重组人泛素(Recombinant Human Ubiquitin)

RPS27A Human
Ubiquitin Human Recombinant
Catalog No. PRO-314

Synonyms:
Ubiquitin, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A.

Introduction:
RPS27A is a small protein composed of 76 amino acids. RPS27A is found only in eukaryotic organisms among which shows strong sequence conservation. The RPS27A protein is present in all cell types, thus giving rise to its name.
RPS27A is found either in free form or conjugated to proteins through a covalent bond between the glycine at the C-terminal end and the side chains of lysine.
The connection of multiple copies of RPS27A targets the proteins for degradation by the 26S proteosome. RPS27A ligation is an ATP-dependent multi-step process. RPS27A is activated by the E1 enzyme. The attachment of RPS27A to the target protein is catalyzed by the E2 enzyme acting in concert with E3 which is involved in the recognition of the substrate protein.

Description:
Ubiquitin Human Recombinant expressed in Escherichia coli and purified by ion-exchange chromatography.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered solution.

Formulation:
1mg/ml containing 50mM HEPES (pH7.5) 150mM NaCl, 1mM DTT and 10% glycerol.

Purity:
Greater than 95% as estimated by analysis of Coomassie blue stained SDS-PAGE.

Stability:
Store at -20°C if intended use is longer than 1 week, store at 4°C for a period up to 1 week.
Please avoid freeze thaw cycles.

Sequence:
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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