重组人前列腺特异性基因1(Recombinant Human HPG1)

HPG-1 Human
Prostate Specific Gene-1 Human Recombinant
Catalog No. PRO-423

Synonyms:
Human Prostate-Specific Gene-1, HPG-1, HPG1.

Introduction:
Human Prostate-Specific Gene-1 (HPG-1) product is a recently discovered membrane-anchored protein of approximately 15 kD. It was detected exclusively in the prostate (19 different tissues were tested).
Antisense-oligonicleotide inhibition of HPG-1 expression inhibited the growth of one prostate cancer cell line (LNCaP) significantly (86%). HPG-1 might be a novel diagnostic and/or therapeutic target.

Description:
Recombinant HPG-1 is a 16 kDa recombinant fusion protein consisting of 127 amino acid residues of the human PG-1 and 9 additional amino acid residues of the His-Tag (underlined).
MKHHHHHHHM IKKNLKKLGI EETYLNIIKA IYDRPTASIQ KTENLSTKTG TSQEYHLSPL WFYIVLEILA STIRQEKNLK DIHTEKEEVK LSLFADAMIL YLKKPNDSTR KLLELIKKIH SSCRIKINIH FAFCSQ

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M MES buffer pH 6.5.

Solubility:
Add 0.2 ml of dH2O and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited.

Stability:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
The lyophilized protein remains stable until the expiry date when stored at -20°C.

Purity:
Greater than 95% as determined by SDS-PAGE.

Specificity:
The amino acid sequence of the recombinant human PG-1 is 100% homologous to the amino acid sequence of the HPG-1.

Purification Method:
Ni-NTA affinity chromatography.

Applications:
Western blotting, ELISA

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.