Bovine Neurofilament Medium Polypeptide(NEFM)

NEFM
Neurofilament Medium Polypeptide Bovine
Catalog No. PRO-523

Synonyms:
Neurofilament medium polypeptide, NF-M, Neurofilament triplet M protein, 160 kDa neurofilament protein, Neurofilament 3, NEFM, NEF3, NFM.

Introduction:
Neurofilaments are type IV intermediate filament heteropolymers that are composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber and may also have a role in intracellular transport to axons and dendrites.
NeuroFilament 160kDa is a medium neurofilament protein, which is commonly used as a biomarker of neuronal damage.

Description:
Ultra Pure NeuroFilament Protein having a Molecular mass of 160 kDa produced from Bovine Spinal Cord.

Source:
Bovine Spinal Cord

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein was lyophilized from a 1mg/ml solution containing 10mM sodium phosphate, pH-7.5, 2mM DTT, 6M urea and 1mM EDTA.

Solubility:
It is recommended to reconstitute the lyophilized NEFM in sterile 18MΩ-cm H2O.

Stability:
Lyophilized NEFM although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NEFM should be stored at 4°C between 2-7 days and for future use below
-18°C.
Please prevent freeze-thaw cycles.

Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Applications:
Protein standard in 1D and 2D SDS gelelectrophoresis
Immunoassays
Immunization

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.