Recombinant Human Pancreas S Duodenum Homeobox-1(PDX-1)

PDX1 Human
Pancreatic Duodenal Homeobox-1 Human Recombinant
Catalog No. PRO-415

Synonyms:
Pancreas/duodenum homeobox protein 1, PDX-1, Insulin promoter factor 1, IPF-1, Islet/duodenum homeobox-1, IDX-1, Somatostatin-transactivating factor 1, STF-1, Insulin upstream factor 1, IUF-1, Glucose-sensitive factor, GSF, PDX1, IPF1, IUF1, MODY4.

Introduction:

PDX1 is a transcription factor which is expressed in beta and delta cells of the islets of Langerhans and in scattered endocrine cells of the duodenum. Pancreatic Duodenal Homeobox-1 activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. PDX1 is particularly involved in glucose-dependent regulation of insulin gene transcription. Furthermore, PDX1 plays a key part in the development of the pancreas and islet cell ontogeny. As a result homozygous interference with the gene in humans and mice results in pancreatic agenesis. The result of heterozygous mutations in PDX1 gene is impaired glucose tolerance and symptoms of diabetes as seen in MODY4 and late-onset Type II (non-insulin-dependent) diabetes mellitus. PDX1 binds preferentially the DNA motif 5'-[ct]taat[tg]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, IPF1 is required for maintaining the hormone-producing phenotype of the beta-cell. In adults PDX1 expression is increased in the pancreas duct cells that have been induced to proliferate and differentiate to form new islets. Deficiencies in pancreatic PDX1 could therefore contribute to Type II diabetes by affecting compensatory mechanisms that boost the rate of beta-cell neogenesis to meet the increased insulin secretory demand.

Description:
Pancreatic Duodenal Homeobox-1 Human Recombinant, produced in E. coli, is a 30.64kDa protein containing 283 amino acids.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
Lyophilized powder of liquid in PBS, pH 7.4.

Solubility:
It is recommended to reconstitute the lyophilized PDX1 in sterile PBS, 10% glycerol to prepare a stock of 100 μg/ml.

Stability:
Recombinant Human PDX1 although stable at 25°C 1 week, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.

Purity:
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity:
The activity of human PDX-1 is determined by the ability to stimulate NeuroD1 gene expression in WB cells and binding to insulin promoter be EMSA assay.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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