人体钙激活酶-1(Human CAPN1)

CAPN1
Human Calpain-1 Catalytic Subunit
Catalog No. PRO-363

Synonyms:
Calpain-1 catalytic subunit, EC 3.4.22.52, Calpain-1 large subunit, Calcium-activated neutral proteinase 1, Calpain mu-type, muCANP, Micromolar-calpain, Cell proliferation-inducing gene 30 protein, CANP 1, CAPN1, CANPL1, PIG30, CANP, muCL, CANP1.

Introduction:
Calpain’s activity is attributed to two main isoforms: μ-calpain and m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (μ-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4.
CAPN1 is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of the substrates involved in cytoskeletal remodeling and signal transduction. CAPN1 is activated by micromolar concentrations of calcium and inhibited by calpastatin.

Description:
CAPN1 consists of an 80-kDa large subunit and a 30 kDa small subunit.
CAPN1 was purified by sequential chromatography through DEAE-Sepharose, A1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns.

Source:
Human Erythrocytes

Physical Appearance:
Sterile Filtered colorless solution.

Formulation:
50mM imidazole-HCl, 100mM NaCl, 5mM EGTA, 1mM DTT and 10% sucrose.

Stability:
CAPN1 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 90% as determined by SDS-PAGE.

Applications:
This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.
For adsorption we suggest the following procedure:

A- Dilute 1µl of the antiserum against µ-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1000).

B- Add 1µg of protein to 1ml of the diluted antibody solution and mix well.

C- Incubate for at least 6 hours in the cold.

D- Apply to tissue-sections and incubate for 3 days.

E - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).

As a result, the immunostaining should be strongly reduced or even completely prevented.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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