接法检测抗体 3、为客户提供各种ELISA技术进行样本检测。服务项目服务类型客户提供材料及要求代测费交货时间提供结果抗原鉴定    客户自备试剂盒    可溶性抗原或由本公司进行原核表达（另外收费）    400元/次    5-10个工作日（本公司在收到客户提供的材料后会在第二个工作日起开始检测工作）    检测试验报告（包括曲线图）    本公司订购试剂盒    200元/次    抗体鉴定    客户自备试剂盒    一抗    400元/次    本公司订购试剂盒    200元/次    样本检测    客户自备试剂盒    培养上清，人和动物的血清，血浆等。 -20℃ 保存，避免反复冻融。    800元/次    本公司订购试剂盒    400元/次    注意事项：一、服务承诺：凡购买本公司目录任何一种酶联免疫检测试剂盒，您只需将需要检测的动物（Human, Rat, Mouse, Rabbit, Monkey, Pig……）种类和检测指标（白介素类、激素类）及标本数量（48T/96T）通知公司业务员即可。在接到客户标本当日起，现货产品二周内将检测报告交到客户手中！欢迎临床医学界和各科研单位在各种项目上与我们公司开展不同层次的密切合作，以双赢求发展，共同进步，为中国检测事业的发展积累经验。二、样本要求在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。代测放免标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。血清：室温血液自然凝固10-20分钟后，离心20分钟左右（2000-3000转/分）。收集上清。如有沉淀形成，应再次离心。血浆：应根据试剂盒的要求选择EDTA、柠檬酸钠或肝素作为抗凝剂，加入10%（v/v）抗凝剂(0.1M柠檬酸钠或1%heparin 或2.0%EDTA.Na2)混合10-20分钟后，离心20分钟左右（2000-3000转/分）。仔细收集上清。如有沉淀形成，应再次离心。尿液、胸腹水、脑脊液：用无菌管收集。离心20分钟左右（2000-3000转/分）。仔细收集上清。如有沉淀形成，应再次离心。细胞培养上清：检测分泌性的成份时，用无菌管收集。离心20分钟左右（2000-3000转/分）。仔细收集上清。检测细胞内的成份时，用PBS（PH7.0-7.4）稀释细胞悬液，细胞浓度达到100万/ml左右。通过反复冻融，以使细胞破坏并放出细胞内成份。离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。组织标本：切割标本后，称取重量。加入一定量的PBS，缓冲液中可加入1μg/L蛋白酶抑制剂或50U/ml的Aprotinin（抑肽酶）。用手工或匀浆器将标本匀浆充分。离心20分钟左右（2000-3000转/分）。仔细收集上清置于-20度或-70度保存，如有必要，可以将样品浓缩干燥。分装后一份待检测，其余冷冻备用。三、寄标本时需注明以下情况：1、标本编号；2、所测项目；3、是否做复孔；3、联系方式；4、实验后标本是否寄回。客户须知：客户应对所提供的材料及信息负责，如因客户提供的材料及信息不准确而引起的实验延误或经济损失由客户承担。

产品名称:鸡β防御素1(DEFB1)ELISA kit英文名称:Chicken Beta-defensin 1(DEFB1)ELISA kit货号:CSB-EL006662CH 别名: BD1, DEFB-1, DEFB101, HBD1, MGC51822, beta-defensin-1 规格: 96T 产品类型:ELISA Kit价格: 4200 种属: Chicken 其他种属:Bovine Goat Human Monkey Mouse Pig Rat Sheep 待测物名称: defensin, beta 1 缩写: DEFB1 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm

产品名称:绵羊β-防御素1(DEFB1)ELISA kit英文名称:Sheep Beta-defensin 1(DEFB1) ELISA kit货号:CSB-EL006662SH 别名: BD1, DEFB-1, DEFB101, HBD1, MGC51822, beta-defensin-1 规格: 96T 产品类型:ELISA Kit价格: 4200 种属: Sheep 其他种属:Bovine Goat Human Monkey Mouse Pig Rat Chicken 待测物名称: defensin, beta 1 缩写: DEFB1 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm

产品名称:大鼠β-防御素1(DEFB1)ELISA kit英文名称:Rat Beta-defensin 1(DEFB1)ELISA kit货号:CSB-EL006662RA 别名: BD1, DEFB-1, DEFB101, HBD1, MGC51822, beta-defensin-1 规格: 96T 产品类型:ELISA Kit价格: 3600 种属: Rat 其他种属:Bovine Goat Human Monkey Mouse Pig Sheep Chicken 待测物名称: defensin, beta 1 缩写: DEFB1 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm

产品名称:猪β-防御素1(DEFB1)ELISA kit英文名称:Pig Beta-defensin 1(DEFB1)ELISA kit货号:CSB-EL006662PI 别名: BD1, DEFB-1, DEFB101, HBD1, MGC51822, beta-defensin-1 规格: 96T 产品类型:ELISA Kit价格: 4200 种属: Pig 其他种属:Bovine Goat Human Monkey Mouse Rat Sheep Chicken 待测物名称: defensin, beta 1 缩写: DEFB1 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm

产品名称:小鼠β-防御素1 ELISA Kit英文名称:Mouse β-defensins 1 ELISA Kit货号:CSB-E14188m 别名: BD1, DEFB-1, DEFB101, HBD1, MGC51822, beta-defensin-1 规格: 96T 产品类型:ELISA Kit价格: 3600 种属: Mouse 其他种属:Bovine Goat Human Monkey Pig Rat Sheep Chicken 待测物名称: defensin, beta 1 缩写: DEFB1 检测范围: 15.6 pg/ml-1000 pg/ml 灵敏度: 3.9 pg/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm

产品名称:猴子β-防御素1(DEFB1)ELISA kit英文名称:Monkey Beta-defensin 1(DEFB1)ELISA kit货号:CSB-EL006662RH 别名: BD1, DEFB-1, DEFB101, HBD1, MGC51822, beta-defensin-1 规格: 96T 产品类型:ELISA Kit价格: 4200 种属: Monkey 其他种属:Bovine Goat Human Mouse Pig Rat Sheep Chicken 待测物名称: defensin, beta 1 缩写: DEFB1 检测范围: 78 pg/ml-5000 pg/ml 灵敏度: 19.5 pg/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm

产品名称:山羊β-防御素1(DEFB1)(DEFB1)ELISA kit英文名称:Goat Beta-defensin 1(DEFB1) ELISA kit货号:CSB-EL006662GO 别名: BD1, DEFB-1, DEFB101, HBD1, MGC51822, beta-defensin-1 规格: 96T 产品类型:ELISA Kit价格: 4200 种属: Goat 其他种属:Bovine Human Monkey Mouse Pig Rat Sheep Chicken 待测物名称: defensin, beta 1 缩写: DEFB1 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm

产品名称:牛β-防御素1(DEFB1)ELISA kit英文名称:Bovine Beta-defensin 1(DEFB1) ELISA kit货号:CSB-E17795B 别名: BD1, DEFB-1, DEFB101, HBD1, MGC51822, beta-defensin-1 规格: 96T 产品类型:ELISA Kit价格: 4200 种属: Bovine 其他种属:Goat Human Monkey Mouse Pig Rat Sheep Chicken 待测物名称: Beta-defensin 1 缩写: DEFB1 检测范围: 3.12 ng/ml-200 ng/ml 灵敏度: 0.78 ng/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm

产品名称:人β-防御素1 ELISA Kit英文名称:Human β-defensins 1 ELISA Kit货号:CSB-E14186h 别名: BD1, DEFB-1, DEFB101, HBD1, MGC51822, beta-defensin-1 规格: 96T 产品类型:ELISA Kit中文价格: 3600  种属: Human 其他种属:Bovine Goat Monkey Mouse Pig Rat Sheep Chicken 待测物名称: defensin, beta 1 缩写: DEFB1 检测范围: 3.12 pg/ml-200 pg/ml 灵敏度: 0.78 pg/ml 反应时间: 1-5h

产品名称:人细胞色素C(Cyt-C)ELISA Kit 英文名称:Human Cytochrome-C,Cyt-C ELISA Kit 产品类型： ELISA Kit 货号： CSB-E08530h 价格： 3200 规格： 96T 种属： Human 样本类型： serum, plasma, cell lysates 检测范围： 7.8 ng/ml-500 ng/ml 灵敏度： 1.95 ng/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 用途： For research use only. Not for diagnostic use. 标准曲线： 产品名称                                        货号           规格 Mouse Cytochrome-C,Cyt-C ELISA Kit           CSB-E08532m       96T Rat Leukotriene C4,LT-C4 ELISA Kit           CSB-E08534r       96T Human Cytochrome-C,Cyt-C ELISA Kit           CSB-E08530h       96T Mouse Leukotriene C4,LT-C4 ELISA Kit         CSB-E08535m       96T Human Leukotriene C4,LT-C4 ELISA Kit         CSB-E08533h       96T Mouse Amylin ELISA Kit                       CSB-E08538m       96T Rat Amylin ELISA Kit                         CSB-E08537r       96T Human Amylin ELISA Kit                       CSB-E08536h       96T

产品名称:大鼠细胞色素C(Cyt-C)ELISA Kit 英文名称:Rat Cytochrome-C,Cyt-C ELISA Kit 产品类型： ELISA Kit 货号： CSB-E08531r 价格： 3900 规格： 96T 种属： Rat 样本类型： serum, plasma, cell culture supernates, tissue homogenates 检测范围： 31.2 pg/ml-2000 pg/ml 灵敏度： 7.8 pg/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 用途： For research use only. Not for diagnostic use. 产品名称                                        货号           规格 Mouse Cytochrome-C,Cyt-C ELISA Kit           CSB-E08532m       96T Rat Leukotriene C4,LT-C4 ELISA Kit           CSB-E08534r       96T Human Cytochrome-C,Cyt-C ELISA Kit           CSB-E08530h       96T Mouse Leukotriene C4,LT-C4 ELISA Kit         CSB-E08535m       96T Human Leukotriene C4,LT-C4 ELISA Kit         CSB-E08533h       96T Mouse Amylin ELISA Kit                       CSB-E08538m       96T Rat Amylin ELISA Kit                         CSB-E08537r       96T Human Amylin ELISA Kit                       CSB-E08536h       96T

产品名称:小鼠细胞色素C(Cyt-C)ELISA Kit 英文名称:Mouse Cytochrome-C,Cyt-C ELISA Kit 产品类型： ELISA Kit 货号： CSB-E08532m 价格： 3200 规格： 96T 种属： Mouse 样本类型： serum, plasma, tissue homogenates, cell lysates 检测范围： 34.3 pg/ml-2200 pg/ml 灵敏度： 8.5 pg/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 用途： For research use only. Not for diagnostic use. 原理： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYCS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C......＋This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYCS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYCS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYCS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYCS bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性： This assay has high sensitivity and excellent specificity for detection of Mouse CYCS. No significant cross-reactivity or interference between Mouse CYCS and analogues was observed. 精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......＋Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......＋Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......＋Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the CYCS concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 产品名称                                    货号                 规格 Pig Cytochrome c(CYCS) ELISA kit        CSB-EL006328PI           96T Rat Cytochrome c(CYCS) ELISA kit        CSB-EL006328RA           96T Human Cytochrome-C,Cyt-C ELISA Kit      CSB-E08530h              96T Sheep Cytochrome c(CYCS) ELISA kit      CSB-EL006328SH           96T Rabbit Cytochrome c(CYCS) ELISA kit     CSB-EL006328RB           96T Monkey Cytochrome c(CYCS) ELISA kit     CSB-EL006328RH           96T Horse Cytochrome c(CYCS) ELISA kit      CSB-EL006328HO           96T Donkey Cytochrome c(CYCS) ELISA kit     CSB-EL006328DK           96T Dog Cytochrome c(CYCS) ELISA kit        CSB-EL006328DO           96T Bovine Cytochrome c(CYCS) ELISA kit     CSB-EL006328BO           96T 蛋白 产品名称                                                          规格 Recombinant Xenopus tropicalis Cytochrome c, somatic(cycs)        1mg Recombinant Antirrhinum majus Transcription factor CYCLOIDEA(CYC) 1mg Recombinant Drosophila melanogaster Protein cycle(cyc)            1mg Recombinant Chlorobaculum parvum Photosynthetic reaction center cytochrome c-551(pscC) 1mg Recombinant Escherichia coli D-serine/D-alanine/glycine transporter(cycA) 1mg

CUSABIO 2013开学巨献   活动一   开学季，CUSABIO好礼送不停：凡订购任意2个CUSABIO试剂盒（96T），或购买指定类别任意产品满3500元即可获赠精美礼品一份 * 8G U盘 * 价值50元移动充值卡 * 价值50元DQ冰雪皇后冰淇淋缤纷卡 以上礼品，任选其一，多买多送！ 参与活动产品类别：   试剂盒    天然蛋白 多克隆抗体 单克隆抗体   RT-PCR引物对   活动二 开学季， CUSABIO好礼送不停之克隆，重组蛋白产品惊喜促销！ 活动期间，购买任意一款克隆，重组蛋白产品，即送30元移动充值卡一张，多买多送！ 参与活动产品类别：   克隆   重组蛋白       礼品多多，机会属于你！！   活动时间：2013年9月1日至2013年10月31日 本活动解释权---上海希美化学有限公司

产品名称:猪肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1) ELISA kit 英文名称:Pig hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit 产品类型： ELISA Kit 货号： CSB-EL010144PI 价格： 4200 规格： 96T 种属： Pig 样本类型： serum, plasma, tissue homogenates 检测范围： 0.18 ng/ml-12 ng/ml 灵敏度： 0.04 ng/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450nm 用途： For research use only. Not for diagnostic use. 原理： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any......＋This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAVCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAVCR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAVCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性： This assay has high sensitivity and excellent specificity for detection of Pig HAVCR1. No significant cross-reactivity or interference between Pig HAVCR1 and analogues was observed. 精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......＋Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......＋Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......＋Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HAVCR1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 产品名称  货号   规格 Monkey hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit  CSB-EL010144RH 96T Rat Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08808r 96T Human Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08807h 96T Mouse Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08809m 96T 蛋白 产品名称规格 Recombinant Human Hepatitis A virus cellular receptor 1(HAVCR1) 1mg Recombinant Chlorocebus aethiops Hepatitis A virus cellular receptor 1(HAVCR1) 1mg Recombinant Rat Hepatitis A virus cellular receptor 1 homolog(Havcr1) 1mg

产品名称:猴肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1) ELISA kit 英文名称:Monkey hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit 产品类型： ELISA Kit 货号： CSB-EL010144RH 价格： 4200 规格： 96T 种属： Monkey 样本类型： serum, urine 检测范围： 0.312 ng/ml-20 ng/ml 灵敏度： 0.078 ng/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450nm 用途： For research use only. Not for diagnostic use. 原理： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any......＋This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAVCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAVCR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAVCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性： This assay has high sensitivity and excellent specificity for detection of Monkey HAVCR1. No significant cross-reactivity or interference between Monkey HAVCR1 and analogues was observed. 精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......＋Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......＋Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......＋Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HAVCR1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 产品名称   货号    规格 Pig hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit  CSB-EL010144PI 96T Rat Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08808r 96T Human Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08807h 96T Mouse Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08809m 96T 蛋白 产品名称规格 Recombinant Human Hepatitis A virus cellular receptor 1(HAVCR1) 1mg Recombinant Chlorocebus aethiops Hepatitis A virus cellular receptor 1(HAVCR1) 1mg Recombinant Rat Hepatitis A virus cellular receptor 1 homolog(Havcr1) 1mg

产品名称:大鼠肾损伤分子1(Kim-1)ELISA Kit 英文名称:Rat Kidney injury molecule 1,Kim-1 ELISA Kit 产品类型： ELISA Kit 货号： CSB-E08808r 价格： 3900 规格： 96T 种属： Rat 样本类型： serum, plasma, urine 检测范围： 0.312 ng/ml-20 ng/ml 灵敏度： 0.078 ng/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450nm 用途： For research use only. Not for diagnostic use. 原理： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any......＋This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAVCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAVCR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAVCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性： This assay has high sensitivity and excellent specificity for detection of Rat HAVCR1. No significant cross-reactivity or interference between Rat HAVCR1 and analogues was observed. 精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......＋Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......＋Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......＋Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HAVCR1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   产品名称   货号    规格 Monkey hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit  CSB-EL010144RH 96T Pig hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit  CSB-EL010144PI 96T Human Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08807h 96T Mouse Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08809m 96T 蛋白 产品名称   规格 Recombinant Human Hepatitis A virus cellular receptor 1(HAVCR1) 1mg Recombinant Chlorocebus aethiops Hepatitis A virus cellular receptor 1(HAVCR1) 1mg Recombinant Rat Hepatitis A virus cellular receptor 1 homolog(Havcr1) 1mg

产品名称:人肾损伤分子1(Kim-1)ELISA Kit 英文名称:Human Kidney injury molecule 1,Kim-1 ELISA Kit 产品类型： ELISA Kit 货号： CSB-E08807h 价格： 3600 规格： 96T 种属： Human 样本类型： serum, urine, tissue homogenates 检测范围： 0.312 ng/ml-20 ng/ml 灵敏度： 0.078 ng/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450nm 用途： For research use only. Not for diagnostic use. 原理： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any......＋This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAVCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAVCR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAVCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性： This assay has high sensitivity and excellent specificity for detection of Human HAVCR1. No significant cross-reactivity or interference between Human HAVCR1 and analogues was observed. 精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......＋Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......＋Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......＋Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HAVCR1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   产品名称    货号    规格 Monkey hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit  CSB-EL010144RH 96T Pig hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit  CSB-EL010144PI 96T Rat Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08808r 96T Mouse Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08809m 96T 蛋白 产品名称    规格 Recombinant Human Hepatitis A virus cellular receptor 1(HAVCR1) 1mg Recombinant Chlorocebus aethiops Hepatitis A virus cellular receptor 1(HAVCR1) 1mg Recombinant Rat Hepatitis A virus cellular receptor 1 homolog(Havcr1) 1mg

产品名称:小鼠肾损伤分子1(Kim-1)ELISA Kit 英文名称:Mouse Kidney injury molecule 1,Kim-1 ELISA Kit 产品类型： ELISA Kit 货号： CSB-E08809m 价格： 3800 规格： 96T 种属： Mouse 检测范围： 0.94 ng/ml-60 ng/ml 灵敏度： 0.23 ng/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450nm 用途： For research use only. Not for diagnostic use. 原理： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any......＋This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAVCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAVCR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAVCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性： This assay has high sensitivity and excellent specificity for detection of Mouse HAVCR1. No significant cross-reactivity or interference between Mouse HAVCR1 and analogues was observed. 精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......＋Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......＋Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......＋Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HAVCR1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.    产品名称   英文名称     货号    规格 Monkey hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit    CSB-EL010144RH    96T Pig hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit       CSB-EL010144PI    96T Rat Kidney injury molecule 1,Kim-1 ELISA Kit                           CSB-E08808r       96T Human Kidney injury molecule 1,Kim-1 ELISA Kit                         CSB-E08807h       96T 蛋白 产品名称      规格 Recombinant Human Hepatitis A virus cellular receptor 1(HAVCR1)                  1mg Recombinant Chlorocebus aethiops Hepatitis A virus cellular receptor 1(HAVCR1)   1mg Recombinant Rat Hepatitis A virus cellular receptor 1 homolog(Havcr1)            1mg